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Article: Secreted PDZD2 exerts concentration-dependent effects on the proliferation of INS-1E cells

TitleSecreted PDZD2 exerts concentration-dependent effects on the proliferation of INS-1E cells
Authors
KeywordsINS-1E
Insulin
Pancreas
PDZD2
sPDZD2
Issue Date2006
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/biocel
Citation
International Journal Of Biochemistry And Cell Biology, 2006, v. 38 n. 5-6, p. 1015-1022 How to Cite?
AbstractPDZD2 (PDZ domain containing 2) is a multi-PDZ protein expressed in pancreas and many other tissues. PDZD2 shows extensive homology to pro-interleukin-16 (pro-IL-16) and is localized mainly to the endoplasmic reticulum. We have recently demonstrated that PDZD2, like pro-IL-16, is proteolytically cleaved at its C-terminus to generate a secreted protein, sPDZD2 (for secreted PDZD2). To understand the possible functional role of PDZD2 in pancreas, we investigated the cellular distribution of PDZD2 in adult pancreas using an antiserum that recognizes both the full-length and secreted forms of PDZD2. Immunohistochemical analysis revealed a strong expression of PDZD2 in pancreatic islet β cells but not α cells. Consistent with the β-cell-enriched expression of PDZD2, immunoblot analysis indicated expression of both full-length PDZD2 and sPDZD2 in the insulinoma cell line INS-1E. A recombinant sPDZD2 protein was synthesized for study of its functional effect on INS-1E cells. In culture media with limiting serum, co-incubation with sPDZD2 stimulated the proliferation of INS-1E cells. The mitogenic effect of sPDZD2 was concentration-dependent, and was associated with a slight inhibition of the insulin promoter activity at high sPDZD2 concentrations. As a potential mitogen of β-like cells, sPDZD2 may be useful for the optimization of β-cell growth and differentiation in vitro. © 2005 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/91712
ISSN
2015 Impact Factor: 3.905
2015 SCImago Journal Rankings: 2.003
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMa, RYMen_HK
dc.contributor.authorTam, TSMen_HK
dc.contributor.authorSuen, APMen_HK
dc.contributor.authorYeung, PMLen_HK
dc.contributor.authorTsang, SWen_HK
dc.contributor.authorChung, SKen_HK
dc.contributor.authorThomas, MKen_HK
dc.contributor.authorLeung, PSen_HK
dc.contributor.authorYao, KMen_HK
dc.date.accessioned2010-09-17T10:24:05Z-
dc.date.available2010-09-17T10:24:05Z-
dc.date.issued2006en_HK
dc.identifier.citationInternational Journal Of Biochemistry And Cell Biology, 2006, v. 38 n. 5-6, p. 1015-1022en_HK
dc.identifier.issn1357-2725en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91712-
dc.description.abstractPDZD2 (PDZ domain containing 2) is a multi-PDZ protein expressed in pancreas and many other tissues. PDZD2 shows extensive homology to pro-interleukin-16 (pro-IL-16) and is localized mainly to the endoplasmic reticulum. We have recently demonstrated that PDZD2, like pro-IL-16, is proteolytically cleaved at its C-terminus to generate a secreted protein, sPDZD2 (for secreted PDZD2). To understand the possible functional role of PDZD2 in pancreas, we investigated the cellular distribution of PDZD2 in adult pancreas using an antiserum that recognizes both the full-length and secreted forms of PDZD2. Immunohistochemical analysis revealed a strong expression of PDZD2 in pancreatic islet β cells but not α cells. Consistent with the β-cell-enriched expression of PDZD2, immunoblot analysis indicated expression of both full-length PDZD2 and sPDZD2 in the insulinoma cell line INS-1E. A recombinant sPDZD2 protein was synthesized for study of its functional effect on INS-1E cells. In culture media with limiting serum, co-incubation with sPDZD2 stimulated the proliferation of INS-1E cells. The mitogenic effect of sPDZD2 was concentration-dependent, and was associated with a slight inhibition of the insulin promoter activity at high sPDZD2 concentrations. As a potential mitogen of β-like cells, sPDZD2 may be useful for the optimization of β-cell growth and differentiation in vitro. © 2005 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/biocelen_HK
dc.relation.ispartofInternational Journal of Biochemistry and Cell Biologyen_HK
dc.subjectINS-1Een_HK
dc.subjectInsulinen_HK
dc.subjectPancreasen_HK
dc.subjectPDZD2en_HK
dc.subjectsPDZD2en_HK
dc.subject.meshAdaptor Proteins, Signal Transducingen_HK
dc.subject.meshAdulten_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshCell Proliferation - drug effectsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInsulin-Secreting Cells - metabolismen_HK
dc.subject.meshIntracellular Signaling Peptides and Proteins - physiology - secretionen_HK
dc.subject.meshMitogens - pharmacologyen_HK
dc.subject.meshNeoplasm Proteinsen_HK
dc.subject.meshPancreas - metabolismen_HK
dc.titleSecreted PDZD2 exerts concentration-dependent effects on the proliferation of INS-1E cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailYeung, PML:pmlyeung@hku.hken_HK
dc.identifier.emailChung, SK:skchung@hkucc.hku.hken_HK
dc.identifier.emailYao, KM:kmyao@hku.hken_HK
dc.identifier.authorityYeung, PML=rp01402en_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.identifier.authorityYao, KM=rp00344en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.biocel.2005.11.012en_HK
dc.identifier.pmid16413998-
dc.identifier.scopuseid_2-s2.0-33644836284en_HK
dc.identifier.hkuros119609-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33644836284&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume38en_HK
dc.identifier.issue5-6en_HK
dc.identifier.spage1015en_HK
dc.identifier.epage1022en_HK
dc.identifier.isiWOS:000236525800031-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridMa, RYM=8323783700en_HK
dc.identifier.scopusauthoridTam, TSM=12772669500en_HK
dc.identifier.scopusauthoridSuen, APM=12773296100en_HK
dc.identifier.scopusauthoridYeung, PML=8350940900en_HK
dc.identifier.scopusauthoridTsang, SW=8050597200en_HK
dc.identifier.scopusauthoridChung, SK=7404292976en_HK
dc.identifier.scopusauthoridThomas, MK=7404754193en_HK
dc.identifier.scopusauthoridLeung, PS=7401748938en_HK
dc.identifier.scopusauthoridYao, KM=7403234578en_HK

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