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Article: Is single-wavelength anomalous scattering sufficient for solving phases? A comparison of different methods for a 2.1 Å structure solution

TitleIs single-wavelength anomalous scattering sufficient for solving phases? A comparison of different methods for a 2.1 Å structure solution
Authors
Issue Date1999
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/editors.asp?ref=0907-4449&site=1
Citation
Acta Crystallographica Section D: Biological Crystallography, 1999, v. 55 n. 9, p. 1620-1622 How to Cite?
AbstractThe structure of rusticyanin is the largest unknown structure (M(r) = 16.8 kDa) which has been recently solved by the direct-methods approach using only single-wavelength anomalous scattering (SAS) data from the native protein [Harvey et. al. (1998). Acta Cryst. D54, 629-635]. Here, the results of the Sim distribution approach [Hendrikson and Teeter (1981). Nature (London), 290, 107-113] and of the CCP4 procedure MLPHARE [Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760-763] are compared with those from direct methods. Analysis against the final refined model shows that direct methods produced significantly better electron-density maps than the Sim distribution and MLPHARE approaches (average phase error was around 63°in both cases).
Persistent Identifierhttp://hdl.handle.net/10722/91942
ISSN
2013 Impact Factor: 7.232
2015 SCImago Journal Rankings: 3.088
References

 

DC FieldValueLanguage
dc.contributor.authorYuDong, Len_HK
dc.contributor.authorHarvey, Ien_HK
dc.contributor.authorYuanXin, Gen_HK
dc.contributor.authorChaoDe, Zen_HK
dc.contributor.authorYiZong, Hen_HK
dc.contributor.authorHaiFu, Fen_HK
dc.contributor.authorHasnain, SSen_HK
dc.contributor.authorHao, Qen_HK
dc.date.accessioned2010-09-17T10:31:13Z-
dc.date.available2010-09-17T10:31:13Z-
dc.date.issued1999en_HK
dc.identifier.citationActa Crystallographica Section D: Biological Crystallography, 1999, v. 55 n. 9, p. 1620-1622en_HK
dc.identifier.issn0907-4449en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91942-
dc.description.abstractThe structure of rusticyanin is the largest unknown structure (M(r) = 16.8 kDa) which has been recently solved by the direct-methods approach using only single-wavelength anomalous scattering (SAS) data from the native protein [Harvey et. al. (1998). Acta Cryst. D54, 629-635]. Here, the results of the Sim distribution approach [Hendrikson and Teeter (1981). Nature (London), 290, 107-113] and of the CCP4 procedure MLPHARE [Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760-763] are compared with those from direct methods. Analysis against the final refined model shows that direct methods produced significantly better electron-density maps than the Sim distribution and MLPHARE approaches (average phase error was around 63°in both cases).en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/editors.asp?ref=0907-4449&site=1en_HK
dc.relation.ispartofActa Crystallographica Section D: Biological Crystallographyen_HK
dc.titleIs single-wavelength anomalous scattering sufficient for solving phases? A comparison of different methods for a 2.1 Å structure solutionen_HK
dc.typeArticleen_HK
dc.identifier.emailHao, Q: qhao@hku.hken_HK
dc.identifier.authorityHao, Q=rp01332en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1107/S0907444999007726en_HK
dc.identifier.pmid10489467-
dc.identifier.scopuseid_2-s2.0-13044283441en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-13044283441&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume55en_HK
dc.identifier.issue9en_HK
dc.identifier.spage1620en_HK
dc.identifier.epage1622en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYuDong, L=6504138838en_HK
dc.identifier.scopusauthoridHarvey, I=35944665600en_HK
dc.identifier.scopusauthoridYuanXin, G=6505703892en_HK
dc.identifier.scopusauthoridChaoDe, Z=6507990395en_HK
dc.identifier.scopusauthoridYiZong, H=6504705160en_HK
dc.identifier.scopusauthoridHaiFu, F=6505990871en_HK
dc.identifier.scopusauthoridHasnain, SS=7102767936en_HK
dc.identifier.scopusauthoridHao, Q=7102508868en_HK

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