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Article: Critical intra-linker interactions of HCN1-encoded pacemaker channels revealed by interchange of S3-S4 determinants

TitleCritical intra-linker interactions of HCN1-encoded pacemaker channels revealed by interchange of S3-S4 determinants
Authors
KeywordsActivation
HCN
Interchange
S3-S4 linker
Issue Date2004
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2004, v. 322 n. 2, p. 652-658 How to Cite?
AbstractHyperpolarization-activated cyclic nucleotide-modulated (HCN) channels contribute to the spontaneous rhythmic activities in cardiac and neuronal cells. Recently, we reported that the S3-S4 linker of HCN1 channels influences activation, and that part of the linker is helical with the determinants G231, M232, and E235 clustered on one side. Here we explored the undefined role of the G 231E 235M 232 triplet by systematic substitutions. Replacing G231 or M232 next to the "neighboring" E235 in the S3-S4 helix with an anionic residue (i.e., G231E, M232E) rendered channels non-functional although they were localized on the membrane surface. Interestingly, this loss of function could be readily rescued either by introducing a countercharge at position 235 (G231E/E235R, M232E/E235R) or by interchanging residues 231 or 232 and 235 (G231E/E235G, M232E/E235M). We conclude that residues 231, 232, and 235 are in close spatial proximity to each other, and uniquely interact with one another to shape the phenotypes of HCN channels. © 2004 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/91497
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTsang, SYen_HK
dc.contributor.authorLesso, Hen_HK
dc.contributor.authorLi, RAen_HK
dc.date.accessioned2010-09-17T10:20:22Z-
dc.date.available2010-09-17T10:20:22Z-
dc.date.issued2004en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2004, v. 322 n. 2, p. 652-658en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/91497-
dc.description.abstractHyperpolarization-activated cyclic nucleotide-modulated (HCN) channels contribute to the spontaneous rhythmic activities in cardiac and neuronal cells. Recently, we reported that the S3-S4 linker of HCN1 channels influences activation, and that part of the linker is helical with the determinants G231, M232, and E235 clustered on one side. Here we explored the undefined role of the G 231E 235M 232 triplet by systematic substitutions. Replacing G231 or M232 next to the "neighboring" E235 in the S3-S4 helix with an anionic residue (i.e., G231E, M232E) rendered channels non-functional although they were localized on the membrane surface. Interestingly, this loss of function could be readily rescued either by introducing a countercharge at position 235 (G231E/E235R, M232E/E235R) or by interchanging residues 231 or 232 and 235 (G231E/E235G, M232E/E235M). We conclude that residues 231, 232, and 235 are in close spatial proximity to each other, and uniquely interact with one another to shape the phenotypes of HCN channels. © 2004 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectActivationen_HK
dc.subjectHCNen_HK
dc.subjectInterchangeen_HK
dc.subjectS3-S4 linkeren_HK
dc.titleCritical intra-linker interactions of HCN1-encoded pacemaker channels revealed by interchange of S3-S4 determinantsen_HK
dc.typeArticleen_HK
dc.identifier.emailLi, RA:ronaldli@hkucc.hku.hken_HK
dc.identifier.authorityLi, RA=rp01352en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbrc.2004.07.167en_HK
dc.identifier.scopuseid_2-s2.0-6044244939en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-6044244939&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume322en_HK
dc.identifier.issue2en_HK
dc.identifier.spage652en_HK
dc.identifier.epage658en_HK
dc.identifier.isiWOS:000223675800042-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTsang, SY=7102255908en_HK
dc.identifier.scopusauthoridLesso, H=6507506626en_HK
dc.identifier.scopusauthoridLi, RA=7404724466en_HK

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