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Article: Study on mismatch repair genes of chronic myeloid leukemia

TitleStudy on mismatch repair genes of chronic myeloid leukemia
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2006
Citation
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi, 2006, v. 27 n. 2, p. 103-106 How to Cite?
AbstractOBJECTIVE: To investigate the expression and regulation mechanism of mismatch repair (MMR) genes in chronic myeloid leukemia (CML). METHODS: Expression of MMR genes hMSH2, hMSH3, hMSH6, hMLH1 and hPMS2 mRNAs in 62 CML patients and K562 cell line were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Expression of bcr-abl mRNA and MMR genes mRNA were detected by RT-PCR in 26 CML patients with allogeneic peripheral blood stem cell transplantation (allo-PBSCT) and 4 CML patients on imatinib treatment. Expression of bcr-abl mRNA was detected by RT-PCR and tyrosine phosphorylation of BCR-ABL fusion protein by Western blot. RESULTS: Expression of hMSH2, hMSH3 and hMLH1 mRNA was significantly lower in CML and K562 cells than in normal control (P < 0.05). In 26 CML with allo-PBSCT and 4 CML patients on imatinib treatment, expressions of hMSH2, hMSH3 and hMLH1 mRNA was enhanced while expression of bcr-abl mRNA decreased. In CML MNC after imatinib treatment and in K562 cells, expression of hMSH2, hMSH3 and hMLH1 mRNA was enhanced while tyrosine phosphorylation of BCR-ABL fusion protein decreased. CONCLUSION: Expressions of hMSH2, hMSH3 and hMLH1 mRNA were down-regulated by bcr-abl fusion gene.
Persistent Identifierhttp://hdl.handle.net/10722/92020
ISSN
2015 SCImago Journal Rankings: 0.137

 

DC FieldValueLanguage
dc.contributor.authorLuo, Jen_HK
dc.contributor.authorPeng, ZGen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorLai, YRen_HK
dc.contributor.authorLu, YYen_HK
dc.contributor.authorSong, SJen_HK
dc.date.accessioned2010-09-17T10:33:42Z-
dc.date.available2010-09-17T10:33:42Z-
dc.date.issued2006en_HK
dc.identifier.citationZhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi, 2006, v. 27 n. 2, p. 103-106en_HK
dc.identifier.issn0253-2727en_HK
dc.identifier.urihttp://hdl.handle.net/10722/92020-
dc.description.abstractOBJECTIVE: To investigate the expression and regulation mechanism of mismatch repair (MMR) genes in chronic myeloid leukemia (CML). METHODS: Expression of MMR genes hMSH2, hMSH3, hMSH6, hMLH1 and hPMS2 mRNAs in 62 CML patients and K562 cell line were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Expression of bcr-abl mRNA and MMR genes mRNA were detected by RT-PCR in 26 CML patients with allogeneic peripheral blood stem cell transplantation (allo-PBSCT) and 4 CML patients on imatinib treatment. Expression of bcr-abl mRNA was detected by RT-PCR and tyrosine phosphorylation of BCR-ABL fusion protein by Western blot. RESULTS: Expression of hMSH2, hMSH3 and hMLH1 mRNA was significantly lower in CML and K562 cells than in normal control (P < 0.05). In 26 CML with allo-PBSCT and 4 CML patients on imatinib treatment, expressions of hMSH2, hMSH3 and hMLH1 mRNA was enhanced while expression of bcr-abl mRNA decreased. In CML MNC after imatinib treatment and in K562 cells, expression of hMSH2, hMSH3 and hMLH1 mRNA was enhanced while tyrosine phosphorylation of BCR-ABL fusion protein decreased. CONCLUSION: Expressions of hMSH2, hMSH3 and hMLH1 mRNA were down-regulated by bcr-abl fusion gene.en_HK
dc.languageengen_HK
dc.relation.ispartofZhonghua xue ye xue za zhi = Zhonghua xueyexue zazhien_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.titleStudy on mismatch repair genes of chronic myeloid leukemiaen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, Y:ychenc@hkucc.hku.hken_HK
dc.identifier.authorityChen, Y=rp1318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid16732964-
dc.identifier.scopuseid_2-s2.0-67651153099en_HK
dc.identifier.volume27en_HK
dc.identifier.issue2en_HK
dc.identifier.spage103en_HK
dc.identifier.epage106en_HK

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