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Article: The involvement of replication in single stranded oligonucleotide-mediated gene repair

TitleThe involvement of replication in single stranded oligonucleotide-mediated gene repair
Authors
KeywordsSpecies Index: Bacteria (Microorganisms)
Escherichia Coli
Eukaryota
Mammalia
Prokaryota
Issue Date2006
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 2006, v. 34 n. 21, p. 6183-6194 How to Cite?
AbstractTargeted gene repair mediated by single-stranded oligonucleotides (SSOs) has great potential for use in functional genomic studies and gene therapy. Genetic changes have been created using this approach in a number of prokaryotic and eukaryotic systems, including mouse embryonic stem cells. However, the underlying mechanisms remain to be fully established. In one of the current models, the "annealing-integration" model, the SSO anneals to its target locus at the replication fork, serving as a primer for subsequent DNA synthesis mediated by the host replication machinery. Using a λ-Red recombination-based system in the bacterium Escherichia coli , we systematically examined several fundamental premises that form the mechanistic basis of this model. Our results provide direct evidence strongly suggesting that SSO-mediated gene repair is mechanistically linked to the process of DNA replication, and most likely involves a replication intermediate. These findings will help guide future experiments involving SSO-mediated gene repair in mammalian and prokaryotic cells, and suggest several mechanisms by which the efficiencies may be reliably and substantially increased. © 2006 Oxford University Press.
Persistent Identifierhttp://hdl.handle.net/10722/91065
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuen, MSYen_HK
dc.contributor.authorLi, XTen_HK
dc.contributor.authorLu, LYen_HK
dc.contributor.authorWatt, RMen_HK
dc.contributor.authorLiu, DPen_HK
dc.contributor.authorHuang, JDen_HK
dc.date.accessioned2010-09-17T10:12:31Z-
dc.date.available2010-09-17T10:12:31Z-
dc.date.issued2006en_HK
dc.identifier.citationNucleic Acids Research, 2006, v. 34 n. 21, p. 6183-6194en_HK
dc.identifier.issn0305-1048en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91065-
dc.description.abstractTargeted gene repair mediated by single-stranded oligonucleotides (SSOs) has great potential for use in functional genomic studies and gene therapy. Genetic changes have been created using this approach in a number of prokaryotic and eukaryotic systems, including mouse embryonic stem cells. However, the underlying mechanisms remain to be fully established. In one of the current models, the "annealing-integration" model, the SSO anneals to its target locus at the replication fork, serving as a primer for subsequent DNA synthesis mediated by the host replication machinery. Using a λ-Red recombination-based system in the bacterium Escherichia coli , we systematically examined several fundamental premises that form the mechanistic basis of this model. Our results provide direct evidence strongly suggesting that SSO-mediated gene repair is mechanistically linked to the process of DNA replication, and most likely involves a replication intermediate. These findings will help guide future experiments involving SSO-mediated gene repair in mammalian and prokaryotic cells, and suggest several mechanisms by which the efficiencies may be reliably and substantially increased. © 2006 Oxford University Press.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_HK
dc.relation.ispartofNucleic Acids Researchen_HK
dc.subjectSpecies Index: Bacteria (Microorganisms)en_HK
dc.subjectEscherichia Colien_HK
dc.subjectEukaryotaen_HK
dc.subjectMammaliaen_HK
dc.subjectProkaryotaen_HK
dc.titleThe involvement of replication in single stranded oligonucleotide-mediated gene repairen_HK
dc.typeArticleen_HK
dc.identifier.emailHuen, MSY:huen.michael@hku.hken_HK
dc.identifier.emailWatt, RM:rmwatt@hku.hken_HK
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_HK
dc.identifier.authorityHuen, MSY=rp01336en_HK
dc.identifier.authorityWatt, RM=rp00043en_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1093/nar/gkl852en_HK
dc.identifier.pmid17088285-
dc.identifier.pmcidPMC1693898-
dc.identifier.scopuseid_2-s2.0-33845614522en_HK
dc.identifier.hkuros128574-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33845614522&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume34en_HK
dc.identifier.issue21en_HK
dc.identifier.spage6183en_HK
dc.identifier.epage6194en_HK
dc.identifier.eissn1362-4962-
dc.identifier.isiWOS:000242716800017-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHuen, MSY=23004751500en_HK
dc.identifier.scopusauthoridLi, XT=8108660200en_HK
dc.identifier.scopusauthoridLu, LY=8686996700en_HK
dc.identifier.scopusauthoridWatt, RM=7102907536en_HK
dc.identifier.scopusauthoridLiu, DP=21934191400en_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK
dc.identifier.citeulike987790-

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