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Article: High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths

TitleHigh-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths
Authors
KeywordsCapillary cryoprotection
High-pressure cryocooling
SAD phasing
Sulfur
Xenon
Issue Date2007
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/editors.asp?ref=0907-4449&site=1
Citation
Acta Crystallographica Section D: Biological Crystallography, 2007, v. 63 n. 5, p. 653-659 How to Cite?
AbstractCrystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography. © International Union of Crystallography 2007.
Persistent Identifierhttp://hdl.handle.net/10722/91891
ISSN
2013 Impact Factor: 7.232
2015 SCImago Journal Rankings: 3.088
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKim, CUen_HK
dc.contributor.authorHao, Qen_HK
dc.contributor.authorGruner, SMen_HK
dc.date.accessioned2010-09-17T10:29:41Z-
dc.date.available2010-09-17T10:29:41Z-
dc.date.issued2007en_HK
dc.identifier.citationActa Crystallographica Section D: Biological Crystallography, 2007, v. 63 n. 5, p. 653-659en_HK
dc.identifier.issn0907-4449en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91891-
dc.description.abstractCrystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography. © International Union of Crystallography 2007.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/editors.asp?ref=0907-4449&site=1en_HK
dc.relation.ispartofActa Crystallographica Section D: Biological Crystallographyen_HK
dc.subjectCapillary cryoprotectionen_HK
dc.subjectHigh-pressure cryocoolingen_HK
dc.subjectSAD phasingen_HK
dc.subjectSulfuren_HK
dc.subjectXenonen_HK
dc.titleHigh-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengthsen_HK
dc.typeArticleen_HK
dc.identifier.emailHao, Q: qhao@hku.hken_HK
dc.identifier.authorityHao, Q=rp01332en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1107/S0907444907011924en_HK
dc.identifier.pmid17452791-
dc.identifier.scopuseid_2-s2.0-34247524718en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34247524718&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume63en_HK
dc.identifier.issue5en_HK
dc.identifier.spage653en_HK
dc.identifier.epage659en_HK
dc.identifier.isiWOS:000248009000012-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKim, CU=8730900600en_HK
dc.identifier.scopusauthoridHao, Q=7102508868en_HK
dc.identifier.scopusauthoridGruner, SM=7102958350en_HK
dc.identifier.citeulike1200608-

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