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Article: Label-free separation of human embryonic stem cells and their cardiac derivatives using Raman spectroscopy

TitleLabel-free separation of human embryonic stem cells and their cardiac derivatives using Raman spectroscopy
Authors
KeywordsReferences (37) View In Table Layout
Issue Date2009
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ac
Citation
Analytical Chemistry, 2009, v. 81 n. 4, p. 1324-1331 How to Cite?
AbstractSelf-renewable, pluripotent human embryonic stem cells (hESCs) can be differentiated into cardiomyocytes (CMs), providing an unlimited source of cells for transplantation therapies. However, unlike certain cell lineages such as hematopoietic cells, CMs lack specific surface markers for convenient identification, physical separation, and enrichment. Identification by immunostaining of cardiac-specific proteins such as troponin requires permeabili-zation, which renders the cells unviable and nonrecover-able. Ectopic expression of a reporter protein under the transcriptional control of a heart-specific promoter for identifying hESC-derived CMs (hESC-CMs) is useful for research but complicates potential clinical applications. The practical detection and removal of undifferentiated hESCs in a graft, which may lead to tumors, is also critical. Here, we demonstrate a nondestructive, label-free optical method based on Raman scattering to interrogate the intrinsic biochemical signatures of individual hESCs and their cardiac derivatives, allowing cells to be identified and classified. By combination of the Raman spectroscopic data with multivariate statistical analysis, our results indicate that hESCs, human fetal left ventricular CMs, and hESC-CMs can be identified by their intrinsic biochemical characteristics with an accuracy of 96%, 98%, and 66%, respectively. The present study lays the groundwork for developing a systematic and automated method for the noninvasive and label-free sorting of (i) high-quality hESCs for expansion and (ii) ex vivo CMs (derived from embryonic or adult stem cells) for cell-based heart therapies. © 2009 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/91542
ISSN
2015 Impact Factor: 5.886
2015 SCImago Journal Rankings: 2.369
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, JWen_HK
dc.contributor.authorLieu, DKen_HK
dc.contributor.authorHuser, Ten_HK
dc.contributor.authorLi, RAen_HK
dc.date.accessioned2010-09-17T10:21:05Z-
dc.date.available2010-09-17T10:21:05Z-
dc.date.issued2009en_HK
dc.identifier.citationAnalytical Chemistry, 2009, v. 81 n. 4, p. 1324-1331en_HK
dc.identifier.issn0003-2700en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91542-
dc.description.abstractSelf-renewable, pluripotent human embryonic stem cells (hESCs) can be differentiated into cardiomyocytes (CMs), providing an unlimited source of cells for transplantation therapies. However, unlike certain cell lineages such as hematopoietic cells, CMs lack specific surface markers for convenient identification, physical separation, and enrichment. Identification by immunostaining of cardiac-specific proteins such as troponin requires permeabili-zation, which renders the cells unviable and nonrecover-able. Ectopic expression of a reporter protein under the transcriptional control of a heart-specific promoter for identifying hESC-derived CMs (hESC-CMs) is useful for research but complicates potential clinical applications. The practical detection and removal of undifferentiated hESCs in a graft, which may lead to tumors, is also critical. Here, we demonstrate a nondestructive, label-free optical method based on Raman scattering to interrogate the intrinsic biochemical signatures of individual hESCs and their cardiac derivatives, allowing cells to be identified and classified. By combination of the Raman spectroscopic data with multivariate statistical analysis, our results indicate that hESCs, human fetal left ventricular CMs, and hESC-CMs can be identified by their intrinsic biochemical characteristics with an accuracy of 96%, 98%, and 66%, respectively. The present study lays the groundwork for developing a systematic and automated method for the noninvasive and label-free sorting of (i) high-quality hESCs for expansion and (ii) ex vivo CMs (derived from embryonic or adult stem cells) for cell-based heart therapies. © 2009 American Chemical Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/acen_HK
dc.relation.ispartofAnalytical Chemistryen_HK
dc.subjectReferences (37) View In Table Layouten_HK
dc.subject.meshAdult Stem Cells - cytology-
dc.subject.meshCell Line-
dc.subject.meshCell Separation - methods-
dc.subject.meshEmbryonic Stem Cells - cytology-
dc.subject.meshMyocytes, Cardiac - cytology-
dc.titleLabel-free separation of human embryonic stem cells and their cardiac derivatives using Raman spectroscopyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-2700&volume=81&issue=4&spage=1324&epage=1331&date=2009&atitle=Label-free+separation+of+human+embryonic+stem+cells+and+their+cardiac+derivatives+using+raman+spectroscopy-
dc.identifier.emailLi, RA:ronaldli@hkucc.hku.hken_HK
dc.identifier.authorityLi, RA=rp01352en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1021/ac801665men_HK
dc.identifier.pmid19152312en_HK
dc.identifier.pmcidPMC2652839-
dc.identifier.scopuseid_2-s2.0-63649089721en_HK
dc.identifier.hkuros182846-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-63649089721&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume81en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1324en_HK
dc.identifier.epage1331en_HK
dc.identifier.isiWOS:000263319000004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, JW=26432534400en_HK
dc.identifier.scopusauthoridLieu, DK=7003924538en_HK
dc.identifier.scopusauthoridHuser, T=7004227222en_HK
dc.identifier.scopusauthoridLi, RA=7404724466en_HK

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