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Article: Direct-method structure determination of the native Azurin II protein using one-wavelength anomalous scattering data

TitleDirect-method structure determination of the native Azurin II protein using one-wavelength anomalous scattering data
Authors
Issue Date1996
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/editors.asp?ref=0907-4449&site=1
Citation
Acta Crystallographica Section D: Biological Crystallography, 1996, v. 52 n. 5, p. 937-941 How to Cite?
AbstractThe one-wavelength anomalous scattering (OAS) X-ray diffraction data of azurin II, a copper-containing protein from Alcaligenes xylosoxidans were collected at the Photon Factory, Japan at a 'routine' wavelength of 0.97 Å. The structure had been originally solved by the molecular-replacement method [Dodd, Hasnain, Abraham, Eady and Smith (1995). Acta Cryst. D51, 1052-1064]. As a technique of ab initio structure determination, the direct method [Fan, Hao, Gu, Qian, Zheng and Ke (1990). Acta Cryst. A46, 935-939] was attempted to break the phase ambiguity intrinsic to OAS data. The phases were then improved using the solvent-flattening method. The final electron-density map clearly shows most Cα positions and many side chains and it is traceable without prior knowledge of the structure. It is concluded that the direct method is capable of phasing anomalous scattering data collected at one wavelength from moderate-sized native proteins (M(w) ≃ 20 kDa) which contain copper or atoms with a similar scattering power.
Persistent Identifierhttp://hdl.handle.net/10722/91884
ISSN
2013 Impact Factor: 7.232
2015 SCImago Journal Rankings: 3.088
References

 

DC FieldValueLanguage
dc.contributor.authorZheng, XFen_HK
dc.contributor.authorFan, HFen_HK
dc.contributor.authorHao, Qen_HK
dc.contributor.authorDodd, FEen_HK
dc.contributor.authorHasnain, SSen_HK
dc.date.accessioned2010-09-17T10:29:28Z-
dc.date.available2010-09-17T10:29:28Z-
dc.date.issued1996en_HK
dc.identifier.citationActa Crystallographica Section D: Biological Crystallography, 1996, v. 52 n. 5, p. 937-941en_HK
dc.identifier.issn0907-4449en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91884-
dc.description.abstractThe one-wavelength anomalous scattering (OAS) X-ray diffraction data of azurin II, a copper-containing protein from Alcaligenes xylosoxidans were collected at the Photon Factory, Japan at a 'routine' wavelength of 0.97 Å. The structure had been originally solved by the molecular-replacement method [Dodd, Hasnain, Abraham, Eady and Smith (1995). Acta Cryst. D51, 1052-1064]. As a technique of ab initio structure determination, the direct method [Fan, Hao, Gu, Qian, Zheng and Ke (1990). Acta Cryst. A46, 935-939] was attempted to break the phase ambiguity intrinsic to OAS data. The phases were then improved using the solvent-flattening method. The final electron-density map clearly shows most Cα positions and many side chains and it is traceable without prior knowledge of the structure. It is concluded that the direct method is capable of phasing anomalous scattering data collected at one wavelength from moderate-sized native proteins (M(w) ≃ 20 kDa) which contain copper or atoms with a similar scattering power.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/editors.asp?ref=0907-4449&site=1en_HK
dc.relation.ispartofActa Crystallographica Section D: Biological Crystallographyen_HK
dc.titleDirect-method structure determination of the native Azurin II protein using one-wavelength anomalous scattering dataen_HK
dc.typeArticleen_HK
dc.identifier.emailHao, Q: qhao@hku.hken_HK
dc.identifier.authorityHao, Q=rp01332en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1107/S0907444996003939en_HK
dc.identifier.scopuseid_2-s2.0-0030484257en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030484257&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume52en_HK
dc.identifier.issue5en_HK
dc.identifier.spage937en_HK
dc.identifier.epage941en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZheng, XF=36766092600en_HK
dc.identifier.scopusauthoridFan, HF=7402554116en_HK
dc.identifier.scopusauthoridHao, Q=7102508868en_HK
dc.identifier.scopusauthoridDodd, FE=7004001288en_HK
dc.identifier.scopusauthoridHasnain, SS=7102767936en_HK

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