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Article: Adrenomedullin is both proinflammatory and antiinflammatory: Its effects on gene expression and secretion of cytokines and macrophage migration inhibitory factor in NR8383 macrophage cell line

TitleAdrenomedullin is both proinflammatory and antiinflammatory: Its effects on gene expression and secretion of cytokines and macrophage migration inhibitory factor in NR8383 macrophage cell line
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2005
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2005, v. 146 n. 3, p. 1321-1327 How to Cite?
AbstractAdrenomedullin (ADM) is a potent vasorelaxant peptide that plays important roles in cardiovascular homeostasis and inflammatory response. ADM derived from macrophages is one of the major sources of ADM that is produced in the inflammatory process. To assess the functions of ADM in inflammation, we studied the temporal changes in ADM production and its effect on secretion of macrophage migration inhibitory factor (MIF) and cytokine response of NR8383 rat macrophages activated by lipopolysaccharide (LPS). NR8383 cells were stimulated by LPS in the absence and presence of exogenous ADM, and the concentrations of ADM, MIF, and proinflammatory cytokines (IL-6, TNF-α, and IL-1β) in the culture media and gene expressions of the cells were measured. We confirmed that the secretion and mRNA expression of ADM in the macrophages were markedly increased by LPS. ADM increased initial secretion of MIF and IL-1β from both nonstimulated and LPS-stimulated cells, and it also increased basal and LPS-induced IL-6 secretion of the cells by 2- to 15-fold. However, it reduced secretion of TNF-α from LPS-stimulated cells by 34-56%. Our results suggest that ADM modulates MIF secretion and cytokine production and plays important roles in both the initiation and propagation of the inflammatory response.
Persistent Identifierhttp://hdl.handle.net/10722/91617
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, LYFen_HK
dc.contributor.authorCheung, BMYen_HK
dc.contributor.authorLi, YYen_HK
dc.contributor.authorTang, Fen_HK
dc.date.accessioned2010-09-17T10:22:16Z-
dc.date.available2010-09-17T10:22:16Z-
dc.date.issued2005en_HK
dc.identifier.citationEndocrinology, 2005, v. 146 n. 3, p. 1321-1327en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91617-
dc.description.abstractAdrenomedullin (ADM) is a potent vasorelaxant peptide that plays important roles in cardiovascular homeostasis and inflammatory response. ADM derived from macrophages is one of the major sources of ADM that is produced in the inflammatory process. To assess the functions of ADM in inflammation, we studied the temporal changes in ADM production and its effect on secretion of macrophage migration inhibitory factor (MIF) and cytokine response of NR8383 rat macrophages activated by lipopolysaccharide (LPS). NR8383 cells were stimulated by LPS in the absence and presence of exogenous ADM, and the concentrations of ADM, MIF, and proinflammatory cytokines (IL-6, TNF-α, and IL-1β) in the culture media and gene expressions of the cells were measured. We confirmed that the secretion and mRNA expression of ADM in the macrophages were markedly increased by LPS. ADM increased initial secretion of MIF and IL-1β from both nonstimulated and LPS-stimulated cells, and it also increased basal and LPS-induced IL-6 secretion of the cells by 2- to 15-fold. However, it reduced secretion of TNF-α from LPS-stimulated cells by 34-56%. Our results suggest that ADM modulates MIF secretion and cytokine production and plays important roles in both the initiation and propagation of the inflammatory response.en_HK
dc.languageengen_HK
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.subject.meshAdrenomedullinen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAnti-Inflammatory Agents - pharmacologyen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCulture Media, Conditioned - pharmacologyen_HK
dc.subject.meshCytokines - biosynthesis - metabolismen_HK
dc.subject.meshDNA Primers - chemistryen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshEscherichia coli - metabolismen_HK
dc.subject.meshGene Expression Regulationen_HK
dc.subject.meshInflammationen_HK
dc.subject.meshInflammation Mediators - pharmacologyen_HK
dc.subject.meshInterleukin-1 - metabolismen_HK
dc.subject.meshInterleukin-6 - metabolismen_HK
dc.subject.meshLipopolysaccharides - chemistry - metabolismen_HK
dc.subject.meshMacrophage Migration-Inhibitory Factors - metabolismen_HK
dc.subject.meshMacrophages - metabolismen_HK
dc.subject.meshPeptides - metabolism - physiologyen_HK
dc.subject.meshRNA, Messenger - metabolismen_HK
dc.subject.meshRadioimmunoassayen_HK
dc.subject.meshRatsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshTime Factorsen_HK
dc.subject.meshTumor Necrosis Factor-alpha - metabolismen_HK
dc.titleAdrenomedullin is both proinflammatory and antiinflammatory: Its effects on gene expression and secretion of cytokines and macrophage migration inhibitory factor in NR8383 macrophage cell lineen_HK
dc.typeArticleen_HK
dc.identifier.emailCheung, BMY:mycheung@hku.hken_HK
dc.identifier.emailTang, F:ftang@hkucc.hku.hken_HK
dc.identifier.authorityCheung, BMY=rp01321en_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1210/en.2004-1080en_HK
dc.identifier.pmid15576460-
dc.identifier.scopuseid_2-s2.0-14244255855en_HK
dc.identifier.hkuros113709-
dc.identifier.hkuros105066-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-14244255855&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume146en_HK
dc.identifier.issue3en_HK
dc.identifier.spage1321en_HK
dc.identifier.epage1327en_HK
dc.identifier.isiWOS:000227035400040-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWong, LYF=24476809800en_HK
dc.identifier.scopusauthoridCheung, BMY=7103294806en_HK
dc.identifier.scopusauthoridLi, YY=8293496300en_HK
dc.identifier.scopusauthoridTang, F=7201979770en_HK

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