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Article: Effects of curcumin on the acetylation of histone H3, P53 and the proliferation of NB4 cells

TitleEffects of curcumin on the acetylation of histone H3, P53 and the proliferation of NB4 cells
Authors
KeywordsCurcumin (姜黃素)
Histone H3 (組蛋白H3)
Protein, P53 (蛋白質,P53)
Cell line, NB4 (細胞系,NB4)
Issue Date2005
PublisherZhonghua yi xue hui (中華醫學會)
Citation
Chinese journal of hematology, 2005, v. 26 n. 9, p. 551-553 How to Cite?
中華血液學雜誌, 2005, v. 26 n. 9, p. 551-553 How to Cite?
AbstractOBJECTIVE: To investigate the effects of curcumin on the acetylation of histone H3, P53 and the proliferation of NB4 cells. METHODS: The total protein was extracted from NB4 cells treated without or with different concentrations of curcumin ( 50, 25, 12.5, 6.25 and 3.125 micromol/L) for different time (0, 4, 8, 12, 24 h). Western blot analysis was performed to determine the levels of acetylated histone H3, P53 and acetylated P53. MTT assay was performed to examine the growth inhibition effect of curcumin on NB4 cells. RESULTS: Curcumin could inhibit the proliferation of NB4 cells in a time- and dose- dependent manner, with the IC50 at 24 h and 36 h of 40 micromol/L and 25 micromol/L, respectively. The levels of histone H3 acetylation, P53 expression and P53 acetylation were increased obviously. CONCLUSION: Curcumin functions as a deacetylase inhibitor,which could increase the level of acetylated histone H3, enhance the expression and activity of tumor suppressor P53, and inhibit the proliferation of NB4 cells.
目的研究姜黃素對NB4細胞組蛋白H3和非組蛋白P53的乙酰化和細胞增殖的作用,探討姜黃素抗白血病機制。方法應用不同濃度(50,25,12.5,6.25,3.125μmol/L)的姜黃素作用NB4細胞不同時間(0,4,8,12,24h),MTT法測定姜黃素對NB4細胞增殖的影響,Western blot法檢測乙酰化組蛋白H3和乙酰化P53的水平。結果姜黃素以時間和劑量依賴方式抑制NB4細胞增殖,在24h和36h的IC50值分別為40μmol/L和25μmol/L;能明顯上調組蛋白H3的乙酰化水平,促進P53的表達和P53的乙酰化。結論姜黃素具有去乙酰化酶抑制劑作用,能上調組蛋白H3乙酰化水平,促進腫瘤抑制因子P53表達和活化,抑制白血病細胞增殖。
Persistent Identifierhttp://hdl.handle.net/10722/92140
ISSN
2015 SCImago Journal Rankings: 0.137

 

DC FieldValueLanguage
dc.contributor.authorLi, XGen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorWu, Qen_HK
dc.contributor.authorLiu, HLen_HK
dc.date.accessioned2010-09-17T10:37:15Z-
dc.date.available2010-09-17T10:37:15Z-
dc.date.issued2005en_HK
dc.identifier.citationChinese journal of hematology, 2005, v. 26 n. 9, p. 551-553en_HK
dc.identifier.citation中華血液學雜誌, 2005, v. 26 n. 9, p. 551-553-
dc.identifier.issn0253-2727en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92140-
dc.description.abstractOBJECTIVE: To investigate the effects of curcumin on the acetylation of histone H3, P53 and the proliferation of NB4 cells. METHODS: The total protein was extracted from NB4 cells treated without or with different concentrations of curcumin ( 50, 25, 12.5, 6.25 and 3.125 micromol/L) for different time (0, 4, 8, 12, 24 h). Western blot analysis was performed to determine the levels of acetylated histone H3, P53 and acetylated P53. MTT assay was performed to examine the growth inhibition effect of curcumin on NB4 cells. RESULTS: Curcumin could inhibit the proliferation of NB4 cells in a time- and dose- dependent manner, with the IC50 at 24 h and 36 h of 40 micromol/L and 25 micromol/L, respectively. The levels of histone H3 acetylation, P53 expression and P53 acetylation were increased obviously. CONCLUSION: Curcumin functions as a deacetylase inhibitor,which could increase the level of acetylated histone H3, enhance the expression and activity of tumor suppressor P53, and inhibit the proliferation of NB4 cells.en_HK
dc.description.abstract目的研究姜黃素對NB4細胞組蛋白H3和非組蛋白P53的乙酰化和細胞增殖的作用,探討姜黃素抗白血病機制。方法應用不同濃度(50,25,12.5,6.25,3.125μmol/L)的姜黃素作用NB4細胞不同時間(0,4,8,12,24h),MTT法測定姜黃素對NB4細胞增殖的影響,Western blot法檢測乙酰化組蛋白H3和乙酰化P53的水平。結果姜黃素以時間和劑量依賴方式抑制NB4細胞增殖,在24h和36h的IC50值分別為40μmol/L和25μmol/L;能明顯上調組蛋白H3的乙酰化水平,促進P53的表達和P53的乙酰化。結論姜黃素具有去乙酰化酶抑制劑作用,能上調組蛋白H3乙酰化水平,促進腫瘤抑制因子P53表達和活化,抑制白血病細胞增殖。-
dc.languagechien_HK
dc.publisherZhonghua yi xue hui (中華醫學會)-
dc.relation.ispartofChinese journal of hematologyen_HK
dc.relation.ispartof中華血液學雜誌-
dc.subjectCurcumin (姜黃素)en_HK
dc.subjectHistone H3 (組蛋白H3)-
dc.subjectProtein, P53 (蛋白質,P53)-
dc.subjectCell line, NB4 (細胞系,NB4)-
dc.titleEffects of curcumin on the acetylation of histone H3, P53 and the proliferation of NB4 cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, Y:ychenc@hkucc.hku.hken_HK
dc.identifier.authorityChen, Y=rp1318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid16468334-
dc.identifier.scopuseid_2-s2.0-66049135144en_HK
dc.identifier.volume26en_HK
dc.identifier.issue9en_HK
dc.identifier.spage551en_HK
dc.identifier.epage553en_HK
dc.publisher.placeChina (中國)-
dc.customcontrol.immutablecsl 150112-

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