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Article: Expression and distribution of ionotropic glutamate receptor subunits on parasol ganglion cells in the primate retina

TitleExpression and distribution of ionotropic glutamate receptor subunits on parasol ganglion cells in the primate retina
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2002
PublisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=VNS
Citation
Visual Neuroscience, 2002, v. 19 n. 4, p. 453-465 How to Cite?
AbstractThe response properties of postreceptoral sensory neurones are determined by the properties of their input neurones, by intrinsic membrane properties, and by the properties of neurotransmitter receptors on the soma and dendritic tree. We previously showed that inhibitory neurotransmitter (GABAA and glycine) receptors on a well-characterised sensory neurone, the parasol ganglion cell in the primate retina, are segregated towards the distal part of the dendritic tree. Here we studied the distribution of excitatory ionotropic glutamate receptor subunits on the dendrites of parasol cells in the retina of a New World monkey, the marmoset, Callithrix jacchus. Individual ganglion cells were intracellularly injected in an in vitro retinal wholemount preparation. Ionotropic glutamate receptor subunits. including AMPA (GluR1-4), kainate (GluR6/7), NMDA (NR1C2′) subunits, and the orphan receptors δ1 and δ2 were visualized with immunocytochemical methods. Immunoreactive puncta that colocalized with the dendrites of ganglion cells were analyzed using standard and/or confocal light microscopy. Colocalized puncta were present on parasol dendrites for all subunits studied, but their density was much lower (approximately 1/5) than previously reported for inhibitory (GABA and glycine) receptors. Segregation of the glutamate receptor clusters (GluR1, GluR6/7 subunits) to the peripheral dendrites was less marked than that shown for GABA and glycine receptor clusters. No sign of segregation of colocalized puncta to the peripheral part of the dendritic field was seen with antibodies to the GluR2, GluR2/3, GluR4, δ1/2, or NR1C2′ subunits. The results suggest that although there is diverse expression of glutamate receptor subtypes, the glutamatergic synapses form only a small proportion of the total synaptic input to primate ganglion cells. They further suggest that the processes which control distribution of excitatory and inhibitory synapses on the dendritic field of ganglion cells are, at least to some extent, independent.
Persistent Identifierhttp://hdl.handle.net/10722/91055
ISSN
2015 Impact Factor: 1.871
2015 SCImago Journal Rankings: 1.231
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLin, Ben_HK
dc.contributor.authorMartin, PRen_HK
dc.contributor.authorGrünert, Uen_HK
dc.date.accessioned2010-09-17T10:12:22Z-
dc.date.available2010-09-17T10:12:22Z-
dc.date.issued2002en_HK
dc.identifier.citationVisual Neuroscience, 2002, v. 19 n. 4, p. 453-465en_HK
dc.identifier.issn0952-5238en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91055-
dc.description.abstractThe response properties of postreceptoral sensory neurones are determined by the properties of their input neurones, by intrinsic membrane properties, and by the properties of neurotransmitter receptors on the soma and dendritic tree. We previously showed that inhibitory neurotransmitter (GABAA and glycine) receptors on a well-characterised sensory neurone, the parasol ganglion cell in the primate retina, are segregated towards the distal part of the dendritic tree. Here we studied the distribution of excitatory ionotropic glutamate receptor subunits on the dendrites of parasol cells in the retina of a New World monkey, the marmoset, Callithrix jacchus. Individual ganglion cells were intracellularly injected in an in vitro retinal wholemount preparation. Ionotropic glutamate receptor subunits. including AMPA (GluR1-4), kainate (GluR6/7), NMDA (NR1C2′) subunits, and the orphan receptors δ1 and δ2 were visualized with immunocytochemical methods. Immunoreactive puncta that colocalized with the dendrites of ganglion cells were analyzed using standard and/or confocal light microscopy. Colocalized puncta were present on parasol dendrites for all subunits studied, but their density was much lower (approximately 1/5) than previously reported for inhibitory (GABA and glycine) receptors. Segregation of the glutamate receptor clusters (GluR1, GluR6/7 subunits) to the peripheral dendrites was less marked than that shown for GABA and glycine receptor clusters. No sign of segregation of colocalized puncta to the peripheral part of the dendritic field was seen with antibodies to the GluR2, GluR2/3, GluR4, δ1/2, or NR1C2′ subunits. The results suggest that although there is diverse expression of glutamate receptor subtypes, the glutamatergic synapses form only a small proportion of the total synaptic input to primate ganglion cells. They further suggest that the processes which control distribution of excitatory and inhibitory synapses on the dendritic field of ganglion cells are, at least to some extent, independent.en_HK
dc.languageengen_HK
dc.publisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=VNSen_HK
dc.relation.ispartofVisual Neuroscienceen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCallithrixen_HK
dc.subject.meshDendrites - metabolismen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMicroscopy, Confocalen_HK
dc.subject.meshReceptors, Glutamate - metabolismen_HK
dc.subject.meshRetinal Ganglion Cells - metabolismen_HK
dc.subject.meshSynapses - metabolismen_HK
dc.subject.meshTissue Distributionen_HK
dc.titleExpression and distribution of ionotropic glutamate receptor subunits on parasol ganglion cells in the primate retinaen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.identifier.authorityLin, B=rp01356en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1017/S0952523802194077en_HK
dc.identifier.pmid12511078-
dc.identifier.scopuseid_2-s2.0-0036663741en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036663741&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue4en_HK
dc.identifier.spage453en_HK
dc.identifier.epage465en_HK
dc.identifier.isiWOS:000179829600007-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLin, B=36165916900en_HK
dc.identifier.scopusauthoridMartin, PR=7406040488en_HK
dc.identifier.scopusauthoridGrünert, U=7004613233en_HK

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