Article: Viral load in patients infected with pandemic H1N1 2009 influenza A virus
| Title | Viral load in patients infected with pandemic H1N1 2009 influenza A virus | ||||||
|---|---|---|---|---|---|---|---|
| Authors | To, KKW Chan, KH Li, IWS Tsang, TY Tse, H Chan, JFW Hung, IFN Lai, ST Leung, CW Kwan, YW Lau, YL Ng, TK Cheng, VCC Peiris, JSM Yuen, KY | ||||||
| Keywords | Pandemic Serial Stool Urine | ||||||
| Issue Date | 2010 | ||||||
| Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763 | ||||||
| Citation | Journal Of Medical Virology, 2010, v. 82 n. 1, p. 1-7 [How to Cite?] DOI: http://dx.doi.org/10.1002/jmv.21664 | ||||||
| Abstract | Viral shedding profile of infections caused by the pandemic H1N1 2009 influenza A virus has not been reported. The aim of this study was to determine the viral load in different body sites. Viral loads of pandemic H1N1 virus in respiratory specimens, stool, urine, and serum were determined by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Respiratory specimens from patients with seasonal influenza were used as historical controls. Initial pre-treatment viral load were compared between these two groups. Serial respiratory specimens from patients with pandemic H1N1 virus infection were obtained for analysis of viral dynamics. Twenty-two pandemic H1N1 cases and 44 seasonal influenza historical controls were included. The mean initial viral load before oseltamivir therapy was 1.84 x 108 copies/ml for pandemic H1N1virus compared with 3.28 x 108 copies/ml in seasonal influenza historical controls (P=0.085). Among patients with pandemic H1N1 virus infection, peak viral load occurred on the day of onset of symptoms, and declined gradually afterwards, with no virus being detectable in respiratory specimens by RT-PCR 8 days and by culture 5 days after the onset of symptoms respectively, except in one patient. Pandemic H1N1 virus was detected in stool and in urine from 4/9 and 1/14 patients, respectively. Viral culture was also positive from the stool sample with the highest viral load. Younger age was associated with prolonged shedding in the respiratory tract and higher viral load in the stool. Data from this quantitative analysis of viral shedding may have implications for formulating infection control measures. © 2009 Wiley-Liss, Inc. | ||||||
| ISSN | 0146-6615 2011 Impact Factor: 2.82 2011 SCImago Journal Rankings: 0.267 | ||||||
| DOI | http://dx.doi.org/10.1002/jmv.21664 | ||||||
| ISI Accession Number ID | WOS:000272813000001
Funding Information: Grant sponsor: Providence Foundation Limited in memory of the late Dr. Lui Hac Minh and the University Grant Council Research Fund for the Control of Infectious Diseases (RFCID) of the Food and Health Bureau of the Hong Kong SAR Government. | ||||||
| References | References in Scopus |
| dc.contributor.author | To, KKW | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Chan, KH | ||||||
| dc.contributor.author | Li, IWS | ||||||
| dc.contributor.author | Tsang, TY | ||||||
| dc.contributor.author | Tse, H | ||||||
| dc.contributor.author | Chan, JFW | ||||||
| dc.contributor.author | Hung, IFN | ||||||
| dc.contributor.author | Lai, ST | ||||||
| dc.contributor.author | Leung, CW | ||||||
| dc.contributor.author | Kwan, YW | ||||||
| dc.contributor.author | Lau, YL | ||||||
| dc.contributor.author | Ng, TK | ||||||
| dc.contributor.author | Cheng, VCC | ||||||
| dc.contributor.author | Peiris, JSM | ||||||
| dc.contributor.author | Yuen, KY | ||||||
| dc.date.accessioned | 2010-09-17T10:24:09Z | ||||||
| dc.date.available | 2010-09-17T10:24:09Z | ||||||
| dc.date.issued | 2010 | ||||||
| dc.description.abstract | Viral shedding profile of infections caused by the pandemic H1N1 2009 influenza A virus has not been reported. The aim of this study was to determine the viral load in different body sites. Viral loads of pandemic H1N1 virus in respiratory specimens, stool, urine, and serum were determined by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Respiratory specimens from patients with seasonal influenza were used as historical controls. Initial pre-treatment viral load were compared between these two groups. Serial respiratory specimens from patients with pandemic H1N1 virus infection were obtained for analysis of viral dynamics. Twenty-two pandemic H1N1 cases and 44 seasonal influenza historical controls were included. The mean initial viral load before oseltamivir therapy was 1.84 x 108 copies/ml for pandemic H1N1virus compared with 3.28 x 108 copies/ml in seasonal influenza historical controls (P=0.085). Among patients with pandemic H1N1 virus infection, peak viral load occurred on the day of onset of symptoms, and declined gradually afterwards, with no virus being detectable in respiratory specimens by RT-PCR 8 days and by culture 5 days after the onset of symptoms respectively, except in one patient. Pandemic H1N1 virus was detected in stool and in urine from 4/9 and 1/14 patients, respectively. Viral culture was also positive from the stool sample with the highest viral load. Younger age was associated with prolonged shedding in the respiratory tract and higher viral load in the stool. Data from this quantitative analysis of viral shedding may have implications for formulating infection control measures. © 2009 Wiley-Liss, Inc. | ||||||
| dc.description.nature | link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Journal Of Medical Virology, 2010, v. 82 n. 1, p. 1-7 [How to Cite?] DOI: http://dx.doi.org/10.1002/jmv.21664 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1002/jmv.21664 | ||||||
| dc.identifier.eissn | 1096-9071 | ||||||
| dc.identifier.epage | 7 | ||||||
| dc.identifier.hkuros | 173901 | ||||||
| dc.identifier.isi | WOS:000272813000001
Funding Information: Grant sponsor: Providence Foundation Limited in memory of the late Dr. Lui Hac Minh and the University Grant Council Research Fund for the Control of Infectious Diseases (RFCID) of the Food and Health Bureau of the Hong Kong SAR Government. | ||||||
| dc.identifier.issn | 0146-6615 2011 Impact Factor: 2.82 2011 SCImago Journal Rankings: 0.267 | ||||||
| dc.identifier.issue | 1 | ||||||
| dc.identifier.pmid | 19950247 | ||||||
| dc.identifier.scopus | eid_2-s2.0-74849115667 | ||||||
| dc.identifier.spage | 1 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/91714 | ||||||
| dc.identifier.volume | 82 | ||||||
| dc.language | eng | ||||||
| dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763 | ||||||
| dc.publisher.place | United States | ||||||
| dc.relation.ispartof | Journal of Medical Virology | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject.mesh | Adolescent | ||||||
| dc.subject.mesh | Adult | ||||||
| dc.subject.mesh | Child | ||||||
| dc.subject.mesh | Child, Preschool | ||||||
| dc.subject.mesh | China - epidemiology | ||||||
| dc.subject.mesh | Disease Outbreaks - statistics & numerical data | ||||||
| dc.subject.mesh | Feces - virology | ||||||
| dc.subject.mesh | Female | ||||||
| dc.subject.mesh | Humans | ||||||
| dc.subject.mesh | Infant | ||||||
| dc.subject.mesh | Influenza A Virus, H1N1 Subtype - genetics - isolation & purification - physiology | ||||||
| dc.subject.mesh | Influenza, Human - epidemiology - virology | ||||||
| dc.subject.mesh | Male | ||||||
| dc.subject.mesh | Middle Aged | ||||||
| dc.subject.mesh | Respiratory System - virology | ||||||
| dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | ||||||
| dc.subject.mesh | Seasons | ||||||
| dc.subject.mesh | Urine - virology | ||||||
| dc.subject.mesh | Viral Load - physiology | ||||||
| dc.subject.mesh | Virus Cultivation | ||||||
| dc.subject.mesh | Virus Shedding | ||||||
| dc.subject.mesh | Young Adult | ||||||
| dc.subject | Pandemic | ||||||
| dc.subject | Serial | ||||||
| dc.subject | Stool | ||||||
| dc.subject | Urine | ||||||
| dc.title | Viral load in patients infected with pandemic H1N1 2009 influenza A virus | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong
- Princess Margaret Hospital Hong Kong