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Article: CDNA cloning and expression of autoantigen B0-E2

TitleCDNA cloning and expression of autoantigen B0-E2
Authors
KeywordsBcoadc-E2
Bo-E2 Recombinant Fusion Protein
Ogdc-E2
Primary Biliary Cirrhosis
Western Blotting
Issue Date2007
Publisher中國中西醫結合學會. The Journal's web site is located at http://xxhb.chinajournal.net.cn/
Citation
World Chinese Journal of Digestology, 2007, v. 15 n. 22, p. 2465-2467 How to Cite?
AbstractAIM: To express BO-E2 recombinant fusion protein in Escherichia coli for use in early diagnosis of primary biliary cirrhosis (PBC). METHODS: CDNA encoding BCOADC-E2 and OGDC-E2 were obtained by reverse transcription pdymerase chain reaction (RT-PCR), confirmed by DNA sequencing, subcloned non-directionally into the bacterial expression plasmid pET28a(+), and then transformed into E. coli BL21(DE3) to express the recombinant fusion protein, which was identified by SDS-PAGE and western blotting. RESULTS: Results of nucleotide sequencing and restriction analysis showed that the recombinant plasmid successfully constructed three species. Three types of fusion protein were expressed by isopropyl-Beta-d-thiogalactopyranoside (IPTG) induction. The recombinant fusion protein exhibited the antigenic-ity of AMA-M2 by western blotting. CONCLUSION: BO-E2 recombinant fusion protein can be used for in vitro diagnosis of PBC.
Persistent Identifierhttp://hdl.handle.net/10722/92168
ISSN
2015 SCImago Journal Rankings: 0.104
References

 

DC FieldValueLanguage
dc.contributor.authorZhou, Yen_HK
dc.contributor.authorYao, D-Ken_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorJiang, T-Sen_HK
dc.contributor.authorChen, Ben_HK
dc.contributor.authorZhong, R-Qen_HK
dc.contributor.authorDeng, A-Men_HK
dc.date.accessioned2010-09-17T10:38:04Z-
dc.date.available2010-09-17T10:38:04Z-
dc.date.issued2007en_HK
dc.identifier.citationWorld Chinese Journal of Digestology, 2007, v. 15 n. 22, p. 2465-2467en_HK
dc.identifier.issn1009-3079en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92168-
dc.description.abstractAIM: To express BO-E2 recombinant fusion protein in Escherichia coli for use in early diagnosis of primary biliary cirrhosis (PBC). METHODS: CDNA encoding BCOADC-E2 and OGDC-E2 were obtained by reverse transcription pdymerase chain reaction (RT-PCR), confirmed by DNA sequencing, subcloned non-directionally into the bacterial expression plasmid pET28a(+), and then transformed into E. coli BL21(DE3) to express the recombinant fusion protein, which was identified by SDS-PAGE and western blotting. RESULTS: Results of nucleotide sequencing and restriction analysis showed that the recombinant plasmid successfully constructed three species. Three types of fusion protein were expressed by isopropyl-Beta-d-thiogalactopyranoside (IPTG) induction. The recombinant fusion protein exhibited the antigenic-ity of AMA-M2 by western blotting. CONCLUSION: BO-E2 recombinant fusion protein can be used for in vitro diagnosis of PBC.en_HK
dc.languageengen_HK
dc.publisher中國中西醫結合學會. The Journal's web site is located at http://xxhb.chinajournal.net.cn/zh_HK
dc.relation.ispartofWorld Chinese Journal of Digestologyen_HK
dc.subjectBcoadc-E2en_HK
dc.subjectBo-E2 Recombinant Fusion Proteinen_HK
dc.subjectOgdc-E2en_HK
dc.subjectPrimary Biliary Cirrhosisen_HK
dc.subjectWestern Blottingen_HK
dc.titleCDNA cloning and expression of autoantigen B0-E2en_HK
dc.typeArticleen_HK
dc.identifier.emailChen, Y:ychenc@hkucc.hku.hken_HK
dc.identifier.authorityChen, Y=rp1318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-36048970808en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-36048970808&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume15en_HK
dc.identifier.issue22en_HK
dc.identifier.spage2465en_HK
dc.identifier.epage2467en_HK

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