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Article: Characterization of tissue-specific LIM domain protein (FHL1C) which is an alternatively spliced isoform of a human LIM-only protein (FHL1)

TitleCharacterization of tissue-specific LIM domain protein (FHL1C) which is an alternatively spliced isoform of a human LIM-only protein (FHL1)
Authors
KeywordsFHL1
FHL1C
KyoT2
RBP-J
SLIM1
Zinc finger protein
Issue Date2001
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503
Citation
Journal Of Cellular Biochemistry, 2001, v. 82 n. 1, p. 1-10 How to Cite?
AbstractWe have cloned and characterized another alternatively spliced isoform of the human four-and-a-half LIM domain protein 1 (FHL1), designated FHL1C. FHL1C contains a single zinc finger and two tandem repeats of LIM domains at the N-terminus followed by a putative RBP-J binding region at the C-terminus. FHL1C shares the same N-terminal two-and-a-half LIM domains with FHL1 but different C-terminal protein sequences. Due to the absence of the exon 4 in FHL1 C, there is a frame-shift in the 3′ coding region. Sequence analysis indicated that FHL1C is the human homolog of murine KyoT2. The Northern blot and RT-PCR results revealed that FHL1 is widely expressed in human tissues, including skeletal muscle and heart at a high level, albeit as a relatively low abundance transcript in brain, placenta, lung, liver, kidney, pancreas, and testis. In contrast, FHL1C is specifically expressed in testis, skeletal muscle, and heart at a relatively low level compared with FHL1. The expression of FHL1C transcripts was also seen in aorta, left atrium, left, and right ventricles of human heart at low level. Immunoblot analysis using affinity-purified anti-FHL1C antipeptide antibodies confirmed a 20 kDa protein of FHL1C in human skeletal muscle and heart. Unlike FHL1 B, which is another FHL1 isoform recently reported by our group and localized predominantly in the nucleus [Lee et al., 1999], FHL1C is localized both in the nucleus and cytoplasm of mammalian cell. © 2001 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/92157
ISSN
2014 Impact Factor: 3.263
2014 SCImago Journal Rankings: 1.275
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNg, EKOen_HK
dc.contributor.authorLee, SMYen_HK
dc.contributor.authorLi, HYen_HK
dc.contributor.authorNgai, SMen_HK
dc.contributor.authorTsui, SKWen_HK
dc.contributor.authorWaye, MMYen_HK
dc.contributor.authorLee, CYen_HK
dc.contributor.authorFung, KPen_HK
dc.date.accessioned2010-09-17T10:37:45Z-
dc.date.available2010-09-17T10:37:45Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Cellular Biochemistry, 2001, v. 82 n. 1, p. 1-10en_HK
dc.identifier.issn0730-2312en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92157-
dc.description.abstractWe have cloned and characterized another alternatively spliced isoform of the human four-and-a-half LIM domain protein 1 (FHL1), designated FHL1C. FHL1C contains a single zinc finger and two tandem repeats of LIM domains at the N-terminus followed by a putative RBP-J binding region at the C-terminus. FHL1C shares the same N-terminal two-and-a-half LIM domains with FHL1 but different C-terminal protein sequences. Due to the absence of the exon 4 in FHL1 C, there is a frame-shift in the 3′ coding region. Sequence analysis indicated that FHL1C is the human homolog of murine KyoT2. The Northern blot and RT-PCR results revealed that FHL1 is widely expressed in human tissues, including skeletal muscle and heart at a high level, albeit as a relatively low abundance transcript in brain, placenta, lung, liver, kidney, pancreas, and testis. In contrast, FHL1C is specifically expressed in testis, skeletal muscle, and heart at a relatively low level compared with FHL1. The expression of FHL1C transcripts was also seen in aorta, left atrium, left, and right ventricles of human heart at low level. Immunoblot analysis using affinity-purified anti-FHL1C antipeptide antibodies confirmed a 20 kDa protein of FHL1C in human skeletal muscle and heart. Unlike FHL1 B, which is another FHL1 isoform recently reported by our group and localized predominantly in the nucleus [Lee et al., 1999], FHL1C is localized both in the nucleus and cytoplasm of mammalian cell. © 2001 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503en_HK
dc.relation.ispartofJournal of Cellular Biochemistryen_HK
dc.subjectFHL1en_HK
dc.subjectFHL1Cen_HK
dc.subjectKyoT2en_HK
dc.subjectRBP-Jen_HK
dc.subjectSLIM1en_HK
dc.subjectZinc finger proteinen_HK
dc.titleCharacterization of tissue-specific LIM domain protein (FHL1C) which is an alternatively spliced isoform of a human LIM-only protein (FHL1)en_HK
dc.typeArticleen_HK
dc.identifier.emailNg, EKO: ngko@hku.hken_HK
dc.identifier.authorityNg, EKO=rp01364en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jcb.1110en_HK
dc.identifier.pmid11400158-
dc.identifier.scopuseid_2-s2.0-0034994307en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034994307&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume82en_HK
dc.identifier.issue1en_HK
dc.identifier.spage1en_HK
dc.identifier.epage10en_HK
dc.identifier.isiWOS:000169049400001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridNg, EKO=21135553700en_HK
dc.identifier.scopusauthoridLee, SMY=35233892600en_HK
dc.identifier.scopusauthoridLi, HY=12762326800en_HK
dc.identifier.scopusauthoridNgai, SM=7006074219en_HK
dc.identifier.scopusauthoridTsui, SKW=7004961364en_HK
dc.identifier.scopusauthoridWaye, MMY=7006687733en_HK
dc.identifier.scopusauthoridLee, CY=7410142857en_HK
dc.identifier.scopusauthoridFung, KP=35271657800en_HK

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