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Article: The expression of hGCN5 in burkitt lymphma Daudi cells and effets of TSA rgulating cell antiproliferation and apoptosis

TitleThe expression of hGCN5 in burkitt lymphma Daudi cells and effets of TSA rgulating cell antiproliferation and apoptosis
Authors
KeywordsDaudi
Hgcn5
Lymphoma
Tsa
Issue Date2005
PublisherTianjin Yike Daxue Fushu Zhongliu Yiyuan. The Journal's web site is located at http://zgzllc-e.periodicals.net.cn/
Citation
Chinese Journal of Clinical Oncology, 2005, v. 32 n. 16, p. 901-905 How to Cite?
AbstractObjective: To investigate the effect of TSA (Trichostatin A) regulating hGCN5 (human general control of amino acid synthesis protein 5) in B-NHL cell line Daudi cells, and to study anti-proliferation molecular machenism of TSA on B-NHL. Methods: The cell proliferation was measured by MTT assay. The apoptosis rates and cell cycle were determined with flow cytometry. The expression of hGCN5 in treated Daudi cells was determined by immunocytochemistry and Western blot. Results: The proliferation of Daudi cells was decreased in TSA-treated group. Treatment with TSA(200μg/L) and TSA (400μg/L) for 24h, the apoptosis rates of Daudi cells were (14.74±2.04)% and(17.63±1.25)% respectively. The cell cycle was arrested in G0/G1 phase (50, 100μg/L) and in G2/M phase (200μg/L) for 24h. The expression of hGCN5 in Daudi cells was increased in TSA group (400μg/L) for 24h compared with control group by immunocytochemistry and Western blot (P<0.05). Conclusion: TSA can increase the expression of hGCN5 in Daudi cells and it plays an important role in regulating B-NHL cell line Daudi cells proliferation effects.
Persistent Identifierhttp://hdl.handle.net/10722/92319
ISSN
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Hen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorLi, Xen_HK
dc.date.accessioned2010-09-17T10:42:32Z-
dc.date.available2010-09-17T10:42:32Z-
dc.date.issued2005en_HK
dc.identifier.citationChinese Journal of Clinical Oncology, 2005, v. 32 n. 16, p. 901-905en_HK
dc.identifier.issn1672-7118en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92319-
dc.description.abstractObjective: To investigate the effect of TSA (Trichostatin A) regulating hGCN5 (human general control of amino acid synthesis protein 5) in B-NHL cell line Daudi cells, and to study anti-proliferation molecular machenism of TSA on B-NHL. Methods: The cell proliferation was measured by MTT assay. The apoptosis rates and cell cycle were determined with flow cytometry. The expression of hGCN5 in treated Daudi cells was determined by immunocytochemistry and Western blot. Results: The proliferation of Daudi cells was decreased in TSA-treated group. Treatment with TSA(200μg/L) and TSA (400μg/L) for 24h, the apoptosis rates of Daudi cells were (14.74±2.04)% and(17.63±1.25)% respectively. The cell cycle was arrested in G0/G1 phase (50, 100μg/L) and in G2/M phase (200μg/L) for 24h. The expression of hGCN5 in Daudi cells was increased in TSA group (400μg/L) for 24h compared with control group by immunocytochemistry and Western blot (P<0.05). Conclusion: TSA can increase the expression of hGCN5 in Daudi cells and it plays an important role in regulating B-NHL cell line Daudi cells proliferation effects.en_HK
dc.languageengen_HK
dc.publisherTianjin Yike Daxue Fushu Zhongliu Yiyuan. The Journal's web site is located at http://zgzllc-e.periodicals.net.cn/en_HK
dc.relation.ispartofChinese Journal of Clinical Oncologyen_HK
dc.subjectDaudien_HK
dc.subjectHgcn5en_HK
dc.subjectLymphomaen_HK
dc.subjectTsaen_HK
dc.titleThe expression of hGCN5 in burkitt lymphma Daudi cells and effets of TSA rgulating cell antiproliferation and apoptosisen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, Y:ychenc@hkucc.hku.hken_HK
dc.identifier.authorityChen, Y=rp1318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-35948959046en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-35948959046&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume32en_HK
dc.identifier.issue16en_HK
dc.identifier.spage901en_HK
dc.identifier.epage905en_HK

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