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Article: Absence of CFTR is associated with pleiotropic effects on mucins in mouse gallbladder epithelial cells

TitleAbsence of CFTR is associated with pleiotropic effects on mucins in mouse gallbladder epithelial cells
Authors
KeywordsBiliary tract
Cystic fibrosis transmembrane conductance regulator
Exocytosis
Issue Date2006
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpgi.physiology.org/
Citation
American Journal Of Physiology - Gastrointestinal And Liver Physiology, 2006, v. 291 n. 6, p. G1148-G1154 How to Cite?
AbstractMucus of cystic fibrosis patients exhibits altered biochemical composition and biophysical behavior, but the causal relationships between altered cystic fibrosis transmembrane conductance regulator (CFTR) function and the abnormal mucus seen in various organ systems remain unclear. We used cultured gallbladder epithelial cells (GBEC) from wild-type and Cftr(-/-) mice to investigate mucin gene and protein expression, kinetics of postexocytotic mucous granule content expansion, and biochemical and ionic compositions of secreted mucins. Muc1, Muc3, Muc4, Muc5ac, and Muc5b mRNA levels were significantly lower in Cftr(-/-) GBEC compared with wild-type cells, whereas Muc2 mRNA levels were higher in Cftr(-/-) cells. Quantitative immunoblotting demonstrated a trend toward lower MUC1, MUC2, MUC3, MUC5AC, and MUC5B mucin levels in Cftr(-/-) cells compared with cells from wild-type mice. In contrast, the levels of secreted MUC1, MUC3, MUC5B, and MUC6 mucins were significantly higher from Cftr(-/-) cells; a trend toward higher levels of secreted MUC2 and MUC5AC was also noted from Cftr(-/-) cells. Cftr(-/-) cells demonstrated slower postexocytotic mucous granule content expansion. Calcium concentration was significantly elevated in the mucous gel secreted by Cftr(-/-) cells compared with wild-type cells. Secreted mucins from Cftr(-/-) cells contained higher sulfate concentrations. Thus absence of CFTR is associated with pleiotropic effects on mucins in murine GBEC. Copyright © 2005 by the American Physiological Society.
Persistent Identifierhttp://hdl.handle.net/10722/92462
ISSN
2015 Impact Factor: 3.297
2015 SCImago Journal Rankings: 1.936
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKuver, Ren_HK
dc.contributor.authorWong, Ten_HK
dc.contributor.authorKlinkspoor, JHen_HK
dc.contributor.authorLee, SPen_HK
dc.date.accessioned2010-09-17T10:46:59Z-
dc.date.available2010-09-17T10:46:59Z-
dc.date.issued2006en_HK
dc.identifier.citationAmerican Journal Of Physiology - Gastrointestinal And Liver Physiology, 2006, v. 291 n. 6, p. G1148-G1154en_HK
dc.identifier.issn0193-1857en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92462-
dc.description.abstractMucus of cystic fibrosis patients exhibits altered biochemical composition and biophysical behavior, but the causal relationships between altered cystic fibrosis transmembrane conductance regulator (CFTR) function and the abnormal mucus seen in various organ systems remain unclear. We used cultured gallbladder epithelial cells (GBEC) from wild-type and Cftr(-/-) mice to investigate mucin gene and protein expression, kinetics of postexocytotic mucous granule content expansion, and biochemical and ionic compositions of secreted mucins. Muc1, Muc3, Muc4, Muc5ac, and Muc5b mRNA levels were significantly lower in Cftr(-/-) GBEC compared with wild-type cells, whereas Muc2 mRNA levels were higher in Cftr(-/-) cells. Quantitative immunoblotting demonstrated a trend toward lower MUC1, MUC2, MUC3, MUC5AC, and MUC5B mucin levels in Cftr(-/-) cells compared with cells from wild-type mice. In contrast, the levels of secreted MUC1, MUC3, MUC5B, and MUC6 mucins were significantly higher from Cftr(-/-) cells; a trend toward higher levels of secreted MUC2 and MUC5AC was also noted from Cftr(-/-) cells. Cftr(-/-) cells demonstrated slower postexocytotic mucous granule content expansion. Calcium concentration was significantly elevated in the mucous gel secreted by Cftr(-/-) cells compared with wild-type cells. Secreted mucins from Cftr(-/-) cells contained higher sulfate concentrations. Thus absence of CFTR is associated with pleiotropic effects on mucins in murine GBEC. Copyright © 2005 by the American Physiological Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpgi.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Gastrointestinal and Liver Physiologyen_HK
dc.subjectBiliary tracten_HK
dc.subjectCystic fibrosis transmembrane conductance regulatoren_HK
dc.subjectExocytosisen_HK
dc.titleAbsence of CFTR is associated with pleiotropic effects on mucins in mouse gallbladder epithelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, SP: sumlee@hku.hken_HK
dc.identifier.authorityLee, SP=rp01351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1152/ajpgi.00547.2005en_HK
dc.identifier.pmid16825704-
dc.identifier.scopuseid_2-s2.0-33845420710en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33845420710&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume291en_HK
dc.identifier.issue6en_HK
dc.identifier.spageG1148en_HK
dc.identifier.epageG1154en_HK
dc.identifier.eissn1522-1547-
dc.identifier.isiWOS:000241855700017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKuver, R=6701723533en_HK
dc.identifier.scopusauthoridWong, T=15137295300en_HK
dc.identifier.scopusauthoridKlinkspoor, JH=6602590656en_HK
dc.identifier.scopusauthoridLee, SP=7601417497en_HK

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