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Article: Characterization of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) in Shanghai, China: Molecular and cytogenetic characteristics, IgV gene restriction and hypermutation patterns

TitleCharacterization of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) in Shanghai, China: Molecular and cytogenetic characteristics, IgV gene restriction and hypermutation patterns
Authors
Keywords14Q32/Igh
Chronic Lymphocytic Leukemia
Cytogenetics
Fluorescence In Situ Hybridization
Igv Genes
Small Lymphocytic Lymphoma
Somatic Hypermutation
Issue Date2009
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/leukres
Citation
Leukemia Research, 2009, v. 33 n. 12, p. 1599-1603 How to Cite?
AbstractThe clinical, cytogenetic and molecular features of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), a disease previously considered to be rare in Asia, were examined in consecutive series of 70 cases diagnosed by our laboratory over a 30-month period. Clonal abnormalities were observed in 80% of CLL/SLL cases using a combination of conventional cytogenetic and fluorescence in situ hybridization (FISH) analysis. Those involving 14q32/IGH were the most frequent (24 cases), followed by trisomy 12 and 11q abnormalities. IgVH gene usage was non-random with over-representation of VH4-34, VH3-23 and a previously unreported increase in VH3-48 gene use. Somatic hypermutation (SHM) of IgVH germline sequences was observed in 56.5% of cases with stereotyped patterns of SHM observed in VH4-34 heavy chain complimentary-determining (HCDR1) and framework region CFR2 sequences. These findings in a Chinese population suggest subtle geographical differences in IgVH gene usage while the remarkably specific pattern of SHM suggest that a relatively limited set of antigens may be involved in the development of this disease worldwide. IgVH gene mutation status was a significant predictor of initial survival in CLL/SLL. However, an influence of karyotype on prognosis was not observed. © 2009 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92310
ISSN
2015 Impact Factor: 2.606
2015 SCImago Journal Rankings: 1.043
ISI Accession Number ID
Funding AgencyGrant Number
Benzene Health Consortium
Hua Shan HospitalHuang Pu District Hospital
Long March Hospital
Ren Ji Hospital
Rui Jin Hospital
Shanghai No. I People's Hospital
Shanghai Tumor Hospital
Shu Guang Hospital,
Zhong Shan Hospital
Funding Information:

We are grateful to the staff of the Fudan-Cinpathogen Clinical and Molecular Research Laboratory (CMRL), especially Henjuan Sun, Lihong Yao, Saijuan Zhu, Xiaohong Guo, and Chunhua Lin for skillful technical assistance and Junfang Xie and Ann Louden for their clerical assistance. Funding for the operation of CMRL was provided by the Benzene Health Consortium. Participating hospitals include: Hua Shan Hospital, Huang Pu District Hospital, Long March Hospital, Ren Ji Hospital, Rui Jin Hospital, Shanghai No. I People's Hospital, Shanghai Tumor Hospital, Shu Guang Hospital, and Zhong Shan Hospital.

References

 

DC FieldValueLanguage
dc.contributor.authorIrons, RDen_HK
dc.contributor.authorLe, Aen_HK
dc.contributor.authorBao, Len_HK
dc.contributor.authorZhu, Xen_HK
dc.contributor.authorRyder, Jen_HK
dc.contributor.authorWang, XQen_HK
dc.contributor.authorJi, Men_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorWu, Xen_HK
dc.contributor.authorLin, Gen_HK
dc.date.accessioned2010-09-17T10:42:16Z-
dc.date.available2010-09-17T10:42:16Z-
dc.date.issued2009en_HK
dc.identifier.citationLeukemia Research, 2009, v. 33 n. 12, p. 1599-1603en_HK
dc.identifier.issn0145-2126en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92310-
dc.description.abstractThe clinical, cytogenetic and molecular features of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), a disease previously considered to be rare in Asia, were examined in consecutive series of 70 cases diagnosed by our laboratory over a 30-month period. Clonal abnormalities were observed in 80% of CLL/SLL cases using a combination of conventional cytogenetic and fluorescence in situ hybridization (FISH) analysis. Those involving 14q32/IGH were the most frequent (24 cases), followed by trisomy 12 and 11q abnormalities. IgVH gene usage was non-random with over-representation of VH4-34, VH3-23 and a previously unreported increase in VH3-48 gene use. Somatic hypermutation (SHM) of IgVH germline sequences was observed in 56.5% of cases with stereotyped patterns of SHM observed in VH4-34 heavy chain complimentary-determining (HCDR1) and framework region CFR2 sequences. These findings in a Chinese population suggest subtle geographical differences in IgVH gene usage while the remarkably specific pattern of SHM suggest that a relatively limited set of antigens may be involved in the development of this disease worldwide. IgVH gene mutation status was a significant predictor of initial survival in CLL/SLL. However, an influence of karyotype on prognosis was not observed. © 2009 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/leukresen_HK
dc.relation.ispartofLeukemia Researchen_HK
dc.subject14Q32/Ighen_HK
dc.subjectChronic Lymphocytic Leukemiaen_HK
dc.subjectCytogeneticsen_HK
dc.subjectFluorescence In Situ Hybridizationen_HK
dc.subjectIgv Genesen_HK
dc.subjectSmall Lymphocytic Lymphomaen_HK
dc.subjectSomatic Hypermutationen_HK
dc.titleCharacterization of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) in Shanghai, China: Molecular and cytogenetic characteristics, IgV gene restriction and hypermutation patternsen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, Y:ychenc@hkucc.hku.hken_HK
dc.identifier.authorityChen, Y=rp1318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.leukres.2008.12.021en_HK
dc.identifier.pmid19428103-
dc.identifier.scopuseid_2-s2.0-70249148920en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70249148920&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume33en_HK
dc.identifier.issue12en_HK
dc.identifier.spage1599en_HK
dc.identifier.epage1603en_HK
dc.identifier.isiWOS:000270776500004-
dc.identifier.citeulike4823361-

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