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Article: Effect on proliferation and apoptosis in RPM1-8226 human myeloma cells and regulation on steroid receptor coactivator-3 by deguelin

TitleEffect on proliferation and apoptosis in RPM1-8226 human myeloma cells and regulation on steroid receptor coactivator-3 by deguelin
Authors
KeywordsApoptosis
Deguelin
Multiple Myeloma
Steroid Receptor Coactivator-3 (Src-3)
Issue Date2009
Citation
Chinese Traditional and Herbal Drugs, 2009, v. 40 n. 7, p. 1086-1090 How to Cite?
AbstractObjective: To investigate the effects of deguelin on the proliferation inhibition and apoptosis induction in RPMI-8226 cells in vitro, as well as the regulation of steroid receptor coactivator-3 (SRC-3) to explore the relationship between them. Methods: The effect of deguelin on the growth of RPMI-8226 cells was studied by MTT assay. Apoptosis was detected through Hoechst 33258 staining and Annexin-V FITC/PI double-labeled cytometry. RT-PCR Technology was applied to assessment of the mRNA expression of SRC-3, whereas, SRC-3 protein expression and localization were determined by using immunohistochemistry method. Results: Deguelin presented striking proliferation inhibition potency on RPMI-8226 cells in vitro and apoptosis induction activity in a time- and dose-dependent manner. The IC50 value for 24 h was (54.55±0.40) nmol/L, (17.04±0.73)% RPMI-8226 cells went apoptotic when treated with 25 nmol/L deguelin for 24 h, with the dose of deguelin increasing to 100 nmol/L, (63.57±1.36)% cells were apoptotic. Over-expression of SRC-3 was found in RPMI-8226 cells, whereas the mRNA and protein expression levels of SRC-3 were significantly downregulated in RPMI-8226 cells induced by deguelin in a dose-dependent manner. The disposition of SRC-3 was situated mainly at the nuclear, occasionally in the cytoplasm. Conclusion: Deguelin exhibited potent proliferation inhibition to the human myeloma cell line RPMI-8226 cells, furthermore, deguelin might induce RPMI-8226 cells apoptosis in a dose-dependent manner, which might correspond to the regulation of the expression of SRC-3.
Persistent Identifierhttp://hdl.handle.net/10722/92034
ISSN
2015 SCImago Journal Rankings: 0.130
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Ren_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorShu, W-Xen_HK
dc.date.accessioned2010-09-17T10:34:08Z-
dc.date.available2010-09-17T10:34:08Z-
dc.date.issued2009en_HK
dc.identifier.citationChinese Traditional and Herbal Drugs, 2009, v. 40 n. 7, p. 1086-1090en_HK
dc.identifier.issn0253-2670en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92034-
dc.description.abstractObjective: To investigate the effects of deguelin on the proliferation inhibition and apoptosis induction in RPMI-8226 cells in vitro, as well as the regulation of steroid receptor coactivator-3 (SRC-3) to explore the relationship between them. Methods: The effect of deguelin on the growth of RPMI-8226 cells was studied by MTT assay. Apoptosis was detected through Hoechst 33258 staining and Annexin-V FITC/PI double-labeled cytometry. RT-PCR Technology was applied to assessment of the mRNA expression of SRC-3, whereas, SRC-3 protein expression and localization were determined by using immunohistochemistry method. Results: Deguelin presented striking proliferation inhibition potency on RPMI-8226 cells in vitro and apoptosis induction activity in a time- and dose-dependent manner. The IC50 value for 24 h was (54.55±0.40) nmol/L, (17.04±0.73)% RPMI-8226 cells went apoptotic when treated with 25 nmol/L deguelin for 24 h, with the dose of deguelin increasing to 100 nmol/L, (63.57±1.36)% cells were apoptotic. Over-expression of SRC-3 was found in RPMI-8226 cells, whereas the mRNA and protein expression levels of SRC-3 were significantly downregulated in RPMI-8226 cells induced by deguelin in a dose-dependent manner. The disposition of SRC-3 was situated mainly at the nuclear, occasionally in the cytoplasm. Conclusion: Deguelin exhibited potent proliferation inhibition to the human myeloma cell line RPMI-8226 cells, furthermore, deguelin might induce RPMI-8226 cells apoptosis in a dose-dependent manner, which might correspond to the regulation of the expression of SRC-3.en_HK
dc.languageengen_HK
dc.relation.ispartofChinese Traditional and Herbal Drugsen_HK
dc.subjectApoptosisen_HK
dc.subjectDeguelinen_HK
dc.subjectMultiple Myelomaen_HK
dc.subjectSteroid Receptor Coactivator-3 (Src-3)en_HK
dc.titleEffect on proliferation and apoptosis in RPM1-8226 human myeloma cells and regulation on steroid receptor coactivator-3 by deguelinen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, Y:ychenc@hkucc.hku.hken_HK
dc.identifier.authorityChen, Y=rp1318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-69949128150en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-69949128150&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume40en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1086en_HK
dc.identifier.epage1090en_HK

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