Article: Identification of retinoic acid-regulated nuclear matrix-associated protein as a novel regulator of gastric cancer

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Title	Identification of retinoic acid-regulated nuclear matrix-associated protein as a novel regulator of gastric cancer
Authors	Li, J3 Ng, EKO3 Ng, YP2 Wong, CYP3 Yu, J3 Jin, H3 Cheng, VYY4 Go, MYY3 Cheung, PKF3 Ebert, MPA2 Tong, J3 To, KF3 Chan, FKL3 Sung, JJY3 Ip, NY2 Leung, WK1 3
Keywords	DTL/RAMP L2DTL Oncogene P21 P53 Tumourigenesis
Issue Date	2009
Publisher	Nature Publishing Group. The Journal's web site is located at http://www.nature.com/bjc
Citation	British Journal Of Cancer, 2009, v. 101 n. 4, p. 691-698 [How to Cite?] DOI: http://dx.doi.org/10.1038/sj.bjc.6605202
Abstract	Background: Retinoic acid-regulated nuclear matrix-associated protein (RAMP) is a WD40 repeat-containing protein that is involved in various biological functions, but little is known about its role in human cancer. This study aims to delineate the oncogenic role of RAMP in gastric carcinogenesis.Methods: RAMP expression was examined by real-time quantitative RT-PCR, immunohistochemistry and western blotting. Inhibition of RAMP expression was performed by siRNA-mediated knockdown. The functional effects of RAMP on cell kinetics were measured by cell viability assay, colony formation assay and flow cytometry. Cell lines stably expressing RAMP were established to investigate the oncogenic effects of RAMP in vitro.Results: Ramp was readily expressed in all seven gastric cancer cell lines and was significantly increased in human gastric cancer tissues when compared with their adjacent non-cancerous tissues (P0.001). In keeping with this, expression of RAMP protein was higher in gastric cancer tissues compared with their adjacent non-cancerous tissues, whereas moderate protein expression were noted in intestinal metaplasia. Knockdown of RAMP in gastric cancer cells significantly reduced cell proliferation (P0.01) and soft agar colony formation (P0.001), but induced apoptosis and G 2 /M arrest. In additional, knockdown RAMP induced cell apoptosis is dependent on functional accumulation of p53 and p21 and induction of cleaved caspases-9, caspases-3 and PARP. Strikingly, overexpression of RAMP promoted anchorage-independent cell growth in soft agar.Conclusion: Our findings demonstrate that RAMP plays an oncogenic role in gastric carcinogenesis. Inhibition of RAMP may be a promising approach for gastric cancer therapy. © 2009 Cancer Research UK.
ISSN	0007-0920 2011 Impact Factor: 5.042 2011 SCImago Journal Rankings: 0.561
DOI	http://dx.doi.org/10.1038/sj.bjc.6605202
ISI Accession Number ID	WOS:000268861000019
PubMed Central ID	PMC2736823
References	References in Scopus

DC Field	Value
dc.contributor.author	Li, J
dc.contributor.author	Ng, EKO
dc.contributor.author	Ng, YP
dc.contributor.author	Wong, CYP
dc.contributor.author	Yu, J
dc.contributor.author	Jin, H
dc.contributor.author	Cheng, VYY
dc.contributor.author	Go, MYY
dc.contributor.author	Cheung, PKF
dc.contributor.author	Ebert, MPA
dc.contributor.author	Tong, J
dc.contributor.author	To, KF
dc.contributor.author	Chan, FKL
dc.contributor.author	Sung, JJY
dc.contributor.author	Ip, NY
dc.contributor.author	Leung, WK
dc.date.accessioned	2010-09-17T10:43:17Z
dc.date.available	2010-09-17T10:43:17Z
dc.date.issued	2009
dc.description.abstract	Background: Retinoic acid-regulated nuclear matrix-associated protein (RAMP) is a WD40 repeat-containing protein that is involved in various biological functions, but little is known about its role in human cancer. This study aims to delineate the oncogenic role of RAMP in gastric carcinogenesis.Methods: RAMP expression was examined by real-time quantitative RT-PCR, immunohistochemistry and western blotting. Inhibition of RAMP expression was performed by siRNA-mediated knockdown. The functional effects of RAMP on cell kinetics were measured by cell viability assay, colony formation assay and flow cytometry. Cell lines stably expressing RAMP were established to investigate the oncogenic effects of RAMP in vitro.Results: Ramp was readily expressed in all seven gastric cancer cell lines and was significantly increased in human gastric cancer tissues when compared with their adjacent non-cancerous tissues (P0.001). In keeping with this, expression of RAMP protein was higher in gastric cancer tissues compared with their adjacent non-cancerous tissues, whereas moderate protein expression were noted in intestinal metaplasia. Knockdown of RAMP in gastric cancer cells significantly reduced cell proliferation (P0.01) and soft agar colony formation (P0.001), but induced apoptosis and G 2 /M arrest. In additional, knockdown RAMP induced cell apoptosis is dependent on functional accumulation of p53 and p21 and induction of cleaved caspases-9, caspases-3 and PARP. Strikingly, overexpression of RAMP promoted anchorage-independent cell growth in soft agar.Conclusion: Our findings demonstrate that RAMP plays an oncogenic role in gastric carcinogenesis. Inhibition of RAMP may be a promising approach for gastric cancer therapy. © 2009 Cancer Research UK.
dc.description.nature	published_or_final_version
dc.identifier.citation	British Journal Of Cancer, 2009, v. 101 n. 4, p. 691-698 [How to Cite?] DOI: http://dx.doi.org/10.1038/sj.bjc.6605202
dc.identifier.doi	http://dx.doi.org/10.1038/sj.bjc.6605202
dc.identifier.epage	698
dc.identifier.hkuros	168713
dc.identifier.isi	WOS:000268861000019
dc.identifier.issn	0007-0920 2011 Impact Factor: 5.042 2011 SCImago Journal Rankings: 0.561
dc.identifier.issue	4
dc.identifier.openurl
dc.identifier.pmcid	PMC2736823
dc.identifier.pmid	19672268
dc.identifier.scopus	eid_2-s2.0-68749109533
dc.identifier.spage	691
dc.identifier.uri	http://hdl.handle.net/10722/92344
dc.identifier.volume	101
dc.language	eng
dc.publisher	Nature Publishing Group. The Journal's web site is located at http://www.nature.com/bjc
dc.publisher.place	United Kingdom
dc.relation.ispartof	British Journal of Cancer
dc.relation.references	References in Scopus
dc.subject.mesh	Apoptosis - physiology
dc.subject.mesh	Immunohistochemistry
dc.subject.mesh	Nuclear Proteins - genetics - metabolism
dc.subject.mesh	Stomach Neoplasms - genetics - metabolism
dc.subject.mesh	Tumor Markers, Biological - analysis
dc.subject	DTL/RAMP
dc.subject	L2DTL
dc.subject	Oncogene
dc.subject	P21
dc.subject	P53
dc.subject	Tumourigenesis
dc.title	Identification of retinoic acid-regulated nuclear matrix-associated protein as a novel regulator of gastric cancer
dc.type	Article

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Author Affiliations

Prince of Wales Hospital Hong Kong
Hong Kong University of Science and Technology
Chinese University of Hong Kong
Technische Universität München