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Article: S-SWAT (softer single-wavelength anomalous technique): Potential in high-throughput protein crystallography

TitleS-SWAT (softer single-wavelength anomalous technique): Potential in high-throughput protein crystallography
Authors
Issue Date2003
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0108-7673&site=1
Citation
Acta Crystallographica Section A: Foundations Of Crystallography, 2003, v. 59 n. 4, p. 327-334 How to Cite?
AbstractThe drive for yet higher output of protein crystal structures sustains an interest in streamlining data-collection protocols. Highly organized automated robotics attend to one aspect. The data-collection elapsed time also depends on the number of wavelengths used, the data redundancy and the desired diffraction resolution limit. Synchrotron-radiation beamlines offer a centralized data-collection approach with considerable flexibility of choice of wavelength(s) with high beam intensity and fine collimation. The arrival of automated IPs (imaging plates) and CCDs (charge-coupled devices) with sample freezing has widened the scope for ease of data collection and achieving a high redundancy, the latter at the cost of elapsed time per sample. This paper reports a single-wavelength approach where a large optimized f″ signal involving the Xe L1-absorption edge is harnessed. Even with modest data redundancy (7), a high-quality electron-density map was obtained from that single data set when combined with phase improvement methods. Two phasing protocols are compared for the same data set. Resolution extension (to 1.4 Å), in this test, was also done but via a second data set. In future, with a tilted detector geometry, full diffraction resolution will be collectable in one experiment on the one beamline. Moreover, careful minimization of beamline Be window thickness on the new Daresbury SRS Multipole Wiggler Beamline 10 will maximize the 2-3 Å (softer) wavelength intensity performance specification. The work has application to the harnessing of iodine f″ optimized signals and enhanced sulfur f″, as well as xenon f″, in protein crystallography. This approach, softer-SWAT, should 'swat' many protein structures when it comes online. © 2003 International Union of Crystallography Printed in Great Britain - all rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/91946
ISSN
2014 Impact Factor: 2.325
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorOlczak, Aen_HK
dc.contributor.authorCianci, Men_HK
dc.contributor.authorHao, Qen_HK
dc.contributor.authorRizkallah, PJen_HK
dc.contributor.authorRaftery, Jen_HK
dc.contributor.authorHelliwell, JRen_HK
dc.date.accessioned2010-09-17T10:31:20Z-
dc.date.available2010-09-17T10:31:20Z-
dc.date.issued2003en_HK
dc.identifier.citationActa Crystallographica Section A: Foundations Of Crystallography, 2003, v. 59 n. 4, p. 327-334en_HK
dc.identifier.issn0108-7673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91946-
dc.description.abstractThe drive for yet higher output of protein crystal structures sustains an interest in streamlining data-collection protocols. Highly organized automated robotics attend to one aspect. The data-collection elapsed time also depends on the number of wavelengths used, the data redundancy and the desired diffraction resolution limit. Synchrotron-radiation beamlines offer a centralized data-collection approach with considerable flexibility of choice of wavelength(s) with high beam intensity and fine collimation. The arrival of automated IPs (imaging plates) and CCDs (charge-coupled devices) with sample freezing has widened the scope for ease of data collection and achieving a high redundancy, the latter at the cost of elapsed time per sample. This paper reports a single-wavelength approach where a large optimized f″ signal involving the Xe L1-absorption edge is harnessed. Even with modest data redundancy (7), a high-quality electron-density map was obtained from that single data set when combined with phase improvement methods. Two phasing protocols are compared for the same data set. Resolution extension (to 1.4 Å), in this test, was also done but via a second data set. In future, with a tilted detector geometry, full diffraction resolution will be collectable in one experiment on the one beamline. Moreover, careful minimization of beamline Be window thickness on the new Daresbury SRS Multipole Wiggler Beamline 10 will maximize the 2-3 Å (softer) wavelength intensity performance specification. The work has application to the harnessing of iodine f″ optimized signals and enhanced sulfur f″, as well as xenon f″, in protein crystallography. This approach, softer-SWAT, should 'swat' many protein structures when it comes online. © 2003 International Union of Crystallography Printed in Great Britain - all rights reserved.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0108-7673&site=1en_HK
dc.relation.ispartofActa Crystallographica Section A: Foundations of Crystallographyen_HK
dc.titleS-SWAT (softer single-wavelength anomalous technique): Potential in high-throughput protein crystallographyen_HK
dc.typeArticleen_HK
dc.identifier.emailHao, Q: qhao@hku.hken_HK
dc.identifier.authorityHao, Q=rp01332en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1107/S0108767303009693en_HK
dc.identifier.pmid12832811-
dc.identifier.scopuseid_2-s2.0-0038152788en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038152788&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume59en_HK
dc.identifier.issue4en_HK
dc.identifier.spage327en_HK
dc.identifier.epage334en_HK
dc.identifier.isiWOS:000184204100005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridOlczak, A=6701829259en_HK
dc.identifier.scopusauthoridCianci, M=35730836300en_HK
dc.identifier.scopusauthoridHao, Q=7102508868en_HK
dc.identifier.scopusauthoridRizkallah, PJ=6603681152en_HK
dc.identifier.scopusauthoridRaftery, J=7004558116en_HK
dc.identifier.scopusauthoridHelliwell, JR=8693358800en_HK

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