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Article: Direct phasing of one-wavelength anomalous-scattering data

TitleDirect phasing of one-wavelength anomalous-scattering data
Authors
KeywordsDirect methods
One-wavelength anomalous scattering
Issue Date2000
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JSY
Citation
Journal Of Synchrotron Radiation, 2000, v. 7 n. 3, p. 148-151 How to Cite?
AbstractThis paper presents a brief survey of methods in ab initio phasing of one-wavelength anomalous-scattering data. In particular, the method implemented in the computer program OASIS has been tested using two new data sets from orotidine 5′-monophosphate decarboxylase (OMPDC) [Appleby et al. (2000). Proc. Natl Acad. Sci. USA. In the press] and PurE [Mathews et al. (1999). Structure, 7(11), 1395-1406]. The Se atoms were located by the small-molecule program SAPI. The electron density maps after OASIS and density modification for both structures clearly revealed the Cα trace and, in the case of PurE, most side-chains. The test with the OMPDC data demonstrated that, by exploiting the anomalous signal at a single wavelength, direct methods can be used to determine phases at moderate (∼2.5 Å) macromolecular crystallographic resolution for a large-size protein (5663 non-H atoms in the asymmetric unit). The exceptionally good quality of the electron map shown in the case of PurE suggested that fully automatic model fitting is possible.
Persistent Identifierhttp://hdl.handle.net/10722/91922
ISSN
2014 Impact Factor: 2.794
2015 SCImago Journal Rankings: 1.211
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHao, Qen_HK
dc.date.accessioned2010-09-17T10:30:37Z-
dc.date.available2010-09-17T10:30:37Z-
dc.date.issued2000en_HK
dc.identifier.citationJournal Of Synchrotron Radiation, 2000, v. 7 n. 3, p. 148-151en_HK
dc.identifier.issn0909-0495en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91922-
dc.description.abstractThis paper presents a brief survey of methods in ab initio phasing of one-wavelength anomalous-scattering data. In particular, the method implemented in the computer program OASIS has been tested using two new data sets from orotidine 5′-monophosphate decarboxylase (OMPDC) [Appleby et al. (2000). Proc. Natl Acad. Sci. USA. In the press] and PurE [Mathews et al. (1999). Structure, 7(11), 1395-1406]. The Se atoms were located by the small-molecule program SAPI. The electron density maps after OASIS and density modification for both structures clearly revealed the Cα trace and, in the case of PurE, most side-chains. The test with the OMPDC data demonstrated that, by exploiting the anomalous signal at a single wavelength, direct methods can be used to determine phases at moderate (∼2.5 Å) macromolecular crystallographic resolution for a large-size protein (5663 non-H atoms in the asymmetric unit). The exceptionally good quality of the electron map shown in the case of PurE suggested that fully automatic model fitting is possible.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JSYen_HK
dc.relation.ispartofJournal of Synchrotron Radiationen_HK
dc.subjectDirect methodsen_HK
dc.subjectOne-wavelength anomalous scatteringen_HK
dc.titleDirect phasing of one-wavelength anomalous-scattering dataen_HK
dc.typeArticleen_HK
dc.identifier.emailHao, Q: qhao@hku.hken_HK
dc.identifier.authorityHao, Q=rp01332en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1107/s0909049500003010en_US
dc.identifier.scopuseid_2-s2.0-0034343956en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034343956&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue3en_HK
dc.identifier.spage148en_HK
dc.identifier.epage151en_HK
dc.identifier.isiWOS:000087250300007-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridHao, Q=7102508868en_HK

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