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Article: Determination of loperamide hydrochloride in human plasma using LC-MS/MS

TitleDetermination of loperamide hydrochloride in human plasma using LC-MS/MS
Authors
KeywordsForensic Toxicology Analysis
Human Plasma
Lc-Ms/Ms
Loperamide Hydrochloride
Issue Date2009
Citation
Chinese Journal of Forensic Medicine, 2009, v. 24 n. 1, p. 42-44 How to Cite?
AbstractObjective A LC-MS/MS method for analysis of loperamide hydrochloride in human plasma was developed. Methods Loperamide hydrochloride and berberine hydrochloride (internal standard) were separated from human plasma samples by liquid-liquid extraction with methanol. Chromatographic separation was performed on a ZORBAX SB-C18(2. 1mm × 150mm × 5μm) column at 35°C. Acetonitrile/0. 1% formic acid (60: 40 by volume) was used as mobile phase at a flow rate of 0. 4mL/min, and the injection volume was 10μL. Electrospray ionization(ESI) source was applied and operated in positive ion mode. Quantification was performed using multiple reaction monitoring (MRM) of the transitions m/z 477→266 and m/z 366→ 292 for loperamide hydrochloride and the internal standard, respectively. Results The limit of detection of loperamide hydrochloride was 0. 2ng/mL (S/N = 3), and the calibration curve in the concentration range of 0.5 - 500ng/mL showed a good linear (r = 0. 9982). The average recoveries of low, medium and high concentrations(1 ng/mL, 20ng/mL, 400ng/mL) were 84. 6%, 88.5% and 90. 2%, respectively. The precisions (RSD) of winth-day and between-day were less than 6% and 7%, respectively. Conclusion This LC-MS/MS method can be used for qualitative and quantitative analysis of loperamide hydrochloride.
Persistent Identifierhttp://hdl.handle.net/10722/91126
ISSN
2015 SCImago Journal Rankings: 0.103
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Xen_HK
dc.contributor.authorLin, Den_HK
dc.contributor.authorLi, Yen_HK
dc.contributor.authorYe, Fen_HK
dc.contributor.authorLin, Ben_HK
dc.date.accessioned2010-09-17T10:13:25Z-
dc.date.available2010-09-17T10:13:25Z-
dc.date.issued2009en_HK
dc.identifier.citationChinese Journal of Forensic Medicine, 2009, v. 24 n. 1, p. 42-44en_HK
dc.identifier.issn1001-5728en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91126-
dc.description.abstractObjective A LC-MS/MS method for analysis of loperamide hydrochloride in human plasma was developed. Methods Loperamide hydrochloride and berberine hydrochloride (internal standard) were separated from human plasma samples by liquid-liquid extraction with methanol. Chromatographic separation was performed on a ZORBAX SB-C18(2. 1mm × 150mm × 5μm) column at 35°C. Acetonitrile/0. 1% formic acid (60: 40 by volume) was used as mobile phase at a flow rate of 0. 4mL/min, and the injection volume was 10μL. Electrospray ionization(ESI) source was applied and operated in positive ion mode. Quantification was performed using multiple reaction monitoring (MRM) of the transitions m/z 477→266 and m/z 366→ 292 for loperamide hydrochloride and the internal standard, respectively. Results The limit of detection of loperamide hydrochloride was 0. 2ng/mL (S/N = 3), and the calibration curve in the concentration range of 0.5 - 500ng/mL showed a good linear (r = 0. 9982). The average recoveries of low, medium and high concentrations(1 ng/mL, 20ng/mL, 400ng/mL) were 84. 6%, 88.5% and 90. 2%, respectively. The precisions (RSD) of winth-day and between-day were less than 6% and 7%, respectively. Conclusion This LC-MS/MS method can be used for qualitative and quantitative analysis of loperamide hydrochloride.en_HK
dc.languageengen_HK
dc.relation.ispartofChinese Journal of Forensic Medicineen_HK
dc.subjectForensic Toxicology Analysisen_HK
dc.subjectHuman Plasmaen_HK
dc.subjectLc-Ms/Msen_HK
dc.subjectLoperamide Hydrochlorideen_HK
dc.titleDetermination of loperamide hydrochloride in human plasma using LC-MS/MSen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-61349154877en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-61349154877&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue1en_HK
dc.identifier.spage42en_HK
dc.identifier.epage44en_HK

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