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Article: Effects of iron oxide nanoparticles on cardiac differentiation of embryonic stem cells

TitleEffects of iron oxide nanoparticles on cardiac differentiation of embryonic stem cells
Authors
KeywordsCardiac differentiation
Embryonic stem cell
Superparamagnetic iron oxide
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2009, v. 379 n. 4, p. 898-903 How to Cite?
AbstractThe therapeutic potential of transplantation of embryonic stem cells (ESCs) in animal model of myocardial infarction has been consistently demonstrated. The development of superparamagnetic iron oxide (SPIO) nanoparticles labeling and cardiac magnetic resonance imaging (MRI) have been increasingly used to track the migration of transplanted cells in vivo allowing cell fate determination. However, the impact of SPIO- labeling on cell phenotype and cardiac differentiation capacity of ESCs remains unclear. In this study, we demonstrated that ESCs labeled with SPIO compared to their unlabeled counterparts had similar cardiogenic capacity, and SPIO-labeling did not affect calcium-handling property of ESC-derived cardiomyocytes. Moreover, transplantation of SPIO-labeled ESCs via direct intra-myocardial injection to infarct myocardium resulted in significant improvement in heart function. These findings demonstrated the feasibility of in vivo ESC tracking using SPIO-labeling and cardiac MRI without affecting the cardiac differentiation potential and functional properties of ESCs. © 2009 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/91551
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of Hong Kong, General Research FundHKU 7594/05M
Funding Information:

This Study was supported by Research Grants Council of Hong Kong, General Research Fund (No. HKU 7594/05M),

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorAu, KWen_HK
dc.contributor.authorLiao, SYen_HK
dc.contributor.authorLee, YKen_HK
dc.contributor.authorLai, WHen_HK
dc.contributor.authorNg, KMen_HK
dc.contributor.authorChan, YCen_HK
dc.contributor.authorYip, MCen_HK
dc.contributor.authorHo, CYen_HK
dc.contributor.authorWu, EXen_HK
dc.contributor.authorLi, RAen_HK
dc.contributor.authorSiu, CWen_HK
dc.contributor.authorTse, HFen_HK
dc.date.accessioned2010-09-17T10:21:14Z-
dc.date.available2010-09-17T10:21:14Z-
dc.date.issued2009en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2009, v. 379 n. 4, p. 898-903en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/91551-
dc.description.abstractThe therapeutic potential of transplantation of embryonic stem cells (ESCs) in animal model of myocardial infarction has been consistently demonstrated. The development of superparamagnetic iron oxide (SPIO) nanoparticles labeling and cardiac magnetic resonance imaging (MRI) have been increasingly used to track the migration of transplanted cells in vivo allowing cell fate determination. However, the impact of SPIO- labeling on cell phenotype and cardiac differentiation capacity of ESCs remains unclear. In this study, we demonstrated that ESCs labeled with SPIO compared to their unlabeled counterparts had similar cardiogenic capacity, and SPIO-labeling did not affect calcium-handling property of ESC-derived cardiomyocytes. Moreover, transplantation of SPIO-labeled ESCs via direct intra-myocardial injection to infarct myocardium resulted in significant improvement in heart function. These findings demonstrated the feasibility of in vivo ESC tracking using SPIO-labeling and cardiac MRI without affecting the cardiac differentiation potential and functional properties of ESCs. © 2009 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectCardiac differentiationen_HK
dc.subjectEmbryonic stem cellen_HK
dc.subjectSuperparamagnetic iron oxideen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Differentiation - drug effectsen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshCell Movement - drug effectsen_HK
dc.subject.meshEmbryonic Stem Cells - cytology - drug effects - transplantationen_HK
dc.subject.meshFerric Compounds - adverse effectsen_HK
dc.subject.meshGreen Fluorescent Proteins - analysis - biosynthesisen_HK
dc.subject.meshMagnetic Resonance Imaging - methodsen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMyocytes, Cardiac - cytology - drug effectsen_HK
dc.subject.meshNanoparticles - adverse effectsen_HK
dc.subject.meshStaining and Labeling - methodsen_HK
dc.titleEffects of iron oxide nanoparticles on cardiac differentiation of embryonic stem cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, YC:yauchi@graduate.hku.hken_HK
dc.identifier.emailWu, EX:ewu1@hkucc.hku.hken_HK
dc.identifier.emailLi, RA:ronaldli@hkucc.hku.hken_HK
dc.identifier.emailSiu, CW:cwdsiu@hkucc.hku.hken_HK
dc.identifier.emailTse, HF:hftse@hkucc.hku.hken_HK
dc.identifier.authorityChan, YC=rp01502en_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.identifier.authorityLi, RA=rp01352en_HK
dc.identifier.authoritySiu, CW=rp00534en_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.bbrc.2008.12.160en_HK
dc.identifier.pmid19135029-
dc.identifier.scopuseid_2-s2.0-59249094356en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-59249094356&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume379en_HK
dc.identifier.issue4en_HK
dc.identifier.spage898en_HK
dc.identifier.epage903en_HK
dc.identifier.eissn1090-2104-
dc.identifier.isiWOS:000263336700019-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectEmbryonic stem cell transplantation as a novel therapy for post-infarct left ventricular remodeling-
dc.identifier.scopusauthoridAu, KW=9738204200en_HK
dc.identifier.scopusauthoridLiao, SY=22433820700en_HK
dc.identifier.scopusauthoridLee, YK=25958641200en_HK
dc.identifier.scopusauthoridLai, WH=18434390500en_HK
dc.identifier.scopusauthoridNg, KM=7403178872en_HK
dc.identifier.scopusauthoridChan, YC=7403676116en_HK
dc.identifier.scopusauthoridYip, MC=36741863200en_HK
dc.identifier.scopusauthoridHo, CY=25958716700en_HK
dc.identifier.scopusauthoridWu, EX=7202128034en_HK
dc.identifier.scopusauthoridLi, RA=7404724466en_HK
dc.identifier.scopusauthoridSiu, CW=7006550690en_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK

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