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Article: Structure solution of azurin II from Alcaligenes xylosoxidans using the Laue method: Possibility of studying in situ redox changes using X-rays

TitleStructure solution of azurin II from Alcaligenes xylosoxidans using the Laue method: Possibility of studying in situ redox changes using X-rays
Authors
KeywordsAzurin
Data completeness
Denitrification
Electron transfer
Laue method
Redox reaction
Time-resolved studies
Issue Date1996
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JSY
Citation
Journal Of Synchrotron Radiation, 1996, v. 3 n. 1, p. 14-19 How to Cite?
AbstractWe have recently demonstrated that X-rays can be used for changing the redox states of the metal centre in metalloproteins [Murphy et al. (1995). J. Synchrotron Rad. 2, 64-69]. The possibility of using the Laue method for studying the structural changes associated with such X-ray-induced reactions is explored by applying the method to the structure determination of a new azurin (hereafter referred to as azurin II) from the denitrifying bacterium Alcaligenes xylosoxidans. Laue X-ray diffraction data of azurin II were collected at station 9.7 of the SRS Daresbury. Three diffraction patterns were recorded on film packs at three different crystal orientations. The data were processed using the Daresbury Laue Software Suite to give 2224 independent single reflections (Rmerge = 0.136) in the wavelength range 0.36-1.40 Å. The data completeness was 44% at 2.55 Å resolution. Phase determination for the data was undertaken using the molecular-replacement method; the top peak was chosen in both the rotation function and the subsequent translation function. This solution agreed well with the molecular-replacement solution achieved independently using monochromatic data. The electron-density map showed reasonably good agreement with the model and the copper site was readily recognizable as it had the highest density. To see if the electron-density map could be improved, the doublets in the diffraction data were then deconvoluted. This added 26% data in the region ∞-2dmin resulting in an improvement in the data completeness to 50% and thus in improved continuity of the electron-density map. The quality of these maps is discussed from the point of view of the suitability of this approach for studying redox-induced structural changes.
Persistent Identifierhttp://hdl.handle.net/10722/91930
ISSN
2014 Impact Factor: 2.794
2015 SCImago Journal Rankings: 1.211
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDebenham, MJen_HK
dc.contributor.authorHao, Qen_HK
dc.contributor.authorHasnain, SSen_HK
dc.contributor.authorDodd, FEen_HK
dc.contributor.authorAbraham, ZHLen_HK
dc.contributor.authorEady, RRen_HK
dc.date.accessioned2010-09-17T10:30:51Z-
dc.date.available2010-09-17T10:30:51Z-
dc.date.issued1996en_HK
dc.identifier.citationJournal Of Synchrotron Radiation, 1996, v. 3 n. 1, p. 14-19en_HK
dc.identifier.issn0909-0495en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91930-
dc.description.abstractWe have recently demonstrated that X-rays can be used for changing the redox states of the metal centre in metalloproteins [Murphy et al. (1995). J. Synchrotron Rad. 2, 64-69]. The possibility of using the Laue method for studying the structural changes associated with such X-ray-induced reactions is explored by applying the method to the structure determination of a new azurin (hereafter referred to as azurin II) from the denitrifying bacterium Alcaligenes xylosoxidans. Laue X-ray diffraction data of azurin II were collected at station 9.7 of the SRS Daresbury. Three diffraction patterns were recorded on film packs at three different crystal orientations. The data were processed using the Daresbury Laue Software Suite to give 2224 independent single reflections (Rmerge = 0.136) in the wavelength range 0.36-1.40 Å. The data completeness was 44% at 2.55 Å resolution. Phase determination for the data was undertaken using the molecular-replacement method; the top peak was chosen in both the rotation function and the subsequent translation function. This solution agreed well with the molecular-replacement solution achieved independently using monochromatic data. The electron-density map showed reasonably good agreement with the model and the copper site was readily recognizable as it had the highest density. To see if the electron-density map could be improved, the doublets in the diffraction data were then deconvoluted. This added 26% data in the region ∞-2dmin resulting in an improvement in the data completeness to 50% and thus in improved continuity of the electron-density map. The quality of these maps is discussed from the point of view of the suitability of this approach for studying redox-induced structural changes.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JSYen_HK
dc.relation.ispartofJournal of Synchrotron Radiationen_HK
dc.subjectAzurinen_HK
dc.subjectData completenessen_HK
dc.subjectDenitrificationen_HK
dc.subjectElectron transferen_HK
dc.subjectLaue methoden_HK
dc.subjectRedox reactionen_HK
dc.subjectTime-resolved studiesen_HK
dc.titleStructure solution of azurin II from Alcaligenes xylosoxidans using the Laue method: Possibility of studying in situ redox changes using X-raysen_HK
dc.typeArticleen_HK
dc.identifier.emailHao, Q: qhao@hku.hken_HK
dc.identifier.authorityHao, Q=rp01332en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1107/s0909049595013240en_US
dc.identifier.scopuseid_2-s2.0-0030490696en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030490696&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume3en_HK
dc.identifier.issue1en_HK
dc.identifier.spage14en_HK
dc.identifier.epage19en_HK
dc.identifier.isiWOS:A1996TX75500003-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridDebenham, MJ=6603249598en_HK
dc.identifier.scopusauthoridHao, Q=7102508868en_HK
dc.identifier.scopusauthoridHasnain, SS=7102767936en_HK
dc.identifier.scopusauthoridDodd, FE=7004001288en_HK
dc.identifier.scopusauthoridAbraham, ZHL=7006098225en_HK
dc.identifier.scopusauthoridEady, RR=7103375066en_HK

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