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Article: Construction of high expression plasmid of human augmenter of liver regeneration(hALR), expression and purification of hALR

TitleConstruction of high expression plasmid of human augmenter of liver regeneration(hALR), expression and purification of hALR
Authors
KeywordsAugmenter Of Liver Regeneration
Fad-Dependent Sulfhydryl Oxidase
Prokayotic Expression Plasmid
Issue Date2006
PublisherJilin Daxue. The Journal's web site is located at http://www.cjcu.jlu.edu.cn/
Citation
Chemical Research in Chinese Universities, 2006, v. 22 n. 2, p. 201-204 How to Cite?
AbstractExperimental evidence has been presented to suggest that the human augmenter of liver regeneration (hALR) serves as a hepatotrophic growth factor during liver regeneration and as a generalized growth factor during pancreas transplant/regeneration. A prokaryotic expression plasmid, pRSET/6his-c-myc-hALR was constructed, by cloning synthesized hALR cDNA into pRSET/6his-c-myc that was improved on the basis of pRSET B by the group. As a result, the protein was highly expressed in E. coli BL21. The recombinant hALR was over 60% of the total protein in E. coli. Its validity was confirmed by means of Western Blotting. The protein was purified by Ni-NTA affinity chromatography and this FAD-dependent sulfhydryl oxidase activity was measured.
Persistent Identifierhttp://hdl.handle.net/10722/91241
ISSN
2015 Impact Factor: 1.086
2015 SCImago Journal Rankings: 0.255
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSun, T-Xen_HK
dc.contributor.authorWu, Y-Gen_HK
dc.contributor.authorYu, X-Hen_HK
dc.contributor.authorJiang, C-Len_HK
dc.contributor.authorJin, Y-Hen_HK
dc.contributor.authorCheng, Yen_HK
dc.contributor.authorKong, Wen_HK
dc.date.accessioned2010-09-17T10:15:29Z-
dc.date.available2010-09-17T10:15:29Z-
dc.date.issued2006en_HK
dc.identifier.citationChemical Research in Chinese Universities, 2006, v. 22 n. 2, p. 201-204en_HK
dc.identifier.issn1005-9040en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91241-
dc.description.abstractExperimental evidence has been presented to suggest that the human augmenter of liver regeneration (hALR) serves as a hepatotrophic growth factor during liver regeneration and as a generalized growth factor during pancreas transplant/regeneration. A prokaryotic expression plasmid, pRSET/6his-c-myc-hALR was constructed, by cloning synthesized hALR cDNA into pRSET/6his-c-myc that was improved on the basis of pRSET B by the group. As a result, the protein was highly expressed in E. coli BL21. The recombinant hALR was over 60% of the total protein in E. coli. Its validity was confirmed by means of Western Blotting. The protein was purified by Ni-NTA affinity chromatography and this FAD-dependent sulfhydryl oxidase activity was measured.en_HK
dc.languageengen_HK
dc.publisherJilin Daxue. The Journal's web site is located at http://www.cjcu.jlu.edu.cn/en_HK
dc.relation.ispartofChemical Research in Chinese Universitiesen_HK
dc.subjectAugmenter Of Liver Regenerationen_HK
dc.subjectFad-Dependent Sulfhydryl Oxidaseen_HK
dc.subjectProkayotic Expression Plasmiden_HK
dc.titleConstruction of high expression plasmid of human augmenter of liver regeneration(hALR), expression and purification of hALRen_HK
dc.typeArticleen_HK
dc.identifier.emailCheng, Y:yuecheng@hku.hken_HK
dc.identifier.authorityCheng, Y=rp1320en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S1005-9040(06)60076-7en_HK
dc.identifier.scopuseid_2-s2.0-33646381349en_HK
dc.identifier.volume22en_HK
dc.identifier.issue2en_HK
dc.identifier.spage201en_HK
dc.identifier.epage204en_HK
dc.identifier.isiWOS:000236560900018-

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