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Article: Direct-method-aided phasing of MAD data

TitleDirect-method-aided phasing of MAD data
Authors
Issue Date2001
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/editors.asp?ref=0907-4449&site=1
Citation
Acta Crystallographica Section D: Biological Crystallography, 2001, v. 57 n. 2, p. 250-253 How to Cite?
AbstractThe direct methods of breaking the phase ambiguity intrinsic in one-wavelength anomalous scattering (OAS) data and MAD phasing are powerful methods in their own rights. In a different context, in addition to their success in phasing OAS data, direct methods can also be useful in the treatment of MAD data. The idea has been tested with the MAD data at 2.5 Å resolution from the protein human adenosine kinase [Mathews et al. (1998), Biochemistry, 37, 15607-15620]. The results showed that the incorporation of direct methods in MAD phasing led to a significant improvement of phases over those obtained from the conventional MAD phasing method alone, as indicated by improved map correlation coefficients (with the existing model), reduced phase errors by 4.5° and improved map connectivity.
Persistent Identifierhttp://hdl.handle.net/10722/91929
ISSN
2013 Impact Factor: 7.232
2015 SCImago Journal Rankings: 3.088
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGu, YXen_HK
dc.contributor.authorLiu, YDen_HK
dc.contributor.authorHao, Qen_HK
dc.contributor.authorEalick, SEen_HK
dc.contributor.authorFan, HFen_HK
dc.date.accessioned2010-09-17T10:30:50Z-
dc.date.available2010-09-17T10:30:50Z-
dc.date.issued2001en_HK
dc.identifier.citationActa Crystallographica Section D: Biological Crystallography, 2001, v. 57 n. 2, p. 250-253en_HK
dc.identifier.issn0907-4449en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91929-
dc.description.abstractThe direct methods of breaking the phase ambiguity intrinsic in one-wavelength anomalous scattering (OAS) data and MAD phasing are powerful methods in their own rights. In a different context, in addition to their success in phasing OAS data, direct methods can also be useful in the treatment of MAD data. The idea has been tested with the MAD data at 2.5 Å resolution from the protein human adenosine kinase [Mathews et al. (1998), Biochemistry, 37, 15607-15620]. The results showed that the incorporation of direct methods in MAD phasing led to a significant improvement of phases over those obtained from the conventional MAD phasing method alone, as indicated by improved map correlation coefficients (with the existing model), reduced phase errors by 4.5° and improved map connectivity.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/editors.asp?ref=0907-4449&site=1en_HK
dc.relation.ispartofActa Crystallographica Section D: Biological Crystallographyen_HK
dc.titleDirect-method-aided phasing of MAD dataen_HK
dc.typeArticleen_HK
dc.identifier.emailHao, Q: qhao@hku.hken_HK
dc.identifier.authorityHao, Q=rp01332en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1107/S0907444900018606en_HK
dc.identifier.pmid11173471-
dc.identifier.scopuseid_2-s2.0-0035125233en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035125233&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume57en_HK
dc.identifier.issue2en_HK
dc.identifier.spage250en_HK
dc.identifier.epage253en_HK
dc.identifier.isiWOS:000166598300010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGu, YX=7403046284en_HK
dc.identifier.scopusauthoridLiu, YD=12803269300en_HK
dc.identifier.scopusauthoridHao, Q=7102508868en_HK
dc.identifier.scopusauthoridEalick, SE=7006610617en_HK
dc.identifier.scopusauthoridFan, HF=7402554116en_HK

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