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Article: Hyperpolarization-activated cyclic nucleotide-gated channels in pancreatic β-cell

TitleHyperpolarization-activated cyclic nucleotide-gated channels in pancreatic β-cell
Authors
KeywordsSpecies Index: Rattus
Issue Date2007
PublisherEndocrine Society. The Journal's web site is located at http://mend.endojournals.org/
Citation
Molecular Endocrinology, 2007, v. 21 n. 3, p. 753-764 How to Cite?
AbstractHyperpolarization-activated cyclic nucleotide-modulated (HCN) channels mediate the pacemaker current (Ih or If) observed in electrically rhythmic cardiac and neuronal cells. Here we describe a hyperpolarization-activated time-dependent cationic current, β-I h, in pancreatic β-cells. Transcripts for HCN1-4 were detected by RT-PCR and quantitative PCR in rat islets and MIN6 mouse insulinoma cells. β-Ih in rat β-cells and MIN6 cells displayed biophysical and pharmacological properties similar to those of HCN currents in cardiac and neuronal cells. Stimulation of cAMP production with forskolin/3-isobutyl-1- methylxanthine (50 μM) or dibutyryl-cAMP (1 mM) caused a significant rightward shift in the midpoint activation potential of β-Ih, whereas expression of either specific small interfering (si)RNA against HCN2 (siHCN2b) or a dominant-negative HCN channel (HCN1-AAA) caused a near-complete inhibition of time-dependent β-Ih. However, expression of siHCN2b in MIN6 cells had no affect on glucose-stimulated insulin secretion under normal or cAMP-stimulated conditions. Blocking β-Ih in intact rat islets also did not affect membrane potential behavior at basal glucose concentrations. Taken together, our experiments provide the first evidence for functional expression of HCN channels in the pancreatic β-cell. Copyright © 2007 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/91558
ISSN
2015 Impact Factor: 3.432
2015 SCImago Journal Rankings: 2.195
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorElKholy, Wen_HK
dc.contributor.authorMacDonald, PEen_HK
dc.contributor.authorFox, JMen_HK
dc.contributor.authorBhattacharjee, Aen_HK
dc.contributor.authorXue, Ten_HK
dc.contributor.authorGao, Xen_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorStieber, Jen_HK
dc.contributor.authorLi, RAen_HK
dc.contributor.authorTsushima, RGen_HK
dc.contributor.authorWheeler, MBen_HK
dc.date.accessioned2010-09-17T10:21:20Z-
dc.date.available2010-09-17T10:21:20Z-
dc.date.issued2007en_HK
dc.identifier.citationMolecular Endocrinology, 2007, v. 21 n. 3, p. 753-764en_HK
dc.identifier.issn0888-8809en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91558-
dc.description.abstractHyperpolarization-activated cyclic nucleotide-modulated (HCN) channels mediate the pacemaker current (Ih or If) observed in electrically rhythmic cardiac and neuronal cells. Here we describe a hyperpolarization-activated time-dependent cationic current, β-I h, in pancreatic β-cells. Transcripts for HCN1-4 were detected by RT-PCR and quantitative PCR in rat islets and MIN6 mouse insulinoma cells. β-Ih in rat β-cells and MIN6 cells displayed biophysical and pharmacological properties similar to those of HCN currents in cardiac and neuronal cells. Stimulation of cAMP production with forskolin/3-isobutyl-1- methylxanthine (50 μM) or dibutyryl-cAMP (1 mM) caused a significant rightward shift in the midpoint activation potential of β-Ih, whereas expression of either specific small interfering (si)RNA against HCN2 (siHCN2b) or a dominant-negative HCN channel (HCN1-AAA) caused a near-complete inhibition of time-dependent β-Ih. However, expression of siHCN2b in MIN6 cells had no affect on glucose-stimulated insulin secretion under normal or cAMP-stimulated conditions. Blocking β-Ih in intact rat islets also did not affect membrane potential behavior at basal glucose concentrations. Taken together, our experiments provide the first evidence for functional expression of HCN channels in the pancreatic β-cell. Copyright © 2007 by The Endocrine Society.en_HK
dc.languageengen_HK
dc.publisherEndocrine Society. The Journal's web site is located at http://mend.endojournals.org/en_HK
dc.relation.ispartofMolecular Endocrinologyen_HK
dc.subjectSpecies Index: Rattusen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBenzazepines - pharmacologyen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCyclic AMP - physiologyen_HK
dc.subject.meshCyclic Nucleotide-Gated Cation Channelsen_HK
dc.subject.meshElectrophysiologyen_HK
dc.subject.meshExocytosis - drug effectsen_HK
dc.subject.meshInsulin - secretionen_HK
dc.subject.meshInsulin-Secreting Cells - drug effects - metabolism - physiologyen_HK
dc.subject.meshInsulinoma - pathologyen_HK
dc.subject.meshMembrane Potentials - drug effectsen_HK
dc.subject.meshMiceen_HK
dc.subject.meshPiperidines - pharmacologyen_HK
dc.subject.meshPotassium Channel Blockers - metabolismen_HK
dc.subject.meshPotassium Channels - metabolismen_HK
dc.subject.meshPyrimidines - pharmacologyen_HK
dc.subject.meshRNA, Small Interfering - pharmacologyen_HK
dc.subject.meshRatsen_HK
dc.titleHyperpolarization-activated cyclic nucleotide-gated channels in pancreatic β-cellen_HK
dc.typeArticleen_HK
dc.identifier.emailLi, RA:ronaldli@hkucc.hku.hken_HK
dc.identifier.authorityLi, RA=rp01352en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1210/me.2006-0258en_HK
dc.identifier.pmid17158221-
dc.identifier.scopuseid_2-s2.0-33847227276en_HK
dc.identifier.hkuros183051-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33847227276&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume21en_HK
dc.identifier.issue3en_HK
dc.identifier.spage753en_HK
dc.identifier.epage764en_HK
dc.identifier.isiWOS:000244407400014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridElKholy, W=6603643213en_HK
dc.identifier.scopusauthoridMacDonald, PE=7202038304en_HK
dc.identifier.scopusauthoridFox, JM=7404282239en_HK
dc.identifier.scopusauthoridBhattacharjee, A=15924959000en_HK
dc.identifier.scopusauthoridXue, T=7005064190en_HK
dc.identifier.scopusauthoridGao, X=7403872753en_HK
dc.identifier.scopusauthoridZhang, Y=8641344700en_HK
dc.identifier.scopusauthoridStieber, J=7006501938en_HK
dc.identifier.scopusauthoridLi, RA=7404724466en_HK
dc.identifier.scopusauthoridTsushima, RG=7006183117en_HK
dc.identifier.scopusauthoridWheeler, MB=7403129168en_HK

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