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Article: Functional consequences of overexpressing the gap junction Cx43 in the cardiogenic potential of pluripotent human embryonic stem cells

TitleFunctional consequences of overexpressing the gap junction Cx43 in the cardiogenic potential of pluripotent human embryonic stem cells
Authors
KeywordsCardiac differentiation
Connexin
Human embryonic stem cells
Pluripotency
Issue Date2008
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2008, v. 377 n. 1, p. 46-51 How to Cite?
AbstractGap junctions, encoded by the connexin (Cx) multi-gene family, couple adjacent cells and underlie cell-cell communications. Previous mouse studies suggest that Cxs play an important role in development but their role in human cardiogenesis is undefined. Human embryonic stem cells (hESC) provide a unique model for studying human differentiation. Lentivirus-mediated stable overexpression of Cx43 in hESC (Cx43-hESC) did not affect colony morphology, karyotype and expression of pluripotency genes such as Oct4 but completely suppressed the formation of spontaneously beating, cardiomyocyte-containing clusters in embryoid bodies (EBs). Unlike control hEBs, the transcripts of several mesodermal markers (kallikrein, δ-globin, and CMP), ventricular myosin light chain and cardiac troponin I were absent or delayed. Transcriptomic and pathway analyses showed that 194 genes crucial for movement, growth, differentiation and maintenance were differentially expressed in Cx43-hESC. We conclude that Cx43 mediates the expression of an array of genes involved in human cardiogenesis, in addition to intercellular communication. © 2008 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/91625
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
Funding AgencyGrant Number
National Institutes of HealthR01 HL-72857
F32 HL078330
Hong Kong Research Grant CouncilHKU 7633/06M
Funding Information:

This work was supported by grants from the National Institutes of Health (R01 HL-72857 to R.A.L. and F32 HL078330 to J.C.M) and the Hong Kong Research Grant Council (HKU 7633/06M to H.F.T., and R.A.L).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorMoore, JCen_HK
dc.contributor.authorTsang, SYen_HK
dc.contributor.authorRushing, SNen_HK
dc.contributor.authorLin, Den_HK
dc.contributor.authorTse, HFen_HK
dc.contributor.authorChan, CWYen_HK
dc.contributor.authorLi, RAen_HK
dc.date.accessioned2010-09-17T10:22:24Z-
dc.date.available2010-09-17T10:22:24Z-
dc.date.issued2008en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2008, v. 377 n. 1, p. 46-51en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/91625-
dc.description.abstractGap junctions, encoded by the connexin (Cx) multi-gene family, couple adjacent cells and underlie cell-cell communications. Previous mouse studies suggest that Cxs play an important role in development but their role in human cardiogenesis is undefined. Human embryonic stem cells (hESC) provide a unique model for studying human differentiation. Lentivirus-mediated stable overexpression of Cx43 in hESC (Cx43-hESC) did not affect colony morphology, karyotype and expression of pluripotency genes such as Oct4 but completely suppressed the formation of spontaneously beating, cardiomyocyte-containing clusters in embryoid bodies (EBs). Unlike control hEBs, the transcripts of several mesodermal markers (kallikrein, δ-globin, and CMP), ventricular myosin light chain and cardiac troponin I were absent or delayed. Transcriptomic and pathway analyses showed that 194 genes crucial for movement, growth, differentiation and maintenance were differentially expressed in Cx43-hESC. We conclude that Cx43 mediates the expression of an array of genes involved in human cardiogenesis, in addition to intercellular communication. © 2008 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectCardiac differentiationen_HK
dc.subjectConnexinen_HK
dc.subjectHuman embryonic stem cellsen_HK
dc.subjectPluripotencyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Communication - geneticsen_HK
dc.subject.meshCell Differentiation - geneticsen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshConnexin 43 - genetics - metabolismen_HK
dc.subject.meshEmbryonic Stem Cells - cytology - metabolism - physiologyen_HK
dc.subject.meshGap Junctions - metabolismen_HK
dc.subject.meshGene Expression Regulation, Developmentalen_HK
dc.subject.meshHeart - embryologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMyocytes, Cardiac - cytologyen_HK
dc.subject.meshOrganogenesis - geneticsen_HK
dc.subject.meshPluripotent Stem Cells - cytology - metabolism - physiologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshTranscription, Geneticen_HK
dc.titleFunctional consequences of overexpressing the gap junction Cx43 in the cardiogenic potential of pluripotent human embryonic stem cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailTse, HF:hftse@hkucc.hku.hken_HK
dc.identifier.emailChan, CWY:camchan@hku.hken_HK
dc.identifier.emailLi, RA:ronaldli@hkucc.hku.hken_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.identifier.authorityChan, CWY=rp01311en_HK
dc.identifier.authorityLi, RA=rp01352en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbrc.2008.09.076en_HK
dc.identifier.pmid18823947-
dc.identifier.scopuseid_2-s2.0-54849442444en_HK
dc.identifier.hkuros158692-
dc.identifier.hkuros222566-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-54849442444&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume377en_HK
dc.identifier.issue1en_HK
dc.identifier.spage46en_HK
dc.identifier.epage51en_HK
dc.identifier.eissn1090-2104-
dc.identifier.isiWOS:000260738500009-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectGenetic enrichment of cardiac derivatives from human embryonic stem cells and their bioengineering for cell-based heart therapies-
dc.identifier.scopusauthoridMoore, JC=35185459800en_HK
dc.identifier.scopusauthoridTsang, SY=7102255908en_HK
dc.identifier.scopusauthoridRushing, SN=25121769500en_HK
dc.identifier.scopusauthoridLin, D=8905703100en_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK
dc.identifier.scopusauthoridChan, CWY=12240386600en_HK
dc.identifier.scopusauthoridLi, RA=7404724466en_HK
dc.identifier.citeulike6254615-

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