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Article: Analysis of loss of heterozygosity on chromosomes 10q, 11, and 16 in medulloblastomas

TitleAnalysis of loss of heterozygosity on chromosomes 10q, 11, and 16 in medulloblastomas
Authors
KeywordsReferences (42) View In Table Layout
Issue Date2001
PublisherAmerican Association of Neurological Surgeons. The Journal's web site is located at http://www.thejns-net.org
Citation
Journal of Neurosurgery, 2001, v. 94 n. 5, p. 799-805 How to Cite?
AbstractObject. The loss of genetic material from specific chromosome loci is a common feature in the oncogenesis of tumors and is often indicative of the presence of important tumor suppressor genes at these loci. Recent molecular genetic analyses have demonstrated frequent loss of chromosomes 10q, 11, and 16 in medulloblastomas. The aim of this study was to localize the targeted deletion regions on the three aforementioned chromosomes in medulloblastomas. Methods. Loss of heterozygosity (LOH) was examined on chromosomes 10q, 11, and 16 in a series of 22 primary and two recurrent medulloblastomas by using polymerase chain reaction-based microsatellite analysis. The DNA extracted from the tumors and corresponding normal blood samples were amplified independently in the presence of radioactively labeled microsatellite primers, resolved by denaturing gel electrophoresis and processed for autoradiography. The DNA obtained from control blood samples that displayed allelic heterozygosity at a given microsatellite locus were considered informative. Loss of heterozygosity was inferred when the allelic signal intensity of the tumor sample was reduced by at least 40%, relative to that of the constitutional control. The LOH analysis demonstrated that deletions of chromosomes 10q, 11p, and 16q are recurrent genetic events in the development of medulloblastomas. Three subchromosomal regions of loss have been identified and are localized to the deleted in malignant brain tumors 1 [DMBT1] gene site on chromosomes 10q25, 11p13-11p15.1 and 16q24.1-24.3. Conclusions. These results indicate that DMBT1 is closely associated with the oncogenesis of medulloblastomas and highlight regions of loss on chromosomes 11p and 16q for further fine mapping and cloning of candidate tumor suppressor genes that are important for the genesis of medulloblastoma.
Persistent Identifierhttp://hdl.handle.net/10722/91244
ISSN
2015 Impact Factor: 3.443
2015 SCImago Journal Rankings: 1.673
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYin, X-Len_HK
dc.contributor.authorPang, JCen_HK
dc.contributor.authorLiu, Y-Hen_HK
dc.contributor.authorChong, EYen_HK
dc.contributor.authorCheng, Yen_HK
dc.contributor.authorPoon, W-Sen_HK
dc.contributor.authorNg, H-Ken_HK
dc.date.accessioned2010-09-17T10:15:32Z-
dc.date.available2010-09-17T10:15:32Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal of Neurosurgery, 2001, v. 94 n. 5, p. 799-805en_HK
dc.identifier.issn0022-3085en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91244-
dc.description.abstractObject. The loss of genetic material from specific chromosome loci is a common feature in the oncogenesis of tumors and is often indicative of the presence of important tumor suppressor genes at these loci. Recent molecular genetic analyses have demonstrated frequent loss of chromosomes 10q, 11, and 16 in medulloblastomas. The aim of this study was to localize the targeted deletion regions on the three aforementioned chromosomes in medulloblastomas. Methods. Loss of heterozygosity (LOH) was examined on chromosomes 10q, 11, and 16 in a series of 22 primary and two recurrent medulloblastomas by using polymerase chain reaction-based microsatellite analysis. The DNA extracted from the tumors and corresponding normal blood samples were amplified independently in the presence of radioactively labeled microsatellite primers, resolved by denaturing gel electrophoresis and processed for autoradiography. The DNA obtained from control blood samples that displayed allelic heterozygosity at a given microsatellite locus were considered informative. Loss of heterozygosity was inferred when the allelic signal intensity of the tumor sample was reduced by at least 40%, relative to that of the constitutional control. The LOH analysis demonstrated that deletions of chromosomes 10q, 11p, and 16q are recurrent genetic events in the development of medulloblastomas. Three subchromosomal regions of loss have been identified and are localized to the deleted in malignant brain tumors 1 [DMBT1] gene site on chromosomes 10q25, 11p13-11p15.1 and 16q24.1-24.3. Conclusions. These results indicate that DMBT1 is closely associated with the oncogenesis of medulloblastomas and highlight regions of loss on chromosomes 11p and 16q for further fine mapping and cloning of candidate tumor suppressor genes that are important for the genesis of medulloblastoma.en_HK
dc.languageengen_HK
dc.publisherAmerican Association of Neurological Surgeons. The Journal's web site is located at http://www.thejns-net.orgen_HK
dc.relation.ispartofJournal of Neurosurgeryen_HK
dc.subjectReferences (42) View In Table Layouten_HK
dc.titleAnalysis of loss of heterozygosity on chromosomes 10q, 11, and 16 in medulloblastomasen_HK
dc.typeArticleen_HK
dc.identifier.emailCheng, Y:yuecheng@hku.hken_HK
dc.identifier.authorityCheng, Y=rp1320en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11354413-
dc.identifier.scopuseid_2-s2.0-0034876027en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034876027&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume94en_HK
dc.identifier.issue5en_HK
dc.identifier.spage799en_HK
dc.identifier.epage805en_HK
dc.identifier.isiWOS:000168524600016-

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