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Article: Molecular cloning of grass carp growth hormone receptor and its functional interaction with silver carp growth hormone

TitleMolecular cloning of grass carp growth hormone receptor and its functional interaction with silver carp growth hormone
Authors
Hu, WChen, XYu, GChen, YShen, YZhang, YGong, Y
KeywordsSpecies Index: Ctenopharyngodon
Cyprinidae
Eukaryota
Hypophthalmichthys Nobilis
Issue Date2009
PublisherBentham Science Publishers Ltd. The Journal's web site is located at http://www.bentham.org/ppl/index.htm
Citation
Protein and Peptide Letters, 2009, v. 16 n. 3, p. 320-323 How to Cite?
DOI: http://dx.doi.org/10.2174/092986609787601679
AbstractA novel grass carp growth hormone receptor (gcGHR) cDNA was cloned by RT-PCR method. CHO-K1 cells were transfected with eukaryotic expression vector pcDNA3.0 containing gcGHR entire coding region, resulting in a stable CHO cell line expressing gcGHR (CHO-gcGHR). Bioactivity assay revealed that silver carp growth hormone (scGH) bound gcGHR with dissociation constant (Kd) value of 1.96 nM and stimulated CHO-gcGHR cells proliferation with the sensitivity of 20 ng/ml. The cells proliferation assay was also provided to measure fish growth hormone levels of pituitary and refolded protein. © 2009 Bentham Science Publishers Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/92122
ISSN
0929-8665
2023 Impact Factor: 1.0
2023 SCImago Journal Rankings: 0.349
ISI Accession Number ID
WOS:000263980400016
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorHu, Wen_HK
dc.contributor.authorChen, Xen_HK
dc.contributor.authorYu, Gen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorShen, Yen_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorGong, Yen_HK
dc.date.accessioned2010-09-17T10:36:44Z-
dc.date.available2010-09-17T10:36:44Z-
dc.date.issued2009en_HK
dc.identifier.citationProtein and Peptide Letters, 2009, v. 16 n. 3, p. 320-323en_HK
dc.identifier.issn0929-8665en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92122-
dc.description.abstractA novel grass carp growth hormone receptor (gcGHR) cDNA was cloned by RT-PCR method. CHO-K1 cells were transfected with eukaryotic expression vector pcDNA3.0 containing gcGHR entire coding region, resulting in a stable CHO cell line expressing gcGHR (CHO-gcGHR). Bioactivity assay revealed that silver carp growth hormone (scGH) bound gcGHR with dissociation constant (Kd) value of 1.96 nM and stimulated CHO-gcGHR cells proliferation with the sensitivity of 20 ng/ml. The cells proliferation assay was also provided to measure fish growth hormone levels of pituitary and refolded protein. © 2009 Bentham Science Publishers Ltd.en_HK
dc.languageengen_HK
dc.publisherBentham Science Publishers Ltd. The Journal's web site is located at http://www.bentham.org/ppl/index.htmen_HK
dc.relation.ispartofProtein and Peptide Lettersen_HK
dc.subjectSpecies Index: Ctenopharyngodonen_HK
dc.subjectCyprinidaeen_HK
dc.subjectEukaryotaen_HK
dc.subjectHypophthalmichthys Nobilisen_HK
dc.titleMolecular cloning of grass carp growth hormone receptor and its functional interaction with silver carp growth hormoneen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, Y:ychenc@hkucc.hku.hken_HK
dc.identifier.authorityChen, Y=rp1318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.2174/092986609787601679en_HK
dc.identifier.pmid19275748-
dc.identifier.scopuseid_2-s2.0-63449101563en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-63449101563&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume16en_HK
dc.identifier.issue3en_HK
dc.identifier.spage320en_HK
dc.identifier.epage323en_HK
dc.identifier.isiWOS:000263980400016-
dc.identifier.citeulike4145824-
dc.identifier.issnl0929-8665-

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