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Article: Differential induction of adrenomedullin, interleukins and tumour necrosis factor-α by lipopolysaccharide in rat tissues in vivo

TitleDifferential induction of adrenomedullin, interleukins and tumour necrosis factor-α by lipopolysaccharide in rat tissues in vivo
Authors
KeywordsAdrenal
Cytokines
Endotoxin
Kidney
Liver
Spleen
Issue Date2005
PublisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CEP
Citation
Clinical And Experimental Pharmacology And Physiology, 2005, v. 32 n. 12, p. 1110-1118 How to Cite?
Abstract1. The aim of the present study was to determine the temporal changes in tissue adrenomedullin (AM) and cytokine contents and cytokine and preproAM mRNA levels in the kidney, liver, adrenal gland and spleen of lipopolysaccharide (LPS)-treated rats. 2. Rats were injected with LPS (10 mg/kg, i.p.). Radioimmunoassay and solution hybridization-RNase protection assays were used to follow the changes in AM and its mRNA levels, respectively; ELISA and reverse transcription-polymerase chain reaction were used to follow the changes in cytokines and their mRNA levels, respectively. 3. In the kidney, the preproAM mRNA levels were increased 1 and 3 h after LPS treatment, whereas AM levels were decreased at 3 h. Interleukin (IL)-6 and IL-1β levels were increased at 3 and 6 h, respectively. 4. The preproAM mRNA levels were elevated in the liver 3 h after LPS injection. Concentrations of tumour necrosis factor (TNF)-α and IL-1β were increased at l and 6 h, respectively. 5. There were no changes in the levels of either preproAM mRNA or AM in the adrenal gland and the spleen. In the spleen, TNF-α levels were elevated at 1 and 3 h after LPS injection and IL-1β was elevated at 1 and 6 h after LPS injection, whereas in the adrenal gland IL-1β was elevated at 6 h after injection. 6. The mRNA levels of the three cytokines were elevated at all three time intervals examined in the kidney, liver, adrenal gland and spleen, with the exception that TNF-α mRNA was not elevated in the adrenal gland at 6 h after LPS injection and IL-1β mRNA was not elevated in the spleen at 3 and 6 h. 7. The plasma concentrations of TNF-α were increased at 1 and 3 h after LPS injection, whereas plasma concentration of IL-1β and IL-6 were elevated at 3 and 6 h for both. 8. The present results suggest that the biosynthesis and secretion of AM may be differentially regulated in various tissues of rats injected with LPS and that AM may interact with cytokines during inflammation. © 2005 Blackwell Publishing Asia Pty Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/91552
ISSN
2012 Impact Factor: 2.16
2015 SCImago Journal Rankings: 0.944
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, YYen_HK
dc.contributor.authorWong, LYen_HK
dc.contributor.authorCheung, BMen_HK
dc.contributor.authorHwang, ISen_HK
dc.contributor.authorTang, Fen_HK
dc.date.accessioned2010-09-17T10:21:15Z-
dc.date.available2010-09-17T10:21:15Z-
dc.date.issued2005en_HK
dc.identifier.citationClinical And Experimental Pharmacology And Physiology, 2005, v. 32 n. 12, p. 1110-1118en_HK
dc.identifier.issn0305-1870en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91552-
dc.description.abstract1. The aim of the present study was to determine the temporal changes in tissue adrenomedullin (AM) and cytokine contents and cytokine and preproAM mRNA levels in the kidney, liver, adrenal gland and spleen of lipopolysaccharide (LPS)-treated rats. 2. Rats were injected with LPS (10 mg/kg, i.p.). Radioimmunoassay and solution hybridization-RNase protection assays were used to follow the changes in AM and its mRNA levels, respectively; ELISA and reverse transcription-polymerase chain reaction were used to follow the changes in cytokines and their mRNA levels, respectively. 3. In the kidney, the preproAM mRNA levels were increased 1 and 3 h after LPS treatment, whereas AM levels were decreased at 3 h. Interleukin (IL)-6 and IL-1β levels were increased at 3 and 6 h, respectively. 4. The preproAM mRNA levels were elevated in the liver 3 h after LPS injection. Concentrations of tumour necrosis factor (TNF)-α and IL-1β were increased at l and 6 h, respectively. 5. There were no changes in the levels of either preproAM mRNA or AM in the adrenal gland and the spleen. In the spleen, TNF-α levels were elevated at 1 and 3 h after LPS injection and IL-1β was elevated at 1 and 6 h after LPS injection, whereas in the adrenal gland IL-1β was elevated at 6 h after injection. 6. The mRNA levels of the three cytokines were elevated at all three time intervals examined in the kidney, liver, adrenal gland and spleen, with the exception that TNF-α mRNA was not elevated in the adrenal gland at 6 h after LPS injection and IL-1β mRNA was not elevated in the spleen at 3 and 6 h. 7. The plasma concentrations of TNF-α were increased at 1 and 3 h after LPS injection, whereas plasma concentration of IL-1β and IL-6 were elevated at 3 and 6 h for both. 8. The present results suggest that the biosynthesis and secretion of AM may be differentially regulated in various tissues of rats injected with LPS and that AM may interact with cytokines during inflammation. © 2005 Blackwell Publishing Asia Pty Ltd.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CEPen_HK
dc.relation.ispartofClinical and Experimental Pharmacology and Physiologyen_HK
dc.subjectAdrenalen_HK
dc.subjectCytokines-
dc.subjectEndotoxin-
dc.subjectKidney-
dc.subjectLiver-
dc.subjectSpleen-
dc.subject.meshAdrenal Glands - drug effects - metabolismen_HK
dc.subject.meshAdrenomedullinen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshInterleukins - biosynthesis - blooden_HK
dc.subject.meshKidney - drug effects - metabolismen_HK
dc.subject.meshLipopolysaccharides - pharmacologyen_HK
dc.subject.meshLiver - drug effects - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshNuclease Protection Assaysen_HK
dc.subject.meshPeptides - blood - metabolismen_HK
dc.subject.meshRNA - analysis - biosynthesisen_HK
dc.subject.meshRNA, Messenger - analysis - biosynthesisen_HK
dc.subject.meshRadioimmunoassayen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshRegional Blood Flow - drug effectsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshSpleen - drug effects - metabolismen_HK
dc.subject.meshTissue Distributionen_HK
dc.subject.meshTumor Necrosis Factor-alpha - biosynthesisen_HK
dc.titleDifferential induction of adrenomedullin, interleukins and tumour necrosis factor-α by lipopolysaccharide in rat tissues in vivoen_HK
dc.typeArticleen_HK
dc.identifier.emailCheung, BM:mycheung@hku.hken_HK
dc.identifier.emailTang, F:ftang@hkucc.hku.hken_HK
dc.identifier.authorityCheung, BM=rp01321en_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1440-1681.2005.04307.xen_HK
dc.identifier.pmid16445578-
dc.identifier.scopuseid_2-s2.0-33644975207en_HK
dc.identifier.hkuros123603-
dc.identifier.hkuros117622-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33644975207&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume32en_HK
dc.identifier.issue12en_HK
dc.identifier.spage1110en_HK
dc.identifier.epage1118en_HK
dc.identifier.eissn1440-1681-
dc.identifier.isiWOS:000234715900014-
dc.publisher.placeAustraliaen_HK
dc.identifier.scopusauthoridLi, YY=8293496300en_HK
dc.identifier.scopusauthoridWong, LY=24476809800en_HK
dc.identifier.scopusauthoridCheung, BM=7103294806en_HK
dc.identifier.scopusauthoridHwang, IS=7201615103en_HK
dc.identifier.scopusauthoridTang, F=7201979770en_HK
dc.identifier.citeulike479744-

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