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Article: Quantitative Epstein-Barr virus DNA analysis and detection of gene promoter hypermethylation in nasopharyngeal (NP) brushing samples from patients with NP carcinoma

TitleQuantitative Epstein-Barr virus DNA analysis and detection of gene promoter hypermethylation in nasopharyngeal (NP) brushing samples from patients with NP carcinoma
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2002
PublisherAmerican Association for Cancer Research
Citation
Clinical Cancer Research, 2002, v. 8 n. 8, p. 2612-2619 How to Cite?
AbstractPurpose: Nasopharyngeal carcinoma (NPC) is highly prevalent in southern China and characterized by a strong association with EBV. We aimed to detect EBV DNA and cancer-related gene promoter hypermethylation in nasopharyngeal (NP) brushing samples and provide a novel noninvasive approach for NPC detection. Experimental Design: Twenty-eight NPC cases and 26 noncancerous subjects were prospectively recruited. NP brushing samples were subjected to quantitative real-time PCR analysis of EBV DNA and methylation-specific PCR analysis of the DAP-kinase, RASSF1A, and p16 genes. Results: EBV DNA quantity in NP brushing samples from NPC patients (median, 8.94 copies/actin) was significantly higher than that of controls (median, 0 copies/actin; P < 0.0001). Twenty-seven of 28 NPC patients had detectable EBV DNA in NP brushes, whereas 25 of 26 controls had undetectable or very low levels of EBV DNA. Elevated EBV DNA level in brushing samples as a tumor marker had a sensitivity of 96.4% and a specificity of 96.2% for NPC detection. Moreover, T 1 disease had a significantly lower EBV DNA level as compared with locally more advanced disease (P = 0.037). In brushing samples of NPC patients, the frequencies of DAP-kinase, RASSF1A, and p16 promoter hypermethylation were 50.0%, 39.3%, and 46.4%, respectively. Seventy-eight percent of cases showed methylation of at least one gene. No aberrant hypermethylation was detected in control samples. Conclusions: Our study demonstrated the feasibility of detecting multiple molecular tumor markers in NP brushing samples with a high sensitivity and specificity for NPC detection. It offers a powerful yet noninvasive approach for the diagnosis of NPC in high-risk populations.
Persistent Identifierhttp://hdl.handle.net/10722/92235
ISSN
2015 Impact Factor: 8.738
2015 SCImago Journal Rankings: 5.314
References

 

DC FieldValueLanguage
dc.contributor.authorTong, JHMen_HK
dc.contributor.authorTsang, RKYen_HK
dc.contributor.authorLo, KWen_HK
dc.contributor.authorWoo, JKSen_HK
dc.contributor.authorKwong, Jen_HK
dc.contributor.authorChan, MWYen_HK
dc.contributor.authorChang, ARen_HK
dc.contributor.authorVan Hasselt, CAen_HK
dc.contributor.authorHuang, DPen_HK
dc.contributor.authorTo, KFen_HK
dc.date.accessioned2010-09-17T10:40:04Z-
dc.date.available2010-09-17T10:40:04Z-
dc.date.issued2002en_HK
dc.identifier.citationClinical Cancer Research, 2002, v. 8 n. 8, p. 2612-2619en_HK
dc.identifier.issn1078-0432en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92235-
dc.description.abstractPurpose: Nasopharyngeal carcinoma (NPC) is highly prevalent in southern China and characterized by a strong association with EBV. We aimed to detect EBV DNA and cancer-related gene promoter hypermethylation in nasopharyngeal (NP) brushing samples and provide a novel noninvasive approach for NPC detection. Experimental Design: Twenty-eight NPC cases and 26 noncancerous subjects were prospectively recruited. NP brushing samples were subjected to quantitative real-time PCR analysis of EBV DNA and methylation-specific PCR analysis of the DAP-kinase, RASSF1A, and p16 genes. Results: EBV DNA quantity in NP brushing samples from NPC patients (median, 8.94 copies/actin) was significantly higher than that of controls (median, 0 copies/actin; P < 0.0001). Twenty-seven of 28 NPC patients had detectable EBV DNA in NP brushes, whereas 25 of 26 controls had undetectable or very low levels of EBV DNA. Elevated EBV DNA level in brushing samples as a tumor marker had a sensitivity of 96.4% and a specificity of 96.2% for NPC detection. Moreover, T 1 disease had a significantly lower EBV DNA level as compared with locally more advanced disease (P = 0.037). In brushing samples of NPC patients, the frequencies of DAP-kinase, RASSF1A, and p16 promoter hypermethylation were 50.0%, 39.3%, and 46.4%, respectively. Seventy-eight percent of cases showed methylation of at least one gene. No aberrant hypermethylation was detected in control samples. Conclusions: Our study demonstrated the feasibility of detecting multiple molecular tumor markers in NP brushing samples with a high sensitivity and specificity for NPC detection. It offers a powerful yet noninvasive approach for the diagnosis of NPC in high-risk populations.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Researchen_HK
dc.relation.ispartofClinical Cancer Researchen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.titleQuantitative Epstein-Barr virus DNA analysis and detection of gene promoter hypermethylation in nasopharyngeal (NP) brushing samples from patients with NP carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.emailTsang, RKY: rkytsang@hku.hken_HK
dc.identifier.authorityTsang, RKY=rp01386en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0036023426en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036023426&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume8en_HK
dc.identifier.issue8en_HK
dc.identifier.spage2612en_HK
dc.identifier.epage2619en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTong, JHM=7202724564en_HK
dc.identifier.scopusauthoridTsang, RKY=7102940058en_HK
dc.identifier.scopusauthoridLo, KW=34872774800en_HK
dc.identifier.scopusauthoridWoo, JKS=27268094700en_HK
dc.identifier.scopusauthoridKwong, J=7005301973en_HK
dc.identifier.scopusauthoridChan, MWY=7402597788en_HK
dc.identifier.scopusauthoridChang, AR=7402539761en_HK
dc.identifier.scopusauthoridVan Hasselt, CA=7103394173en_HK
dc.identifier.scopusauthoridHuang, DP=7403891486en_HK
dc.identifier.scopusauthoridTo, KF=36785812800en_HK

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