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Article: Establishment and expression profiling of new lung cancer cell lines from Chinese smokers and lifetime never-smokers

TitleEstablishment and expression profiling of new lung cancer cell lines from Chinese smokers and lifetime never-smokers
Authors
KeywordsCell line
Lung adenocarcinoma
Non-smokers
Issue Date2006
PublisherLippincott Williams & Wilkins
Citation
Journal of Thoracic Oncology, 2006, v. 1 n. 9, p. 932-942 How to Cite?
AbstractBACKGROUND: Bronchogenic adenocarcinoma is the predominant histologic subtype of lung cancer, which ranks top in the cancer mortality in both men and women. Female nonsmokers and adenocarcinoma have emerged as a distinct combination in patients with lung cancer in recent decades. Lung cancer cell lines established from patients with known clinical characteristics such as gender and smoking habit would be useful for future research on lung cancer. METHODS: Four new lung adenocarcinoma cell lines (HKULC 1-4) were established from Chinese patients with primary lung adenocarcinomas and with different clinical characteristics with respect to age, gender, smoking habits, tumor staging, and previous therapy. They were characterized by immunohistochemical and growth kinetic studies, tests for tumorigenicity in nude mice, epidermal growth factor receptor (EGFR) gene mutation analysis, and in situ hybridization, and gene expression profiling with Affymetrix GeneChip HG-U133A. RESULTS: The newly established HKULC lung adenocarcinoma cell lines were maintained for over 70 passages and demonstrated morphologic and immunohistochemical features and growth kinetics of tumor cell lines. One of the four HKULC cell lines, HKULC 3 (derived from a female nonsmoking patient with lung adenocarcinoma), was found to have a deletion at exon 19 of the EGFR gene. EGFR in situ hybridization showed no EGFR gene amplification in these cell lines. HKULC 1 and 4 formed tumor xenografts after inoculation in nude mice. A list of 71 genes that were differentially expressed or showing class predictive significance was identified. These genes included putative tumor suppressor genes (DKK3, SERPINF1, CDH11, DSC3, and KLF6), genes involved in or related to the EGFR pathways (ERBB3, MUC1, VAV1), genes involved in regulation of cell cycle and proliferation (CDKN1A and CDKN2A), a putative oncogene (EEF1A2), and a gene related to metastasis (MTSS1). DISCUSSION: Four new lung adenocarcinoma cell lines were established from patients with different clinical characteristics. These characterized cell lines and their gene expression profiles will provide resources for studies of lung cancer biology and in vitro chemotherapeutic drug study. © 2006International Association for the Study of Lung Cancer.
Persistent Identifierhttp://hdl.handle.net/10722/91447
ISSN
2015 Impact Factor: 5.04
2015 SCImago Journal Rankings: 2.597
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLam, DCLen_HK
dc.contributor.authorGirard, Len_HK
dc.contributor.authorSuen, WSen_HK
dc.contributor.authorChung, LPen_HK
dc.contributor.authorTin, VPCen_HK
dc.contributor.authorLam, WKen_HK
dc.contributor.authorMinna, JDen_HK
dc.contributor.authorWong, MPen_HK
dc.date.accessioned2010-09-17T10:19:34Z-
dc.date.available2010-09-17T10:19:34Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal of Thoracic Oncology, 2006, v. 1 n. 9, p. 932-942en_HK
dc.identifier.issn1556-0864en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91447-
dc.description.abstractBACKGROUND: Bronchogenic adenocarcinoma is the predominant histologic subtype of lung cancer, which ranks top in the cancer mortality in both men and women. Female nonsmokers and adenocarcinoma have emerged as a distinct combination in patients with lung cancer in recent decades. Lung cancer cell lines established from patients with known clinical characteristics such as gender and smoking habit would be useful for future research on lung cancer. METHODS: Four new lung adenocarcinoma cell lines (HKULC 1-4) were established from Chinese patients with primary lung adenocarcinomas and with different clinical characteristics with respect to age, gender, smoking habits, tumor staging, and previous therapy. They were characterized by immunohistochemical and growth kinetic studies, tests for tumorigenicity in nude mice, epidermal growth factor receptor (EGFR) gene mutation analysis, and in situ hybridization, and gene expression profiling with Affymetrix GeneChip HG-U133A. RESULTS: The newly established HKULC lung adenocarcinoma cell lines were maintained for over 70 passages and demonstrated morphologic and immunohistochemical features and growth kinetics of tumor cell lines. One of the four HKULC cell lines, HKULC 3 (derived from a female nonsmoking patient with lung adenocarcinoma), was found to have a deletion at exon 19 of the EGFR gene. EGFR in situ hybridization showed no EGFR gene amplification in these cell lines. HKULC 1 and 4 formed tumor xenografts after inoculation in nude mice. A list of 71 genes that were differentially expressed or showing class predictive significance was identified. These genes included putative tumor suppressor genes (DKK3, SERPINF1, CDH11, DSC3, and KLF6), genes involved in or related to the EGFR pathways (ERBB3, MUC1, VAV1), genes involved in regulation of cell cycle and proliferation (CDKN1A and CDKN2A), a putative oncogene (EEF1A2), and a gene related to metastasis (MTSS1). DISCUSSION: Four new lung adenocarcinoma cell lines were established from patients with different clinical characteristics. These characterized cell lines and their gene expression profiles will provide resources for studies of lung cancer biology and in vitro chemotherapeutic drug study. © 2006International Association for the Study of Lung Cancer.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkinsen_HK
dc.relation.ispartofJournal of Thoracic Oncologyen_HK
dc.rightsJournal of Thoracic Oncology. Copyright © Lippincott Williams & Wilkins.-
dc.subjectCell lineen_HK
dc.subjectLung adenocarcinomaen_HK
dc.subjectNon-smokersen_HK
dc.titleEstablishment and expression profiling of new lung cancer cell lines from Chinese smokers and lifetime never-smokersen_HK
dc.typeArticleen_HK
dc.identifier.emailLam, DCL: dcllam@hku.hken_HK
dc.identifier.emailChung, LP: lpchung@hkucc.hku.hken_HK
dc.identifier.emailTin, PC: vickytin@pathology.hku.hken_HK
dc.identifier.emailLam, WK: lamwk@hku.hk-
dc.identifier.emailWong, MP: mwpik@hkucc.hku.hk-
dc.identifier.authorityLam, DCL=rp01345en_HK
dc.identifier.authorityChung, LP=rp00249en_HK
dc.identifier.authorityWong, MP=rp00348en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1097/01243894-200611000-00003en_HK
dc.identifier.scopuseid_2-s2.0-34247850957en_HK
dc.identifier.hkuros135047-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34247850957&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1en_HK
dc.identifier.issue9en_HK
dc.identifier.spage932en_HK
dc.identifier.epage942en_HK
dc.identifier.eissn1556-1380-
dc.identifier.isiWOS:000242248800003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLam, DCL=7201749615en_HK
dc.identifier.scopusauthoridGirard, L=7101715512en_HK
dc.identifier.scopusauthoridSuen, WS=12786607600en_HK
dc.identifier.scopusauthoridChung, LP=24315879100en_HK
dc.identifier.scopusauthoridTin, VPC=6603199735en_HK
dc.identifier.scopusauthoridLam, WK=7203021937en_HK
dc.identifier.scopusauthoridMinna, JD=35380041500en_HK
dc.identifier.scopusauthoridWong, MP=7403907887en_HK
dc.customcontrol.immutablesml 130620-

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