File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Folate conjugated phosphorylcholine-based polycations for specific targeting in nucleic acids delivery

TitleFolate conjugated phosphorylcholine-based polycations for specific targeting in nucleic acids delivery
Authors
KeywordsDelivery
Folate conjugate
Gene transfection
Nucleic acids
Phosphorylcholine
Issue Date2009
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/1061186x.asp
Citation
Journal Of Drug Targeting, 2009, v. 17 n. 7, p. 512-523 How to Cite?
AbstractFolic acid has been investigated as a targeting ligand for imaging and therapeutic agent for over a decade; however, studies on its use in targeting of nonviral gene or nucleic acids delivery systems are sparse. This study assesses potential application of a new folic acid conjugate with aminomethacrylatephosphoryl-choline based copolymer (DMAEMA-MPCFA) as a targeting gene delivery vector. The folate-conjugated polymers produce colloidally stable polyplexes with a particle size <200nm and demonstrate the ability to protect DNA from enzymatic degradation to a certain extent. In cells that overexpress folate receptors (MCF-7 and KB cultures), the conjugated systems show a folate-specific association and achieved significantly enhanced transfection efficiency, compared to the nonconjugated control, with a dramatically reduced nonspecific cellular association. The transfection enhancement is achieved without a corresponding increase in cellular association, suggesting that an internal cellular trafficking of folate-conjugated system may be altered, resulting in an increased transfection efficacy. In summary, a new folate-conjugated aminomethacrylatephosphorylcholine copolymer is capable of forming colloidal complexes with DNA, modulating their specific cell uptake and improving the level of cell transfection in folate expressing cells. © 2009 Informa UK Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/91756
ISSN
2015 Impact Factor: 2.821
2015 SCImago Journal Rankings: 0.749
ISI Accession Number ID
Funding AgencyGrant Number
Biocompatibles (UK)
University of Nottingham (UK)
Funding Information:

The authors would like to thank Biocompatibles (UK) and The University of Nottingham (UK) for funding.

References

 

DC FieldValueLanguage
dc.contributor.authorLam, JKWen_HK
dc.contributor.authorArmes, SPen_HK
dc.contributor.authorLewis, ALen_HK
dc.contributor.authorStolnik, Sen_HK
dc.date.accessioned2010-09-17T10:25:38Z-
dc.date.available2010-09-17T10:25:38Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Drug Targeting, 2009, v. 17 n. 7, p. 512-523en_HK
dc.identifier.issn1061-186Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/91756-
dc.description.abstractFolic acid has been investigated as a targeting ligand for imaging and therapeutic agent for over a decade; however, studies on its use in targeting of nonviral gene or nucleic acids delivery systems are sparse. This study assesses potential application of a new folic acid conjugate with aminomethacrylatephosphoryl-choline based copolymer (DMAEMA-MPCFA) as a targeting gene delivery vector. The folate-conjugated polymers produce colloidally stable polyplexes with a particle size <200nm and demonstrate the ability to protect DNA from enzymatic degradation to a certain extent. In cells that overexpress folate receptors (MCF-7 and KB cultures), the conjugated systems show a folate-specific association and achieved significantly enhanced transfection efficiency, compared to the nonconjugated control, with a dramatically reduced nonspecific cellular association. The transfection enhancement is achieved without a corresponding increase in cellular association, suggesting that an internal cellular trafficking of folate-conjugated system may be altered, resulting in an increased transfection efficacy. In summary, a new folate-conjugated aminomethacrylatephosphorylcholine copolymer is capable of forming colloidal complexes with DNA, modulating their specific cell uptake and improving the level of cell transfection in folate expressing cells. © 2009 Informa UK Ltd.en_HK
dc.languageengen_HK
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/1061186x.aspen_HK
dc.relation.ispartofJournal of Drug Targetingen_HK
dc.subjectDeliveryen_HK
dc.subjectFolate conjugateen_HK
dc.subjectGene transfectionen_HK
dc.subjectNucleic acidsen_HK
dc.subjectPhosphorylcholineen_HK
dc.titleFolate conjugated phosphorylcholine-based polycations for specific targeting in nucleic acids deliveryen_HK
dc.typeArticleen_HK
dc.identifier.emailLam, JKW: jkwlam@hku.hken_HK
dc.identifier.authorityLam, JKW=rp01346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1080/10611860903023312en_HK
dc.identifier.pmid19534582-
dc.identifier.scopuseid_2-s2.0-70349786067en_HK
dc.identifier.hkuros176481-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70349786067&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume17en_HK
dc.identifier.issue7en_HK
dc.identifier.spage512en_HK
dc.identifier.epage523en_HK
dc.identifier.eissn1029-2330-
dc.identifier.isiWOS:000269549200004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLam, JKW=8404243000en_HK
dc.identifier.scopusauthoridArmes, SP=7103125898en_HK
dc.identifier.scopusauthoridLewis, AL=35305215700en_HK
dc.identifier.scopusauthoridStolnik, S=35515143300en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats