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Article: Characterization and comparative analyses of zebrafish intelectins: Highly conserved sequences, diversified structures and functions

TitleCharacterization and comparative analyses of zebrafish intelectins: Highly conserved sequences, diversified structures and functions
Authors
KeywordsSpecies Index: Aeromonas Salmonicida
Danio Rerio
Deuterostomia
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/fsi
Citation
Fish and Shellfish Immunology, 2009, v. 26 n. 3, p. 396-405 How to Cite?
AbstractIntelectin family, also called the X-lectin family, is a newly discovered gene family involved in development and innate immunity. However, no research was carried out for this gene family in the model organism zebrafish. Here we present the first characterization of seven zebrafish intelectins (zINTLs) and the first systematic comparative analysis of intelectins from various species in order to provide some clues to the function and evolution of this gene family. We examined the expression patterns of zINTLs in various development stages, normal adults, and Aeromonas salmonicida infected adults. Results showed that zINTL1-3 were highly expressed in one or several adult tissues. zINTL4-7, however, were expressed at quite low levels both in adults and various development stages, and some of them showed relaxation of functional constrains as revealed by Ka/Ks calculation. Of the seven zINTLs, zINTL3 was expressed predominantly in the liver and highly up-regulated upon infection, suggesting its important roles in immunity. Based on the characterization of zebrafish intelectins, we then conducted a systematic survey of intelectin members in various species and made comparative analyses. We found out that intelectin family may be a deuterostome specific gene family; and their expression patterns, quaternary structures and glycosylations vary considerably among various species, though their sequences are highly conserved. Moreover, these varied features have evolved multiple times independently in different species, resulting in species-specific protein structures and expression patterns. © 2008 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/91104
ISSN
2015 Impact Factor: 3.025
2015 SCImago Journal Rankings: 1.311
ISI Accession Number ID
Funding AgencyGrant Number
National Basic Research Program (973)2007CB815800
State High-Tech Development Project (863)2006AA09Z433
Ministry of Science and Technology of China2007DFA30840
Ministry of Education of China0107
Commission of Science and Technology of Guangdong Province and Guangzhou City
Sun Yat-sen University Science Foundation
Funding Information:

This work was supported by Project 2007CB815800 of the National Basic Research Program (973) and Project 2006AA09Z433 of the State High-Tech Development Project (863), Project 2007DFA30840 of International S&T Cooperation Program of China from the Ministry of Science and Technology of China, Key Project (0107) from the Ministry of Education of China, and Key Projects of Commission of Science and Technology of Guangdong Province and Guangzhou City, and Project of Sun Yat-sen University Science Foundation.

References

 

DC FieldValueLanguage
dc.contributor.authorLin, Ben_HK
dc.contributor.authorCao, Zen_HK
dc.contributor.authorSu, Pen_HK
dc.contributor.authorZhang, Hen_HK
dc.contributor.authorLi, Men_HK
dc.contributor.authorLin, Yen_HK
dc.contributor.authorZhao, Den_HK
dc.contributor.authorShen, Yen_HK
dc.contributor.authorJing, Cen_HK
dc.contributor.authorChen, Sen_HK
dc.contributor.authorXu, Aen_HK
dc.date.accessioned2010-09-17T10:13:05Z-
dc.date.available2010-09-17T10:13:05Z-
dc.date.issued2009en_HK
dc.identifier.citationFish and Shellfish Immunology, 2009, v. 26 n. 3, p. 396-405en_HK
dc.identifier.issn1050-4648en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91104-
dc.description.abstractIntelectin family, also called the X-lectin family, is a newly discovered gene family involved in development and innate immunity. However, no research was carried out for this gene family in the model organism zebrafish. Here we present the first characterization of seven zebrafish intelectins (zINTLs) and the first systematic comparative analysis of intelectins from various species in order to provide some clues to the function and evolution of this gene family. We examined the expression patterns of zINTLs in various development stages, normal adults, and Aeromonas salmonicida infected adults. Results showed that zINTL1-3 were highly expressed in one or several adult tissues. zINTL4-7, however, were expressed at quite low levels both in adults and various development stages, and some of them showed relaxation of functional constrains as revealed by Ka/Ks calculation. Of the seven zINTLs, zINTL3 was expressed predominantly in the liver and highly up-regulated upon infection, suggesting its important roles in immunity. Based on the characterization of zebrafish intelectins, we then conducted a systematic survey of intelectin members in various species and made comparative analyses. We found out that intelectin family may be a deuterostome specific gene family; and their expression patterns, quaternary structures and glycosylations vary considerably among various species, though their sequences are highly conserved. Moreover, these varied features have evolved multiple times independently in different species, resulting in species-specific protein structures and expression patterns. © 2008 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/fsien_HK
dc.relation.ispartofFish and Shellfish Immunologyen_HK
dc.subjectSpecies Index: Aeromonas Salmonicidaen_HK
dc.subjectDanio Rerioen_HK
dc.subjectDeuterostomiaen_HK
dc.titleCharacterization and comparative analyses of zebrafish intelectins: Highly conserved sequences, diversified structures and functionsen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.fsi.2008.11.019en_HK
dc.identifier.pmid19100836-
dc.identifier.scopuseid_2-s2.0-62749176361en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-62749176361&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume26en_HK
dc.identifier.issue3en_HK
dc.identifier.spage396en_HK
dc.identifier.epage405en_HK
dc.identifier.eissn1095-9947-
dc.identifier.isiWOS:000265518300007-

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