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Article: Expression of hnRNP K in lung adenocarcinoma cells

TitleExpression of hnRNP K in lung adenocarcinoma cells
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2008
Citation
Journal of Sichuan University (Medical Science Edition), 2008, v. 39 n. 5, p. 823-826 How to Cite?
AbstractObjective To examine the effects of hnRNP K on the growth and apoptosis of lung adencarcinoma cells in vitro. Methods The expressions of hnRNP K were detected by the SP method of immunohistochemistry in 70 lung adencarcinoma tissue samples. The hnRNP K siRNA was transfected into human lung adencarcinoma cell line A549 with Lipofectamine 2000. The cells transfected with siRNAn and untreated served as controls. The inhibitory effect of siRNA on the expression of hnRNP K mRNA and protein was detected by RT-PCR and Western blot. The change in cell cycling and cell apoptosis of siRNA-treated cells was detected by flow cytometry. Results Positive expressions of hnRNP K were found in 38. 5%, 95. 2% and 91. 7% of lung adencarcinoma tissues in tumors with a diameter of ≤3 cm, 3-5 cm and ≥5 cm respectively (P< 0.01). The expressions of hnRNP K mRNA were significantly inhibited in siRNA- transfected cells compared with those in controls (P <0. 05). hnRNP K protein decreased in hnRNP K siRNA-transfected cells. siRNA targeting human hnRNP K inhibited the growth of lung adencarcinoma cell line A549 and the distribution of the cell cycle, with an apoptosis rate of 4. 79%. Twenty four hours after transfection, the number of cells in G 0/G1 phase increased from 37. 21% to 85. 60%, while the number of cells in S and G2/M phases decreased from 47. 71% and 13. 00% to 13. 50% and 0. 32%, respectively. Conclusion The expression of hnRNP K is closely correlated with the size of lung adencarcinoma. hnRNP K siRNA inhibits the growth and increases the apoptosis of lung adencarcinoma cells.
Persistent Identifierhttp://hdl.handle.net/10722/92309
ISSN
2015 SCImago Journal Rankings: 0.140
References

 

DC FieldValueLanguage
dc.contributor.authorTang, F-Men_HK
dc.contributor.authorLi, W-Men_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorWang, D-Men_HK
dc.contributor.authorHan, Jen_HK
dc.date.accessioned2010-09-17T10:42:14Z-
dc.date.available2010-09-17T10:42:14Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal of Sichuan University (Medical Science Edition), 2008, v. 39 n. 5, p. 823-826en_HK
dc.identifier.issn1672-173Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92309-
dc.description.abstractObjective To examine the effects of hnRNP K on the growth and apoptosis of lung adencarcinoma cells in vitro. Methods The expressions of hnRNP K were detected by the SP method of immunohistochemistry in 70 lung adencarcinoma tissue samples. The hnRNP K siRNA was transfected into human lung adencarcinoma cell line A549 with Lipofectamine 2000. The cells transfected with siRNAn and untreated served as controls. The inhibitory effect of siRNA on the expression of hnRNP K mRNA and protein was detected by RT-PCR and Western blot. The change in cell cycling and cell apoptosis of siRNA-treated cells was detected by flow cytometry. Results Positive expressions of hnRNP K were found in 38. 5%, 95. 2% and 91. 7% of lung adencarcinoma tissues in tumors with a diameter of ≤3 cm, 3-5 cm and ≥5 cm respectively (P< 0.01). The expressions of hnRNP K mRNA were significantly inhibited in siRNA- transfected cells compared with those in controls (P <0. 05). hnRNP K protein decreased in hnRNP K siRNA-transfected cells. siRNA targeting human hnRNP K inhibited the growth of lung adencarcinoma cell line A549 and the distribution of the cell cycle, with an apoptosis rate of 4. 79%. Twenty four hours after transfection, the number of cells in G 0/G1 phase increased from 37. 21% to 85. 60%, while the number of cells in S and G2/M phases decreased from 47. 71% and 13. 00% to 13. 50% and 0. 32%, respectively. Conclusion The expression of hnRNP K is closely correlated with the size of lung adencarcinoma. hnRNP K siRNA inhibits the growth and increases the apoptosis of lung adencarcinoma cells.en_HK
dc.languageengen_HK
dc.relation.ispartofJournal of Sichuan University (Medical Science Edition)en_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.titleExpression of hnRNP K in lung adenocarcinoma cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, Y:ychenc@hkucc.hku.hken_HK
dc.identifier.authorityChen, Y=rp1318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid19024324-
dc.identifier.scopuseid_2-s2.0-53349165262en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-53349165262&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue5en_HK
dc.identifier.spage823en_HK
dc.identifier.epage826en_HK

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