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Article: Measurement of anti-dsDNA: A comparative study of two ELISA and the Crithidia assay

TitleMeasurement of anti-dsDNA: A comparative study of two ELISA and the Crithidia assay
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date1998
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00313025.asp
Citation
Pathology, 1998, v. 30 n. 1, p. 57-61 How to Cite?
AbstractWe compared the measurement of anti-dsDNA by a commercial ELISA test (DIASTAT), an in-house ELISA and the Crithidia luciliae assay in cross-sectional sera samples of 209 systemic lupus erythematosus (SLE) patients and 64 patients with a variety of rheumatological, autoimmune and non-autoimmune diseases in Hong Kong. The Crithidia assay was found to be the least sensitive (17%) but most specific (95%) method for detection of a positive result in SLE patients. The DIASTAT assay has a higher sensitivity (68%) but lower specificity (80%) than the in-house ELISA test (32% sensitivity and 89% specificity). The positive predictive value of the three assays are comparable at 90-92% while DIASTAT had the highest negative predictive value (44%). There was good linear correlation (r = 0.7) between the two ELISAs. ELISA can serve as a useful screening test for anti-dsDNA in SLE patients and doubtful cases can then be confirmed by another method such as radioimmunoassay.
Persistent Identifierhttp://hdl.handle.net/10722/91628
ISSN
2015 Impact Factor: 2.968
2015 SCImago Journal Rankings: 1.049
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, KHen_HK
dc.contributor.authorLawton, JWMen_HK
dc.contributor.authorCheng, SKLen_HK
dc.contributor.authorLee, SSen_HK
dc.contributor.authorLau, CSen_HK
dc.date.accessioned2010-09-17T10:22:26Z-
dc.date.available2010-09-17T10:22:26Z-
dc.date.issued1998en_HK
dc.identifier.citationPathology, 1998, v. 30 n. 1, p. 57-61en_HK
dc.identifier.issn0031-3025en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91628-
dc.description.abstractWe compared the measurement of anti-dsDNA by a commercial ELISA test (DIASTAT), an in-house ELISA and the Crithidia luciliae assay in cross-sectional sera samples of 209 systemic lupus erythematosus (SLE) patients and 64 patients with a variety of rheumatological, autoimmune and non-autoimmune diseases in Hong Kong. The Crithidia assay was found to be the least sensitive (17%) but most specific (95%) method for detection of a positive result in SLE patients. The DIASTAT assay has a higher sensitivity (68%) but lower specificity (80%) than the in-house ELISA test (32% sensitivity and 89% specificity). The positive predictive value of the three assays are comparable at 90-92% while DIASTAT had the highest negative predictive value (44%). There was good linear correlation (r = 0.7) between the two ELISAs. ELISA can serve as a useful screening test for anti-dsDNA in SLE patients and doubtful cases can then be confirmed by another method such as radioimmunoassay.en_HK
dc.languageengen_HK
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00313025.aspen_HK
dc.relation.ispartofPathologyen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntibodies, Antinuclear - blood - immunologyen_HK
dc.subject.meshArthritis, Rheumatoid - blood - immunologyen_HK
dc.subject.meshAutoantibodies - blood - immunologyen_HK
dc.subject.meshAutoimmune Diseases - blood - immunologyen_HK
dc.subject.meshBiological Assay - methodsen_HK
dc.subject.meshCrithidia - chemistry - immunologyen_HK
dc.subject.meshData Interpretation, Statisticalen_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assay - methodsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLupus Erythematosus, Systemic - blood - immunologyen_HK
dc.subject.meshPredictive Value of Testsen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.subject.meshSkin Diseases - blood - immunologyen_HK
dc.titleMeasurement of anti-dsDNA: A comparative study of two ELISA and the Crithidia assayen_HK
dc.typeArticleen_HK
dc.identifier.emailLau, CS:cslau@hku.hken_HK
dc.identifier.authorityLau, CS=rp01348en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1080/00313029800169675en_HK
dc.identifier.pmid9534209-
dc.identifier.scopuseid_2-s2.0-0031896031en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031896031&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue1en_HK
dc.identifier.spage57en_HK
dc.identifier.epage61en_HK
dc.identifier.isiWOS:000072358700012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWong, KH=36485841700en_HK
dc.identifier.scopusauthoridLawton, JWM=7102180792en_HK
dc.identifier.scopusauthoridCheng, SKL=36856417300en_HK
dc.identifier.scopusauthoridLee, SS=7601394467en_HK
dc.identifier.scopusauthoridLau, CS=14035682100en_HK

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