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Article: Regulation of gambogic acid on hERG potassium channel protein of K562 leukemia cells

TitleRegulation of gambogic acid on hERG potassium channel protein of K562 leukemia cells
Authors
KeywordsGambogic Acid (Ga)
Human Ether-À-Go-Go Related Genes (Hkrg)
K562 Cell Apoptosis
Leukemia
Issue Date2009
Citation
Chinese Traditional and Herbal Drugs, 2009, v. 40 n. 6, p. 915-919 How to Cite?
AbstractObjective: To explore the effects of gambogic acid (GA) on proliferation, apoptosis, and cell cycle distribution of leukemia cells K562, as well as to observe its regulation on the expression of human ether-à-go-go related genes (hKRG) protein. Methods: K562 Cells were treated with various concentration of GA (0. 125 - 8. 0 μmol/L) for 0 - 72 h. MTT Assay was used to evalute the inhibition of GA on the growth of K562 cells. Cell apoptosis was detected through both Annexin-V/P1 double-labeled cytometry and transmission electron microscopy (TEC). Cell cycle regulation was studied by a propidium iodide method. Western blotting and RT-PCR technologies were applied to assess the regulation on the expression level of hKRG in K562 cells. Results: GA presented striking growth inhibition on the proliferation of K562 cells in vitro in a time- and dose-dependent manner. The IC50 value of GA for 24 h was (2. 637 ± 0. 208) μmol/L. Moreover, GA induced K562 cells apoptosis in a dose-dependent manner and companied with the obvious morphological changes of apoptosis. Meanwhile, the apoptosis induction potency of CA on K562 cells might correlate well with its effect on the G 0/G1 cell cycle arrest. Overexpression of hKRG potassium channel protein was found in K562 cells, while GA could downregulate it at both protein and mRNA level in a dose-dependent manner (P < 0. 01). Conclusion: CA could exhibit its anti-leukemia effects partially through the downregulation of the protein expression level of hERG potassium channel in K562 cells, which suggests that hKRG potassium channel might be a new target for future therapy of leukemia.
Persistent Identifierhttp://hdl.handle.net/10722/92038
ISSN
2015 SCImago Journal Rankings: 0.130
References

 

DC FieldValueLanguage
dc.contributor.authorCui, G-Hen_HK
dc.contributor.authorShu, W-Xen_HK
dc.contributor.authorWu, Qen_HK
dc.contributor.authorChen, Yen_HK
dc.date.accessioned2010-09-17T10:34:15Z-
dc.date.available2010-09-17T10:34:15Z-
dc.date.issued2009en_HK
dc.identifier.citationChinese Traditional and Herbal Drugs, 2009, v. 40 n. 6, p. 915-919en_HK
dc.identifier.issn0253-2670en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92038-
dc.description.abstractObjective: To explore the effects of gambogic acid (GA) on proliferation, apoptosis, and cell cycle distribution of leukemia cells K562, as well as to observe its regulation on the expression of human ether-à-go-go related genes (hKRG) protein. Methods: K562 Cells were treated with various concentration of GA (0. 125 - 8. 0 μmol/L) for 0 - 72 h. MTT Assay was used to evalute the inhibition of GA on the growth of K562 cells. Cell apoptosis was detected through both Annexin-V/P1 double-labeled cytometry and transmission electron microscopy (TEC). Cell cycle regulation was studied by a propidium iodide method. Western blotting and RT-PCR technologies were applied to assess the regulation on the expression level of hKRG in K562 cells. Results: GA presented striking growth inhibition on the proliferation of K562 cells in vitro in a time- and dose-dependent manner. The IC50 value of GA for 24 h was (2. 637 ± 0. 208) μmol/L. Moreover, GA induced K562 cells apoptosis in a dose-dependent manner and companied with the obvious morphological changes of apoptosis. Meanwhile, the apoptosis induction potency of CA on K562 cells might correlate well with its effect on the G 0/G1 cell cycle arrest. Overexpression of hKRG potassium channel protein was found in K562 cells, while GA could downregulate it at both protein and mRNA level in a dose-dependent manner (P < 0. 01). Conclusion: CA could exhibit its anti-leukemia effects partially through the downregulation of the protein expression level of hERG potassium channel in K562 cells, which suggests that hKRG potassium channel might be a new target for future therapy of leukemia.en_HK
dc.languageengen_HK
dc.relation.ispartofChinese Traditional and Herbal Drugsen_HK
dc.subjectGambogic Acid (Ga)en_HK
dc.subjectHuman Ether-À-Go-Go Related Genes (Hkrg)en_HK
dc.subjectK562 Cell Apoptosisen_HK
dc.subjectLeukemiaen_HK
dc.titleRegulation of gambogic acid on hERG potassium channel protein of K562 leukemia cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, Y:ychenc@hkucc.hku.hken_HK
dc.identifier.authorityChen, Y=rp1318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-68849097087en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-68849097087&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume40en_HK
dc.identifier.issue6en_HK
dc.identifier.spage915en_HK
dc.identifier.epage919en_HK

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