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Article: Expression and clinical significance of chemokine receptor in peripheral blood mononuclear cells in patients with primary biliary cirrhosis

TitleExpression and clinical significance of chemokine receptor in peripheral blood mononuclear cells in patients with primary biliary cirrhosis
Authors
KeywordsBiliary Liver Cirrhosis
Chemokine Receptors
Flow Cytometry
Mononuclear Cells
Issue Date2009
Citation
Academic Journal of Second Military Medical University, 2009, v. 30 n. 2, p. 142-146 How to Cite?
AbstractObjective: To investigate the relationship of chemokine receptor with the development and progression of primary biliary cirrhosis (PBC). Methods: Real-time PCR and flow cytometry were used to examine the mRNA and protein expression of chemokine receptor 1 (CCR1), CCR3 and CCR5 in the peripheral blood mononuclear cells (PBMCs) of 60 patients with PBC, 60 patients with hepatitis B-related cirrhosis, and 60 normal controls. Total bilirubin (TBIL) and γ-glutamyltransferase (γ-GT) levels were determined in the patients with PBC and normal controls,and their correlation with chemotactic factors was also analyzed. Results: Both the mRNA and protein expression levels of CCR1, CCR3 and CCR5 in the PBMCs were significantly lower in PBC patients than those in the other two groups (P< 0.05), and there were no significant differences between the latter two groups. The mRNA and protein levels of CCR1, CCR3 and CCR5 were significantly lower in patients with stage III and IV PBC than those with stage I and II PBC. CCR1 protein expression was correlated with TBIL levels in PBC patients(r= - 0.445,P<0.01), but not with γ-GT(r= - 0.230,P>0.05). CCR3 protein was not linearly correlated with TBIL level (r= -0.173,P>0.05),but was correlated with γ-GT(r= -0.295, P< 0.05). Expression of CCR5 protein was negatively correlated with both TBIL and γ-GT levels(r= -0.531,P<0.01; r=-0.665,P <0.01). Conclusion: CCR1, CCR3 and CCR5 expression is associated with the development and progression of PBC; they may be involved in the regulatory mechanism of PBC,which may cast new lights on the diagnosis and prevention of PBC.
Persistent Identifierhttp://hdl.handle.net/10722/92192
ISSN
2015 SCImago Journal Rankings: 0.105
References

 

DC FieldValueLanguage
dc.contributor.authorHan, Z-Jen_HK
dc.contributor.authorWu, C-Yen_HK
dc.contributor.authorJiang, T-Wen_HK
dc.contributor.authorTang, Y-Jen_HK
dc.contributor.authorZhou, Yen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorGu, M-Len_HK
dc.contributor.authorDeng, A-Men_HK
dc.contributor.authorZhong, R-Qen_HK
dc.date.accessioned2010-09-17T10:38:47Z-
dc.date.available2010-09-17T10:38:47Z-
dc.date.issued2009en_HK
dc.identifier.citationAcademic Journal of Second Military Medical University, 2009, v. 30 n. 2, p. 142-146en_HK
dc.identifier.issn0258-879Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92192-
dc.description.abstractObjective: To investigate the relationship of chemokine receptor with the development and progression of primary biliary cirrhosis (PBC). Methods: Real-time PCR and flow cytometry were used to examine the mRNA and protein expression of chemokine receptor 1 (CCR1), CCR3 and CCR5 in the peripheral blood mononuclear cells (PBMCs) of 60 patients with PBC, 60 patients with hepatitis B-related cirrhosis, and 60 normal controls. Total bilirubin (TBIL) and γ-glutamyltransferase (γ-GT) levels were determined in the patients with PBC and normal controls,and their correlation with chemotactic factors was also analyzed. Results: Both the mRNA and protein expression levels of CCR1, CCR3 and CCR5 in the PBMCs were significantly lower in PBC patients than those in the other two groups (P< 0.05), and there were no significant differences between the latter two groups. The mRNA and protein levels of CCR1, CCR3 and CCR5 were significantly lower in patients with stage III and IV PBC than those with stage I and II PBC. CCR1 protein expression was correlated with TBIL levels in PBC patients(r= - 0.445,P<0.01), but not with γ-GT(r= - 0.230,P>0.05). CCR3 protein was not linearly correlated with TBIL level (r= -0.173,P>0.05),but was correlated with γ-GT(r= -0.295, P< 0.05). Expression of CCR5 protein was negatively correlated with both TBIL and γ-GT levels(r= -0.531,P<0.01; r=-0.665,P <0.01). Conclusion: CCR1, CCR3 and CCR5 expression is associated with the development and progression of PBC; they may be involved in the regulatory mechanism of PBC,which may cast new lights on the diagnosis and prevention of PBC.en_HK
dc.languageengen_HK
dc.relation.ispartofAcademic Journal of Second Military Medical Universityen_HK
dc.subjectBiliary Liver Cirrhosisen_HK
dc.subjectChemokine Receptorsen_HK
dc.subjectFlow Cytometryen_HK
dc.subjectMononuclear Cellsen_HK
dc.titleExpression and clinical significance of chemokine receptor in peripheral blood mononuclear cells in patients with primary biliary cirrhosisen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, Y:ychenc@hkucc.hku.hken_HK
dc.identifier.authorityChen, Y=rp1318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3724/SP.J.1008.2009.00142en_HK
dc.identifier.scopuseid_2-s2.0-61449229392en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-61449229392&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue2en_HK
dc.identifier.spage142en_HK
dc.identifier.epage146en_HK

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