File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Phosphorylcholine-polycation diblock copolymers as synthetic vectors for gene delivery

TitlePhosphorylcholine-polycation diblock copolymers as synthetic vectors for gene delivery
Authors
KeywordsNon-viral gene delivery
Polyelectrolyte complexes
Steric stabilization
Transfection
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jconrel
Citation
Journal Of Controlled Release, 2004, v. 100 n. 2, p. 293-312 How to Cite?
AbstractA novel 2-(dimethylamino)ethyl methacrylate-block-2-(methacryloyloxyethyl phosphorylcholine) (DMAEMA-MPC) diblock copolymer was synthesized and investigated as a new non-viral vector for gene delivery. The attractive perspective of this phosphorylcholine (PC)-based material is its propensity to condense DNA efficiently via the cationic DMAEMA block, as previously demonstrated for the respective homopolymer, with the MPC block acting as a biocompatible steric stabilizer. Two series of DMAEMA-MPC diblock copolymers were synthesized for evaluation, varying independently and systematically either MPC or DMAEMA block length. Markedly different DNA-copolymer complexes were observed depending on the copolymer molecular composition. Certain polymeric structures led to formation of highly condensed, sterically stabilized DNA complexes of 120-140 nm diameter, while some resulted in partly condensed DNA-polymer complexes with 'spaghetti' structures, indicating the importance of a copolymer composition to balance condensing and steric stabilization effect. A low level of non-specific cellular association of the complexes with optimized physicochemical properties was seen, indicating the role of MPC surface layer in the interactions with biological membranes and important property in preventing promiscuous interactions with tissues in the body and potentially allowing for cellular specific delivery of the condensates following the attachment of a targeting ligand. © 2004 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/91761
ISSN
2015 Impact Factor: 7.441
2015 SCImago Journal Rankings: 2.827
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLam, JKWen_HK
dc.contributor.authorMa, Yen_HK
dc.contributor.authorArmes, SPen_HK
dc.contributor.authorLewis, ALen_HK
dc.contributor.authorBaldwin, Ten_HK
dc.contributor.authorStolnik, Sen_HK
dc.date.accessioned2010-09-17T10:25:47Z-
dc.date.available2010-09-17T10:25:47Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Controlled Release, 2004, v. 100 n. 2, p. 293-312en_HK
dc.identifier.issn0168-3659en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91761-
dc.description.abstractA novel 2-(dimethylamino)ethyl methacrylate-block-2-(methacryloyloxyethyl phosphorylcholine) (DMAEMA-MPC) diblock copolymer was synthesized and investigated as a new non-viral vector for gene delivery. The attractive perspective of this phosphorylcholine (PC)-based material is its propensity to condense DNA efficiently via the cationic DMAEMA block, as previously demonstrated for the respective homopolymer, with the MPC block acting as a biocompatible steric stabilizer. Two series of DMAEMA-MPC diblock copolymers were synthesized for evaluation, varying independently and systematically either MPC or DMAEMA block length. Markedly different DNA-copolymer complexes were observed depending on the copolymer molecular composition. Certain polymeric structures led to formation of highly condensed, sterically stabilized DNA complexes of 120-140 nm diameter, while some resulted in partly condensed DNA-polymer complexes with 'spaghetti' structures, indicating the importance of a copolymer composition to balance condensing and steric stabilization effect. A low level of non-specific cellular association of the complexes with optimized physicochemical properties was seen, indicating the role of MPC surface layer in the interactions with biological membranes and important property in preventing promiscuous interactions with tissues in the body and potentially allowing for cellular specific delivery of the condensates following the attachment of a targeting ligand. © 2004 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jconrelen_HK
dc.relation.ispartofJournal of Controlled Releaseen_HK
dc.subjectNon-viral gene deliveryen_HK
dc.subjectPolyelectrolyte complexesen_HK
dc.subjectSteric stabilizationen_HK
dc.subjectTransfectionen_HK
dc.titlePhosphorylcholine-polycation diblock copolymers as synthetic vectors for gene deliveryen_HK
dc.typeArticleen_HK
dc.identifier.emailLam, JKW: jkwlam@hku.hken_HK
dc.identifier.authorityLam, JKW=rp01346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jconrel.2004.08.028en_HK
dc.identifier.pmid15544876en_HK
dc.identifier.scopuseid_2-s2.0-8544261100en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-8544261100&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume100en_HK
dc.identifier.issue2en_HK
dc.identifier.spage293en_HK
dc.identifier.epage312en_HK
dc.identifier.isiWOS:000225847700013-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLam, JKW=8404243000en_HK
dc.identifier.scopusauthoridMa, Y=35187665400en_HK
dc.identifier.scopusauthoridArmes, SP=7103125898en_HK
dc.identifier.scopusauthoridLewis, AL=35305215700en_HK
dc.identifier.scopusauthoridBaldwin, T=55245007600en_HK
dc.identifier.scopusauthoridStolnik, S=35515143300en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats