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Article: Viral load in patients infected with pandemic H1N1 2009 influenza A virus

TitleViral load in patients infected with pandemic H1N1 2009 influenza A virus
Authors
KeywordsPandemic
Serial
Stool
Urine
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
Citation
Journal Of Medical Virology, 2010, v. 82 n. 1, p. 1-7 How to Cite?
AbstractViral shedding profile of infections caused by the pandemic H1N1 2009 influenza A virus has not been reported. The aim of this study was to determine the viral load in different body sites. Viral loads of pandemic H1N1 virus in respiratory specimens, stool, urine, and serum were determined by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Respiratory specimens from patients with seasonal influenza were used as historical controls. Initial pre-treatment viral load were compared between these two groups. Serial respiratory specimens from patients with pandemic H1N1 virus infection were obtained for analysis of viral dynamics. Twenty-two pandemic H1N1 cases and 44 seasonal influenza historical controls were included. The mean initial viral load before oseltamivir therapy was 1.84 x 108 copies/ml for pandemic H1N1virus compared with 3.28 x 108 copies/ml in seasonal influenza historical controls (P=0.085). Among patients with pandemic H1N1 virus infection, peak viral load occurred on the day of onset of symptoms, and declined gradually afterwards, with no virus being detectable in respiratory specimens by RT-PCR 8 days and by culture 5 days after the onset of symptoms respectively, except in one patient. Pandemic H1N1 virus was detected in stool and in urine from 4/9 and 1/14 patients, respectively. Viral culture was also positive from the stool sample with the highest viral load. Younger age was associated with prolonged shedding in the respiratory tract and higher viral load in the stool. Data from this quantitative analysis of viral shedding may have implications for formulating infection control measures. © 2009 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/91714
ISSN
2014 Impact Factor: 2.347
ISI Accession Number ID
Funding AgencyGrant Number
Providence Foundation Limited
Hong Kong SAR Government
Funding Information:

Grant sponsor: Providence Foundation Limited in memory of the late Dr. Lui Hac Minh and the University Grant Council Research Fund for the Control of Infectious Diseases (RFCID) of the Food and Health Bureau of the Hong Kong SAR Government.

References

 

DC FieldValueLanguage
dc.contributor.authorTo, KKWen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorLi, IWSen_HK
dc.contributor.authorTsang, TYen_HK
dc.contributor.authorTse, Hen_HK
dc.contributor.authorChan, JFWen_HK
dc.contributor.authorHung, IFNen_HK
dc.contributor.authorLai, STen_HK
dc.contributor.authorLeung, CWen_HK
dc.contributor.authorKwan, YWen_HK
dc.contributor.authorLau, YLen_HK
dc.contributor.authorNg, TKen_HK
dc.contributor.authorCheng, VCCen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-17T10:24:09Z-
dc.date.available2010-09-17T10:24:09Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Medical Virology, 2010, v. 82 n. 1, p. 1-7en_HK
dc.identifier.issn0146-6615en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91714-
dc.description.abstractViral shedding profile of infections caused by the pandemic H1N1 2009 influenza A virus has not been reported. The aim of this study was to determine the viral load in different body sites. Viral loads of pandemic H1N1 virus in respiratory specimens, stool, urine, and serum were determined by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Respiratory specimens from patients with seasonal influenza were used as historical controls. Initial pre-treatment viral load were compared between these two groups. Serial respiratory specimens from patients with pandemic H1N1 virus infection were obtained for analysis of viral dynamics. Twenty-two pandemic H1N1 cases and 44 seasonal influenza historical controls were included. The mean initial viral load before oseltamivir therapy was 1.84 x 108 copies/ml for pandemic H1N1virus compared with 3.28 x 108 copies/ml in seasonal influenza historical controls (P=0.085). Among patients with pandemic H1N1 virus infection, peak viral load occurred on the day of onset of symptoms, and declined gradually afterwards, with no virus being detectable in respiratory specimens by RT-PCR 8 days and by culture 5 days after the onset of symptoms respectively, except in one patient. Pandemic H1N1 virus was detected in stool and in urine from 4/9 and 1/14 patients, respectively. Viral culture was also positive from the stool sample with the highest viral load. Younger age was associated with prolonged shedding in the respiratory tract and higher viral load in the stool. Data from this quantitative analysis of viral shedding may have implications for formulating infection control measures. © 2009 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763en_HK
dc.relation.ispartofJournal of Medical Virologyen_HK
dc.subjectPandemicen_HK
dc.subjectSerialen_HK
dc.subjectStoolen_HK
dc.subjectUrineen_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshChilden_HK
dc.subject.meshChild, Preschoolen_HK
dc.subject.meshChina - epidemiologyen_HK
dc.subject.meshDisease Outbreaks - statistics & numerical dataen_HK
dc.subject.meshFeces - virologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInfanten_HK
dc.subject.meshInfluenza A Virus, H1N1 Subtype - genetics - isolation & purification - physiologyen_HK
dc.subject.meshInfluenza, Human - epidemiology - virologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshRespiratory System - virologyen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshSeasonsen_HK
dc.subject.meshUrine - virologyen_HK
dc.subject.meshViral Load - physiologyen_HK
dc.subject.meshVirus Cultivationen_HK
dc.subject.meshVirus Sheddingen_HK
dc.subject.meshYoung Adulten_HK
dc.titleViral load in patients infected with pandemic H1N1 2009 influenza A virusen_HK
dc.typeArticleen_HK
dc.identifier.emailTo, KKW: kelvinto@hkucc.hku.hken_HK
dc.identifier.emailTse, H: htse@hkucc.hku.hken_HK
dc.identifier.emailChan, JFW: jfwchan@hku.hken_HK
dc.identifier.emailHung, IFN: ivanhung@hkucc.hku.hken_HK
dc.identifier.emailLau, YL: lauylung@hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityTo, KKW=rp01384en_HK
dc.identifier.authorityTse, H=rp00519en_HK
dc.identifier.authorityChan, JFW=rp01736en_HK
dc.identifier.authorityHung, IFN=rp00508en_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jmv.21664en_HK
dc.identifier.pmid19950247en_HK
dc.identifier.scopuseid_2-s2.0-74849115667en_HK
dc.identifier.hkuros173901-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-74849115667&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume82en_HK
dc.identifier.issue1en_HK
dc.identifier.spage1en_HK
dc.identifier.epage7en_HK
dc.identifier.eissn1096-9071-
dc.identifier.isiWOS:000272813000001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTo, KKW=14323807300en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridLi, IWS=24464179500en_HK
dc.identifier.scopusauthoridTsang, TY=7101832362en_HK
dc.identifier.scopusauthoridTse, H=7006070596en_HK
dc.identifier.scopusauthoridChan, JFW=24278817900en_HK
dc.identifier.scopusauthoridHung, IFN=7006103457en_HK
dc.identifier.scopusauthoridLai, ST=7402937038en_HK
dc.identifier.scopusauthoridLeung, CW=7402612619en_HK
dc.identifier.scopusauthoridKwan, YW=8596853200en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK
dc.identifier.scopusauthoridNg, TK=7402229817en_HK
dc.identifier.scopusauthoridCheng, VCC=23670479400en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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