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Article: Transcriptional and functional profilling of human embryonic stem cell-derived cardiomyocytes

TitleTranscriptional and functional profilling of human embryonic stem cell-derived cardiomyocytes
Authors
Issue Date2008
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2008, v. 3 n. 10 How to Cite?
AbstractHuman embryonic stemcells (hESCs) can serve as a potentially limitless source of cells that may enable regeneration of diseased tissue and organs. Here we investigate the use of human embryonic stemcell-derived cardiomyocytes (hESC-CMs) in promoting recovery from cardiac ischemia reperfusion injury in a mouse model. Using microarrays, we have described the hESC-CM transcriptome within the spectrum of changes that occur between undifferentiated hESCs and fetal heart cells. The hESC-CMs expressed cardiomyocyte genes at levels similar to those found in 20-week fetal heart cells, making this population a good source of potential replacement cells in vivo. Echocardiographic studies showed significant improvement in heart function by 8 weeks after transplantation. Finally, we demonstrate long-term engraftment of hESC-CMs by using molecular imaging to track cellular localization, survival, and proliferation in vivo. Taken together, global gene expression profiling of hESC differentiation enables a systems-based analysis of the biological processes, networks, and genes that drive hESC fate decisions, and studies such as this will serve as the foundation for future clinical applications of stem cell therapies. © 2008 Cao et al.
Persistent Identifierhttp://hdl.handle.net/10722/91518
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
BWF CAMS
Stanford Cardiovascular Institute
NIHHL089027
CA114747
HL076445
HL72857
CIRMRS1-00322
Funding Information:

This work was supported by grants from the BWF CAMS, Stanford Cardiovascular Institute, NIH HL089027, and CIRM RS1-00322 (JCW). This work was also supported in part by NIH CA114747 (SSG), NIH HL076445 (RAW), and NIH HL72857 (RAL).

References

 

DC FieldValueLanguage
dc.contributor.authorCao, Fen_HK
dc.contributor.authorWagner, RAen_HK
dc.contributor.authorWilson, KDen_HK
dc.contributor.authorXie, Xen_HK
dc.contributor.authorFu, JDen_HK
dc.contributor.authorDrukker, Men_HK
dc.contributor.authorLee, Aen_HK
dc.contributor.authorLi, RAen_HK
dc.contributor.authorGambhir, SSen_HK
dc.contributor.authorWeissman, ILen_HK
dc.contributor.authorRobbins, RCen_HK
dc.contributor.authorWu, JCen_HK
dc.date.accessioned2010-09-17T10:20:42Z-
dc.date.available2010-09-17T10:20:42Z-
dc.date.issued2008en_HK
dc.identifier.citationPlos One, 2008, v. 3 n. 10en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91518-
dc.description.abstractHuman embryonic stemcells (hESCs) can serve as a potentially limitless source of cells that may enable regeneration of diseased tissue and organs. Here we investigate the use of human embryonic stemcell-derived cardiomyocytes (hESC-CMs) in promoting recovery from cardiac ischemia reperfusion injury in a mouse model. Using microarrays, we have described the hESC-CM transcriptome within the spectrum of changes that occur between undifferentiated hESCs and fetal heart cells. The hESC-CMs expressed cardiomyocyte genes at levels similar to those found in 20-week fetal heart cells, making this population a good source of potential replacement cells in vivo. Echocardiographic studies showed significant improvement in heart function by 8 weeks after transplantation. Finally, we demonstrate long-term engraftment of hESC-CMs by using molecular imaging to track cellular localization, survival, and proliferation in vivo. Taken together, global gene expression profiling of hESC differentiation enables a systems-based analysis of the biological processes, networks, and genes that drive hESC fate decisions, and studies such as this will serve as the foundation for future clinical applications of stem cell therapies. © 2008 Cao et al.en_HK
dc.languageengen_HK
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPLoS ONEen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshEmbryonic Stem Cells - cytology - transplantation-
dc.subject.meshGene Expression Profiling-
dc.subject.meshMyocardial Reperfusion Injury - therapy-
dc.subject.meshMyocytes, Cardiac - cytology - transplantation-
dc.subject.meshStem Cell Transplantation - methods-
dc.titleTranscriptional and functional profilling of human embryonic stem cell-derived cardiomyocytesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1932-6203&volume=3&issue=10&spage=e3474&epage=&date=2008&atitle=Transcriptional+and+Functional+Profiling+of+Human+Embryonic+Stem+Cell-Derived+Cardiomyocytes-
dc.identifier.emailLi, RA:ronaldli@hkucc.hku.hken_HK
dc.identifier.authorityLi, RA=rp01352en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0003474en_HK
dc.identifier.pmid18941512-
dc.identifier.pmcidPMC2565131-
dc.identifier.scopuseid_2-s2.0-55649098531en_HK
dc.identifier.hkuros182849-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-55649098531&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume3en_HK
dc.identifier.issue10en_HK
dc.identifier.spagee3474-
dc.identifier.epagee3474-
dc.identifier.isiWOS:000265126100005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCao, F=35072497500en_HK
dc.identifier.scopusauthoridWagner, RA=7404292272en_HK
dc.identifier.scopusauthoridWilson, KD=24726465500en_HK
dc.identifier.scopusauthoridXie, X=12786458300en_HK
dc.identifier.scopusauthoridFu, JD=7401722481en_HK
dc.identifier.scopusauthoridDrukker, M=12787035400en_HK
dc.identifier.scopusauthoridLee, A=35790108100en_HK
dc.identifier.scopusauthoridLi, RA=7404724466en_HK
dc.identifier.scopusauthoridGambhir, SS=7101904349en_HK
dc.identifier.scopusauthoridWeissman, IL=36038861200en_HK
dc.identifier.scopusauthoridRobbins, RC=7202460248en_HK
dc.identifier.scopusauthoridWu, JC=7409253259en_HK

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