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Article: Characterization of a brain-specific nuclear LIM domain protein (FHL1B) which is an alternatively spliced variant of FHL1

TitleCharacterization of a brain-specific nuclear LIM domain protein (FHL1B) which is an alternatively spliced variant of FHL1
Authors
KeywordsBrain
Brain cDNA
FHL1B
RBP-J
SLIM1
Xq27
Zinc finger protein
Issue Date1999
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gene
Citation
Gene, 1999, v. 237 n. 1, p. 253-263 How to Cite?
AbstractWe have amplified and sequenced a novel, alternatively spliced variant of a human gene coding for the four-and-a-half LIM domain protein 1 ( FHL1). This gene is located at chromosome Xq27 and the spliced variant is named FHL1B. The ORF of FHL1B cDNA codes for a LIM-only protein that possesses a zinc finger and three tandem repeats of LIM domains at the N-terminus with an active bipartite nuclear localization signal (NLS) motif and a possible RBP-J binding region at the C-terminus. FHL1B and FHL1 have the same N-terminal three-and-a-half LIM domains but different C-terminal protein sequences, due to the presence of an additional alternative exon 4b in FHL1B causing a frame-shift in the 3'coding region. RT-PCR results revealed that the expression of FHL1 is not restricted in skeletal muscle and heart, but is widely distributed in other tissues, including brain, placenta, lung, liver, kidney and pancreas, albeit as a low abundance transcript. In contrast, FHL1B is specifically expressed in brain. The C-terminal alternative region in FHL1B is sufficient to localize FHL1B in the nucleus of mammalian cell. FHL1B is probably related to neural differentiation and certain fragile X syndrome. 1999 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/92295
ISSN
2015 Impact Factor: 2.319
2015 SCImago Journal Rankings: 1.043
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, SMYen_HK
dc.contributor.authorLi, HYen_HK
dc.contributor.authorNg, EKOen_HK
dc.contributor.authorOr, SMWen_HK
dc.contributor.authorChan, KKen_HK
dc.contributor.authorKotaka, Men_HK
dc.contributor.authorChim, SSCen_HK
dc.contributor.authorTsui, SKWen_HK
dc.contributor.authorWaye, MMYen_HK
dc.contributor.authorFung, KPen_HK
dc.contributor.authorLee, CYen_HK
dc.date.accessioned2010-09-17T10:41:49Z-
dc.date.available2010-09-17T10:41:49Z-
dc.date.issued1999en_HK
dc.identifier.citationGene, 1999, v. 237 n. 1, p. 253-263en_HK
dc.identifier.issn0378-1119en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92295-
dc.description.abstractWe have amplified and sequenced a novel, alternatively spliced variant of a human gene coding for the four-and-a-half LIM domain protein 1 ( FHL1). This gene is located at chromosome Xq27 and the spliced variant is named FHL1B. The ORF of FHL1B cDNA codes for a LIM-only protein that possesses a zinc finger and three tandem repeats of LIM domains at the N-terminus with an active bipartite nuclear localization signal (NLS) motif and a possible RBP-J binding region at the C-terminus. FHL1B and FHL1 have the same N-terminal three-and-a-half LIM domains but different C-terminal protein sequences, due to the presence of an additional alternative exon 4b in FHL1B causing a frame-shift in the 3'coding region. RT-PCR results revealed that the expression of FHL1 is not restricted in skeletal muscle and heart, but is widely distributed in other tissues, including brain, placenta, lung, liver, kidney and pancreas, albeit as a low abundance transcript. In contrast, FHL1B is specifically expressed in brain. The C-terminal alternative region in FHL1B is sufficient to localize FHL1B in the nucleus of mammalian cell. FHL1B is probably related to neural differentiation and certain fragile X syndrome. 1999 Elsevier Science B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/geneen_HK
dc.relation.ispartofGeneen_HK
dc.subjectBrainen_HK
dc.subjectBrain cDNAen_HK
dc.subjectFHL1Ben_HK
dc.subjectRBP-Jen_HK
dc.subjectSLIM1en_HK
dc.subjectXq27en_HK
dc.subjectZinc finger proteinen_HK
dc.titleCharacterization of a brain-specific nuclear LIM domain protein (FHL1B) which is an alternatively spliced variant of FHL1en_HK
dc.typeArticleen_HK
dc.identifier.emailNg, EKO: ngko@hku.hken_HK
dc.identifier.emailKotaka, M: masayo@hku.hken_HK
dc.identifier.authorityNg, EKO=rp01364en_HK
dc.identifier.authorityKotaka, M=rp00293en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0378-1119(99)00251-6en_HK
dc.identifier.pmid10524257-
dc.identifier.scopuseid_2-s2.0-0032865487en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032865487&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume237en_HK
dc.identifier.issue1en_HK
dc.identifier.spage253en_HK
dc.identifier.epage263en_HK
dc.identifier.isiWOS:000082500700028-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLee, SMY=35233892600en_HK
dc.identifier.scopusauthoridLi, HY=12762326800en_HK
dc.identifier.scopusauthoridNg, EKO=21135553700en_HK
dc.identifier.scopusauthoridOr, SMW=7006594257en_HK
dc.identifier.scopusauthoridChan, KK=7406034649en_HK
dc.identifier.scopusauthoridKotaka, M=6604073578en_HK
dc.identifier.scopusauthoridChim, SSC=6701728226en_HK
dc.identifier.scopusauthoridTsui, SKW=7004961364en_HK
dc.identifier.scopusauthoridWaye, MMY=7006687733en_HK
dc.identifier.scopusauthoridFung, KP=7202934739en_HK
dc.identifier.scopusauthoridLee, CY=7410142857en_HK

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