File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Molecular Organization of Large Fragments in the Maize B Chromosome: Indication of a Novel Repeat

TitleMolecular Organization of Large Fragments in the Maize B Chromosome: Indication of a Novel Repeat
Authors
KeywordsSpecies Index: Acari
Embryophyta
Transposons
Zea Mays
Issue Date2004
PublisherGenetics Society of America. The Journal's web site is located at http://www.genetics.org/contents-by-date.0.shtml
Citation
Genetics, 2004, v. 166 n. 4, p. 1947-1961 How to Cite?
AbstractThe supernumerary B chromosome has no apparent effects on plant growth, and its molecular makeup is difficult to unravel, due to its high homology to the normal complement, which prevents conventional cloning. This difficulty was overcome previously by microdissecting the B chromosome under the microscope to result in 19 B clones, one of which is B specific and highly repetitive, dispersing over one-third of the B long arm and most regions of the centromeric knob. To gain insights into the molecular structure of the B chromosome, this sequence was used to screen a genomic library constructed from W22 carrying 16 B's. Five clones (>10 kb each) were isolated, and all were repetitive, showing homology with A chromosomes in Southern and FISH analyses. Two of them were further characterized and sequenced. Each is composed of several restriction fragments with variable degrees of repetitiveness. Some of these are B specific and others have variable degrees of homology with the A chromosomes. The order of each characteristic group is not contiguous; they intersperse within those of other groups. Sequence analysis reveals that their sequences (∼26 kb) have no homology with any published gene other than sequences of transposable elements (retrotransposons and MITEs) and the B as well as the A centromeres. We uncovered a 1.6-kb CL-repeat sequence, seven units of which were present in the two clones in defective forms. Those repeats mostly arrange in tandem array in the B chromosome. Moreover, we detected transposition of a retrotransposon and a MITE element involved in the genesis of these two sequences.
Persistent Identifierhttp://hdl.handle.net/10722/91101
ISSN
2015 Impact Factor: 4.644
2015 SCImago Journal Rankings: 4.270
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheng, Y-Men_HK
dc.contributor.authorLin, B-Yen_HK
dc.date.accessioned2010-09-17T10:13:03Z-
dc.date.available2010-09-17T10:13:03Z-
dc.date.issued2004en_HK
dc.identifier.citationGenetics, 2004, v. 166 n. 4, p. 1947-1961en_HK
dc.identifier.issn0016-6731en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91101-
dc.description.abstractThe supernumerary B chromosome has no apparent effects on plant growth, and its molecular makeup is difficult to unravel, due to its high homology to the normal complement, which prevents conventional cloning. This difficulty was overcome previously by microdissecting the B chromosome under the microscope to result in 19 B clones, one of which is B specific and highly repetitive, dispersing over one-third of the B long arm and most regions of the centromeric knob. To gain insights into the molecular structure of the B chromosome, this sequence was used to screen a genomic library constructed from W22 carrying 16 B's. Five clones (>10 kb each) were isolated, and all were repetitive, showing homology with A chromosomes in Southern and FISH analyses. Two of them were further characterized and sequenced. Each is composed of several restriction fragments with variable degrees of repetitiveness. Some of these are B specific and others have variable degrees of homology with the A chromosomes. The order of each characteristic group is not contiguous; they intersperse within those of other groups. Sequence analysis reveals that their sequences (∼26 kb) have no homology with any published gene other than sequences of transposable elements (retrotransposons and MITEs) and the B as well as the A centromeres. We uncovered a 1.6-kb CL-repeat sequence, seven units of which were present in the two clones in defective forms. Those repeats mostly arrange in tandem array in the B chromosome. Moreover, we detected transposition of a retrotransposon and a MITE element involved in the genesis of these two sequences.en_HK
dc.languageengen_HK
dc.publisherGenetics Society of America. The Journal's web site is located at http://www.genetics.org/contents-by-date.0.shtmlen_HK
dc.relation.ispartofGeneticsen_HK
dc.subjectSpecies Index: Acarien_HK
dc.subjectEmbryophytaen_HK
dc.subjectTransposonsen_HK
dc.subjectZea Maysen_HK
dc.titleMolecular Organization of Large Fragments in the Maize B Chromosome: Indication of a Novel Repeaten_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1534/genetics.166.4.1947en_HK
dc.identifier.pmid15126411-
dc.identifier.scopuseid_2-s2.0-2442572057en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2442572057&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume166en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1947en_HK
dc.identifier.epage1961en_HK
dc.identifier.isiWOS:000221377700030-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats