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Article: A procedure for ab initio phasing of one-wavelength anomalous scattering data from proteins - Combining direct methods with the solvent flattening technique

TitleA procedure for ab initio phasing of one-wavelength anomalous scattering data from proteins - Combining direct methods with the solvent flattening technique
Authors
Issue Date1997
Citation
Zeitschrift Fur Kristallographie, 1997, v. 212 n. 2, p. 95-98 How to Cite?
AbstractBoth direct methods and the solvent flattening technique have been proved capable of breaking the phase ambiguity intrinsic in one-wavelength anomalous scattering (OAS) data. However, in theory, while direct methods are better in deriving starting phases from OAS data, the solvent flattening technique is more powerful in the subsequent phase refinement. A procedure is described in this paper which combines direct methods with the solvent flattening technique for phasing of OAS data. The procedure has been tested with the known protein RNase Sa. This protein is of moderate size, and the diffraction data were collected routinely without taking special care for the recording of Friedels. The test proved that the combination of the direct method and the solvent flattening leads to a much better result than that from either method alone.
Persistent Identifierhttp://hdl.handle.net/10722/91954
ISSN
2014 Impact Factor: 1.31
2015 SCImago Journal Rankings: 0.827
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZheng, XFen_HK
dc.contributor.authorGu, YXen_HK
dc.contributor.authorZheng, CDen_HK
dc.contributor.authorMo, YDen_HK
dc.contributor.authorFan, HFen_HK
dc.contributor.authorHao, Qen_HK
dc.date.accessioned2010-09-17T10:31:34Z-
dc.date.available2010-09-17T10:31:34Z-
dc.date.issued1997en_HK
dc.identifier.citationZeitschrift Fur Kristallographie, 1997, v. 212 n. 2, p. 95-98en_HK
dc.identifier.issn0044-2968en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91954-
dc.description.abstractBoth direct methods and the solvent flattening technique have been proved capable of breaking the phase ambiguity intrinsic in one-wavelength anomalous scattering (OAS) data. However, in theory, while direct methods are better in deriving starting phases from OAS data, the solvent flattening technique is more powerful in the subsequent phase refinement. A procedure is described in this paper which combines direct methods with the solvent flattening technique for phasing of OAS data. The procedure has been tested with the known protein RNase Sa. This protein is of moderate size, and the diffraction data were collected routinely without taking special care for the recording of Friedels. The test proved that the combination of the direct method and the solvent flattening leads to a much better result than that from either method alone.en_HK
dc.languageengen_HK
dc.relation.ispartofZeitschrift fur Kristallographieen_HK
dc.titleA procedure for ab initio phasing of one-wavelength anomalous scattering data from proteins - Combining direct methods with the solvent flattening techniqueen_HK
dc.typeArticleen_HK
dc.identifier.emailHao, Q: qhao@hku.hken_HK
dc.identifier.authorityHao, Q=rp01332en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1524/zkri.1997.212.2.95en_US
dc.identifier.scopuseid_2-s2.0-1542711540en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1542711540&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume212en_HK
dc.identifier.issue2en_HK
dc.identifier.spage95en_HK
dc.identifier.epage98en_HK
dc.identifier.isiWOS:A1997WJ40000002-
dc.identifier.scopusauthoridZheng, XF=36766092600en_HK
dc.identifier.scopusauthoridGu, YX=7403046284en_HK
dc.identifier.scopusauthoridZheng, CD=7401934929en_HK
dc.identifier.scopusauthoridMo, YD=7202961906en_HK
dc.identifier.scopusauthoridFan, HF=7402554116en_HK
dc.identifier.scopusauthoridHao, Q=7102508868en_HK

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