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Article: Effect of Gambogic acid on the regulation of hERG channel in K562 cells in vitro

TitleEffect of Gambogic acid on the regulation of hERG channel in K562 cells in vitro
Authors
KeywordsApoptosis
Gambogic Acid
Herg
Leukemia
Issue Date2009
Citation
Journal of Huazhong University of Science and Technology - Medical Science, 2009, v. 29 n. 5, p. 540-545 How to Cite?
AbstractOverexpression of human ether-à-go-go (eag) related gene (hERG) has been found in a broad range of human leukemia cell lines and primary human leukemia. The block of hERG protein might be a potential therapeutic strategy for leukemia. Gambogic acid (GA) has recently exhibited marked anti-tumor potency on solid tumors of various derivations. Here, we investigated the anti-leukemia effects of GA and its relation to the regulation of hERG in K562 leukemia cells in vitro. K562 cells were treated with various concentrations of GA (0.125-8.0 μmol/L) for 0-72 h. MTT assay was used to evaluate the inhibition effect of GA on the growth of K562 cells. Cell apoptosis was measured through both Annexin-V FITC/PI double-labeled cytometry and transmission electron microscopy. Cell cycle regulation was studied by a propidium iodide method. RT-PCR and Western blot were applied to detect the expression level of hERG in K562 cells. GA presented striking growth inhibition and apoptosis induction potency on K562 cells in vitro in a time- and dose-dependent manner. The IC50 value of GA for 24 h was 2.637±0.208 μmol/L. Moreover, GA induced K562 cells arrested in G0/G1 phase, accordingly, cells in S phase decreased gradually, and no obvious changes were found in G2/M phase cells. Under the transmission electron microscopy, apoptotic bodies containing nuclear fragments were found in GA-treated K562 cells. After treatment with GA of 2.0 μmol/L for 24 h, the percentage of apoptotic cells was increased from 4.09% to 18.47% (P<0.01). Overexpression of hERG channel was found in K562 cells, while GA could down-regulate it at both protein and mRNA levels (P<0.01). It was concluded that GA exhibited its anti-leukemia effects partially through down-regulating the expression level of hERG channel in K562 cells, suggesting that GA may be a potential agent against leukemia with a mechanism of blocking hERG channel. © 2009 Huazhong University of Science and Technology and Springer Berlin Heidelberg.
Persistent Identifierhttp://hdl.handle.net/10722/92105
ISSN
2015 Impact Factor: 0.838
2015 SCImago Journal Rankings: 0.344
ISI Accession Number ID
Funding AgencyGrant Number
National Nature Sciences Foundation of China30472267
Funding Information:

This project was supported by a grant from the National Nature Sciences Foundation of China ( No. 30472267).

References

 

DC FieldValueLanguage
dc.contributor.authorCui, Gen_HK
dc.contributor.authorShu, Wen_HK
dc.contributor.authorWu, Qen_HK
dc.contributor.authorChen, Yen_HK
dc.date.accessioned2010-09-17T10:36:13Z-
dc.date.available2010-09-17T10:36:13Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal of Huazhong University of Science and Technology - Medical Science, 2009, v. 29 n. 5, p. 540-545en_HK
dc.identifier.issn1672-0733en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92105-
dc.description.abstractOverexpression of human ether-à-go-go (eag) related gene (hERG) has been found in a broad range of human leukemia cell lines and primary human leukemia. The block of hERG protein might be a potential therapeutic strategy for leukemia. Gambogic acid (GA) has recently exhibited marked anti-tumor potency on solid tumors of various derivations. Here, we investigated the anti-leukemia effects of GA and its relation to the regulation of hERG in K562 leukemia cells in vitro. K562 cells were treated with various concentrations of GA (0.125-8.0 μmol/L) for 0-72 h. MTT assay was used to evaluate the inhibition effect of GA on the growth of K562 cells. Cell apoptosis was measured through both Annexin-V FITC/PI double-labeled cytometry and transmission electron microscopy. Cell cycle regulation was studied by a propidium iodide method. RT-PCR and Western blot were applied to detect the expression level of hERG in K562 cells. GA presented striking growth inhibition and apoptosis induction potency on K562 cells in vitro in a time- and dose-dependent manner. The IC50 value of GA for 24 h was 2.637±0.208 μmol/L. Moreover, GA induced K562 cells arrested in G0/G1 phase, accordingly, cells in S phase decreased gradually, and no obvious changes were found in G2/M phase cells. Under the transmission electron microscopy, apoptotic bodies containing nuclear fragments were found in GA-treated K562 cells. After treatment with GA of 2.0 μmol/L for 24 h, the percentage of apoptotic cells was increased from 4.09% to 18.47% (P<0.01). Overexpression of hERG channel was found in K562 cells, while GA could down-regulate it at both protein and mRNA levels (P<0.01). It was concluded that GA exhibited its anti-leukemia effects partially through down-regulating the expression level of hERG channel in K562 cells, suggesting that GA may be a potential agent against leukemia with a mechanism of blocking hERG channel. © 2009 Huazhong University of Science and Technology and Springer Berlin Heidelberg.en_HK
dc.languageengen_HK
dc.relation.ispartofJournal of Huazhong University of Science and Technology - Medical Scienceen_HK
dc.subjectApoptosisen_HK
dc.subjectGambogic Aciden_HK
dc.subjectHergen_HK
dc.subjectLeukemiaen_HK
dc.titleEffect of Gambogic acid on the regulation of hERG channel in K562 cells in vitroen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, Y:ychenc@hkucc.hku.hken_HK
dc.identifier.authorityChen, Y=rp1318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s11596-009-0503-8en_HK
dc.identifier.pmid19821083-
dc.identifier.scopuseid_2-s2.0-70350651413en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70350651413&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue5en_HK
dc.identifier.spage540en_HK
dc.identifier.epage545en_HK
dc.identifier.isiWOS:000270681700003-
dc.identifier.citeulike6446246-

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