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Article: Electrophysiological properties of pluripotent human and mouse embryonic stem cells

TitleElectrophysiological properties of pluripotent human and mouse embryonic stem cells
Authors
KeywordsSpecies Index: Animalia
Issue Date2005
PublisherAlphaMed Press, Inc. The Journal's web site is located at http://www.stemcells.com
Citation
Stem Cells, 2005, v. 23 n. 10, p. 1526-1534 How to Cite?
AbstractPluripotent embryonic stem cells (ESCs) possess promising potential for cell-based therapies, but their electrophysiological properties have not been characterized. Here we describe the presence of ionic currents in mouse (m) and human (h) ESCs and their physiological function. In mESCs, tetraethylammonium (TEA)-sensitive depolarization-activated delayed rectifier K + currents (IK DR) (8.6 ± 0.9 pA/pF at +40 mV; IC 50 = 1.2 ± 0.3 mM), which contained components sensitive to 4-aminopyridine (4-AP) (IC 50 = 0.5 ± 0.1 mM) and 100 nM Ca 2+- activated K + current (IK Ca) blocker iberiotoxin (IBTX), were detected in 52.3% of undifferentiated cells. IK DR was similarly present in hESCs (∼100%) but with an approximately sixfold higher current density (47.5 ± 7.9 pA/pF at +40 mV). When assayed by bromodeoxyurindine incorporation, application of TEA, 4-AP, or IBTX significantly reduced the proliferation of mESCs and hESCs in a dose-dependent manner (p < .05). A hyperpolarization-activated inward current (I h) (-2.2 ± 0.4 pA/pF at -120 mV) was detected in 23% of mESCs but not hESCs. Neither Na v nor Ca v currents were detected in mESCs and hESCs. Microarray and reverse transcription-polymerase chain reaction analyses identified several candidate genes for the ionic currents discovered. Collectively, our results indicate that pluripotent ESCs functionally express several specialized ion channels and further highlight similarities and differences between the two species. Practical considerations for the therapeutic use of ESCs are discussed.
Persistent Identifierhttp://hdl.handle.net/10722/91531
ISSN
2015 Impact Factor: 5.902
2015 SCImago Journal Rankings: 3.438
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Ken_HK
dc.contributor.authorXue, Ten_HK
dc.contributor.authorTsang, SYen_HK
dc.contributor.authorVan Huizen, Ren_HK
dc.contributor.authorWong, CWen_HK
dc.contributor.authorLai, KWen_HK
dc.contributor.authorYe, Zen_HK
dc.contributor.authorCheng, Len_HK
dc.contributor.authorAu, KWen_HK
dc.contributor.authorZhang, Jen_HK
dc.contributor.authorLi, GRen_HK
dc.contributor.authorLau, CPen_HK
dc.contributor.authorTse, HFen_HK
dc.contributor.authorLi, RAen_HK
dc.date.accessioned2010-09-17T10:20:54Z-
dc.date.available2010-09-17T10:20:54Z-
dc.date.issued2005en_HK
dc.identifier.citationStem Cells, 2005, v. 23 n. 10, p. 1526-1534en_HK
dc.identifier.issn1066-5099en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91531-
dc.description.abstractPluripotent embryonic stem cells (ESCs) possess promising potential for cell-based therapies, but their electrophysiological properties have not been characterized. Here we describe the presence of ionic currents in mouse (m) and human (h) ESCs and their physiological function. In mESCs, tetraethylammonium (TEA)-sensitive depolarization-activated delayed rectifier K + currents (IK DR) (8.6 ± 0.9 pA/pF at +40 mV; IC 50 = 1.2 ± 0.3 mM), which contained components sensitive to 4-aminopyridine (4-AP) (IC 50 = 0.5 ± 0.1 mM) and 100 nM Ca 2+- activated K + current (IK Ca) blocker iberiotoxin (IBTX), were detected in 52.3% of undifferentiated cells. IK DR was similarly present in hESCs (∼100%) but with an approximately sixfold higher current density (47.5 ± 7.9 pA/pF at +40 mV). When assayed by bromodeoxyurindine incorporation, application of TEA, 4-AP, or IBTX significantly reduced the proliferation of mESCs and hESCs in a dose-dependent manner (p < .05). A hyperpolarization-activated inward current (I h) (-2.2 ± 0.4 pA/pF at -120 mV) was detected in 23% of mESCs but not hESCs. Neither Na v nor Ca v currents were detected in mESCs and hESCs. Microarray and reverse transcription-polymerase chain reaction analyses identified several candidate genes for the ionic currents discovered. Collectively, our results indicate that pluripotent ESCs functionally express several specialized ion channels and further highlight similarities and differences between the two species. Practical considerations for the therapeutic use of ESCs are discussed.en_HK
dc.languageengen_HK
dc.publisherAlphaMed Press, Inc. The Journal's web site is located at http://www.stemcells.comen_HK
dc.relation.ispartofStem Cellsen_HK
dc.subjectSpecies Index: Animaliaen_HK
dc.subject.mesh4-Aminopyridine - pharmacologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshElectrophysiologyen_HK
dc.subject.meshEmbryo Researchen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMicroarray Analysisen_HK
dc.subject.meshPeptides - pharmacologyen_HK
dc.subject.meshPluripotent Stem Cells - cytology - physiologyen_HK
dc.subject.meshPotassium Channel Blockers - pharmacologyen_HK
dc.subject.meshPotassium Channels - drug effects - physiologyen_HK
dc.subject.meshSpecies Specificityen_HK
dc.subject.meshTetraethylammonium - pharmacologyen_HK
dc.titleElectrophysiological properties of pluripotent human and mouse embryonic stem cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailLi, GR:grli@hkucc.hku.hken_HK
dc.identifier.emailTse, HF:hftse@hkucc.hku.hken_HK
dc.identifier.emailLi, RA:ronaldli@hkucc.hku.hken_HK
dc.identifier.authorityLi, GR=rp00476en_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.identifier.authorityLi, RA=rp01352en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1634/stemcells.2004-0299en_HK
dc.identifier.pmid16091557en_HK
dc.identifier.scopuseid_2-s2.0-28444449787en_HK
dc.identifier.hkuros112871-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-28444449787&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1526en_HK
dc.identifier.epage1534en_HK
dc.identifier.isiWOS:000233708700011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, K=35286098800en_HK
dc.identifier.scopusauthoridXue, T=7005064190en_HK
dc.identifier.scopusauthoridTsang, SY=7102255908en_HK
dc.identifier.scopusauthoridVan Huizen, R=6602947598en_HK
dc.identifier.scopusauthoridWong, CW=9736145600en_HK
dc.identifier.scopusauthoridLai, KW=7402135541en_HK
dc.identifier.scopusauthoridYe, Z=7401957009en_HK
dc.identifier.scopusauthoridCheng, L=7403337362en_HK
dc.identifier.scopusauthoridAu, KW=9738204200en_HK
dc.identifier.scopusauthoridZhang, J=7601353887en_HK
dc.identifier.scopusauthoridLi, GR=7408462932en_HK
dc.identifier.scopusauthoridLau, CP=7401968501en_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK
dc.identifier.scopusauthoridLi, RA=7404724466en_HK
dc.identifier.citeulike2623997-

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