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Article: CpG island methylator phenotype association with elevated serum α-fetoprotein level in hepatocellular carcinoma

TitleCpG island methylator phenotype association with elevated serum α-fetoprotein level in hepatocellular carcinoma
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2007
PublisherAmerican Association for Cancer Research
Citation
Clinical Cancer Research, 2007, v. 13 n. 3, p. 944-952 How to Cite?
AbstractPurpose: CpG island methylator phenotype (CIMP) involves hypermethylation targeted toward the promoters of multiple genes. To gain insight into the role of epigenetic aberration of tumor-related genes in hepatocarcinogenesis, we determined a hypermethylation profile in hepatocellular carcinoma (HCC). Experimental Design: We examined the promoter methylation status of nine genes in 50 HCCs, 50 paired nontumor tissues, and 6 normal liver tissues by methylation-specific PCR. CIMP+ was defined as having five genes that are concordantly methylated. Results: The frequency of promoter methylation of nine genes in 50 HCCs varied from 10% in P53 to 94% in c-Myc. The methylation status of P14, P15, P16, ER, RASSF1A, WT1, and c-Myc was significantly correlated with HCC and nontumor tissues (P < 0.05). Hypermethylation of one or more genes was found in 96% of HCC. CIMP was more frequent in HCC than in nontumor tissues (70% and 12%, P < 0.001). There is a significant association between CIMP and methylation of P14, P15, P16, ER, RSAAF1A, and WT1 (P < 0.05) and serum α-fetoprotein (AFP) level (P = 0.017). CIMP+ was more frequent in HCC with AFP ≥ 30 μg/L than those with AFP < 30 μg/L (P = 0.005). In addition, the promoter hypermethylation of P15 and P16 was associated with elevated serum AFP levels in 35 HCC samples with CIMP+ (P < 0.05). Conclusions: Positive correlation of CIMP and AFP levels in HCC suggests that CIMP can serve as a molecular marker of late-stage HCC development. © 2007 American Association for Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/91271
ISSN
2015 Impact Factor: 8.738
2015 SCImago Journal Rankings: 5.314
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Cen_HK
dc.contributor.authorLi, Zen_HK
dc.contributor.authorCheng, Yen_HK
dc.contributor.authorJia, Fen_HK
dc.contributor.authorLi, Ren_HK
dc.contributor.authorWu, Men_HK
dc.contributor.authorLi, Ken_HK
dc.contributor.authorWei, Len_HK
dc.date.accessioned2010-09-17T10:15:59Z-
dc.date.available2010-09-17T10:15:59Z-
dc.date.issued2007en_HK
dc.identifier.citationClinical Cancer Research, 2007, v. 13 n. 3, p. 944-952en_HK
dc.identifier.issn1078-0432en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91271-
dc.description.abstractPurpose: CpG island methylator phenotype (CIMP) involves hypermethylation targeted toward the promoters of multiple genes. To gain insight into the role of epigenetic aberration of tumor-related genes in hepatocarcinogenesis, we determined a hypermethylation profile in hepatocellular carcinoma (HCC). Experimental Design: We examined the promoter methylation status of nine genes in 50 HCCs, 50 paired nontumor tissues, and 6 normal liver tissues by methylation-specific PCR. CIMP+ was defined as having five genes that are concordantly methylated. Results: The frequency of promoter methylation of nine genes in 50 HCCs varied from 10% in P53 to 94% in c-Myc. The methylation status of P14, P15, P16, ER, RASSF1A, WT1, and c-Myc was significantly correlated with HCC and nontumor tissues (P < 0.05). Hypermethylation of one or more genes was found in 96% of HCC. CIMP was more frequent in HCC than in nontumor tissues (70% and 12%, P < 0.001). There is a significant association between CIMP and methylation of P14, P15, P16, ER, RSAAF1A, and WT1 (P < 0.05) and serum α-fetoprotein (AFP) level (P = 0.017). CIMP+ was more frequent in HCC with AFP ≥ 30 μg/L than those with AFP < 30 μg/L (P = 0.005). In addition, the promoter hypermethylation of P15 and P16 was associated with elevated serum AFP levels in 35 HCC samples with CIMP+ (P < 0.05). Conclusions: Positive correlation of CIMP and AFP levels in HCC suggests that CIMP can serve as a molecular marker of late-stage HCC development. © 2007 American Association for Cancer Research.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Researchen_HK
dc.relation.ispartofClinical Cancer Researchen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.titleCpG island methylator phenotype association with elevated serum α-fetoprotein level in hepatocellular carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.emailCheng, Y:yuecheng@hku.hken_HK
dc.identifier.authorityCheng, Y=rp1320en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1158/1078-0432.CCR-06-2268en_HK
dc.identifier.pmid17289889-
dc.identifier.scopuseid_2-s2.0-33847346367en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33847346367&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue3en_HK
dc.identifier.spage944en_HK
dc.identifier.epage952en_HK
dc.identifier.isiWOS:000244289400025-

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