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Article: Deguelin regulates cell cycle and nuclear pore complex protein Nup98 and Nup88 in U937 cells in vitro

TitleDeguelin regulates cell cycle and nuclear pore complex protein Nup98 and Nup88 in U937 cells in vitro
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2007
Citation
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi, 2007, v. 28 n. 2, p. 115-118 How to Cite?
AbstractOBJECTIVE: To investigate antitumor activity and molecular mechanism of deguelin to the human U937 leukaemia cells and to explore the mechanisms regulating cell cycle and nucleoporin 98 (Nup98) and nucleoporin 88 (Nup88) in vitro. METHODS: The effects of deguelin on the growth of U937 cells were studied by MTT assay, and the cell cycle of U937 cells by a propidium iodide method. The localization of the nuclear pore complex protein Nup98 and Nup88 was checked by immunofluorescence and immunoelectron microscopy. The expressions of Nup98 and Nup88 in U937 cells were checked by flow cytometry (FCM) and Western blot respectively. RESULTS: The proliferation of U937 cells was significantly inhibited in a time-dose dependent manner in deguelin-treated group with a 24 h IC50 value of 21.61 nmol/L and 36 h IC50 value of 17.07 nmol/L. U937 cells treated with deguelin showed reduction in the percentages of cells in G0/G1, whereas accumulation of cells in S and G2/M phase. The ratio of G1/G0 phase cells were 73.01%, 71.15%, 68.42%, 52.45%, 43.99% and 22.82%, and that of S phase cells were 17.18%, 16.30%, 18.09%, 27.56%, 31.21% and 46.85%, and that of G2/M phase cells were 9.75%, 12.31%, 13.09%, 18.99%, 24.83% and 27.79% at deguelin concentrations of 0, 5, 10, 20, 40, 80 nmol/L respectively. Nup88 and Nup98 were found on both the nuclear and cytoplasmic side of the U937 cells. The expression of Nup98 was up-regulated and Nup88 down-regulated in deguelin treated U937 cells. CONCLUSION: Deguelin is able to inhibit the proliferation of U937 cells by regulating the cell cycle. The antitumor activity of deguelin was related to up-regulating the expression of Nup98 and down-regulating Nup88 protein.
Persistent Identifierhttp://hdl.handle.net/10722/92079
ISSN
2015 SCImago Journal Rankings: 0.137

 

DC FieldValueLanguage
dc.contributor.authorChen, Yen_HK
dc.contributor.authorLiu, HLen_HK
dc.contributor.authorCui, GHen_HK
dc.contributor.authorWu, QLen_HK
dc.contributor.authorHe, Jen_HK
dc.contributor.authorChen, WHen_HK
dc.date.accessioned2010-09-17T10:35:28Z-
dc.date.available2010-09-17T10:35:28Z-
dc.date.issued2007en_HK
dc.identifier.citationZhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi, 2007, v. 28 n. 2, p. 115-118en_HK
dc.identifier.issn0253-2727en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92079-
dc.description.abstractOBJECTIVE: To investigate antitumor activity and molecular mechanism of deguelin to the human U937 leukaemia cells and to explore the mechanisms regulating cell cycle and nucleoporin 98 (Nup98) and nucleoporin 88 (Nup88) in vitro. METHODS: The effects of deguelin on the growth of U937 cells were studied by MTT assay, and the cell cycle of U937 cells by a propidium iodide method. The localization of the nuclear pore complex protein Nup98 and Nup88 was checked by immunofluorescence and immunoelectron microscopy. The expressions of Nup98 and Nup88 in U937 cells were checked by flow cytometry (FCM) and Western blot respectively. RESULTS: The proliferation of U937 cells was significantly inhibited in a time-dose dependent manner in deguelin-treated group with a 24 h IC50 value of 21.61 nmol/L and 36 h IC50 value of 17.07 nmol/L. U937 cells treated with deguelin showed reduction in the percentages of cells in G0/G1, whereas accumulation of cells in S and G2/M phase. The ratio of G1/G0 phase cells were 73.01%, 71.15%, 68.42%, 52.45%, 43.99% and 22.82%, and that of S phase cells were 17.18%, 16.30%, 18.09%, 27.56%, 31.21% and 46.85%, and that of G2/M phase cells were 9.75%, 12.31%, 13.09%, 18.99%, 24.83% and 27.79% at deguelin concentrations of 0, 5, 10, 20, 40, 80 nmol/L respectively. Nup88 and Nup98 were found on both the nuclear and cytoplasmic side of the U937 cells. The expression of Nup98 was up-regulated and Nup88 down-regulated in deguelin treated U937 cells. CONCLUSION: Deguelin is able to inhibit the proliferation of U937 cells by regulating the cell cycle. The antitumor activity of deguelin was related to up-regulating the expression of Nup98 and down-regulating Nup88 protein.en_HK
dc.languageengen_HK
dc.relation.ispartofZhonghua xue ye xue za zhi = Zhonghua xueyexue zazhien_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.titleDeguelin regulates cell cycle and nuclear pore complex protein Nup98 and Nup88 in U937 cells in vitroen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, Y:ychenc@hkucc.hku.hken_HK
dc.identifier.authorityChen, Y=rp1318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid17650673-
dc.identifier.scopuseid_2-s2.0-67651159343en_HK
dc.identifier.volume28en_HK
dc.identifier.issue2en_HK
dc.identifier.spage115en_HK
dc.identifier.epage118en_HK

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