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Article: Combining direct methods with isomorphous replacement or anomalous scattering data. VIII. Phasing experimental SIR data with the replacing atoms in a centrosymmetric arrangement

TitleCombining direct methods with isomorphous replacement or anomalous scattering data. VIII. Phasing experimental SIR data with the replacing atoms in a centrosymmetric arrangement
Authors
Issue Date1999
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/editors.asp?ref=0907-4449&site=1
Citation
Acta Crystallographica Section D: Biological Crystallography, 1999, v. 55 n. 4, p. 846-848 How to Cite?
AbstractA multisolution direct method has been proposed to resolve R the phase ambiguity intrinsic in single isomorphous replacement data of proteins with the replacing atoms in a centrosymmetric arrangement. The phase ambiguity of each reflection is in fact a 'sign ambiguity' of the phase difference between the phase of the native protein and that of the replacing atoms, i.e. ± |Δφ| = φ - φ'. The P+ probability formula can be used to derive the signs. The multisolution phasing procedure is initiated using random starting values of P+. A cluster analysis is used instead of figures of merit to find the correct solution. The direct-method phases can be further improved by density-modification techniques. The method was tested with the experimental SIR data at 2 Å resolution from a known protein aPP; satisfactory results were obtained.
Persistent Identifierhttp://hdl.handle.net/10722/91887
ISSN
2013 Impact Factor: 7.232
2015 SCImago Journal Rankings: 3.088
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, YDen_HK
dc.contributor.authorGu, YXen_HK
dc.contributor.authorZheng, CDen_HK
dc.contributor.authorHao, Qen_HK
dc.contributor.authorFan, HFen_HK
dc.date.accessioned2010-09-17T10:29:34Z-
dc.date.available2010-09-17T10:29:34Z-
dc.date.issued1999en_HK
dc.identifier.citationActa Crystallographica Section D: Biological Crystallography, 1999, v. 55 n. 4, p. 846-848en_HK
dc.identifier.issn0907-4449en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91887-
dc.description.abstractA multisolution direct method has been proposed to resolve R the phase ambiguity intrinsic in single isomorphous replacement data of proteins with the replacing atoms in a centrosymmetric arrangement. The phase ambiguity of each reflection is in fact a 'sign ambiguity' of the phase difference between the phase of the native protein and that of the replacing atoms, i.e. ± |Δφ| = φ - φ'. The P+ probability formula can be used to derive the signs. The multisolution phasing procedure is initiated using random starting values of P+. A cluster analysis is used instead of figures of merit to find the correct solution. The direct-method phases can be further improved by density-modification techniques. The method was tested with the experimental SIR data at 2 Å resolution from a known protein aPP; satisfactory results were obtained.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/editors.asp?ref=0907-4449&site=1en_HK
dc.relation.ispartofActa Crystallographica Section D: Biological Crystallographyen_HK
dc.titleCombining direct methods with isomorphous replacement or anomalous scattering data. VIII. Phasing experimental SIR data with the replacing atoms in a centrosymmetric arrangementen_HK
dc.typeArticleen_HK
dc.identifier.emailHao, Q: qhao@hku.hken_HK
dc.identifier.authorityHao, Q=rp01332en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1107/S0907444998017703en_HK
dc.identifier.pmid10089315-
dc.identifier.scopuseid_2-s2.0-0033119225en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033119225&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume55en_HK
dc.identifier.issue4en_HK
dc.identifier.spage846en_HK
dc.identifier.epage848en_HK
dc.identifier.isiWOS:000079855200016-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLiu, YD=12803269300en_HK
dc.identifier.scopusauthoridGu, YX=7403046284en_HK
dc.identifier.scopusauthoridZheng, CD=7401934929en_HK
dc.identifier.scopusauthoridHao, Q=7102508868en_HK
dc.identifier.scopusauthoridFan, HF=7402554116en_HK

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