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- Publisher Website: 10.1016/S0006-8993(02)03429-7
- Scopus: eid_2-s2.0-0037111397
- PMID: 12419533
- WOS: WOS:000179424800019
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Article: Interactions between recording technique and AMPA receptor modulators
Title | Interactions between recording technique and AMPA receptor modulators |
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Authors | |
Keywords | Chemicals And Cas Registry Numbers |
Issue Date | 2002 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres |
Citation | Brain Research, 2002, v. 955 n. 1-2, p. 164-173 How to Cite? |
Abstract | Whole cell recording (EPSCs) and extracellular recording (field EPSPs) were compared in hippocampal field CA1 with regard to the effects of experimental treatments that increase AMPA receptor gated currents. Cyclothiazide, which maintains AMPA receptors in the sensitized state, caused a rapid and pronounced increase in EPSCs but only minor changes in field EPSPs. This difference was evident in recordings carried out at 22 and 32°C and with different solutions in the clamp pipette. The larger effect of cyclothiazide on EPSCs was unaffected by blockade of GABA and NMDA receptors. Two-dimensional current source density analyses derived from 64 recording sites were used to provide extracellular estimates of AMPA receptor mediated synaptic currents. With this method, cyclothiazide again had much smaller effects than were obtained with whole cell clamp. Differences between whole cell and extracellular recordings were present, although not as pronounced, for the ampakines, a class of drugs that slow both deactivation and desensitization of AMPA receptors. Additionally, increases in synaptic responses produced by frequency facilitation, a manipulation that enhances the number of bound receptors, were not qualitatively different between recording techniques. These results support the conclusion that the whole cell clamp technique may alter AMPA receptors in such a way as to increase the relative importance of desensitization. © 2002 Elsevier Science B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/91013 |
ISSN | 2023 Impact Factor: 2.7 2023 SCImago Journal Rankings: 0.832 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Lin, B | en_HK |
dc.contributor.author | Colgin, LL | en_HK |
dc.contributor.author | Brücher, FA | en_HK |
dc.contributor.author | Arai, AC | en_HK |
dc.contributor.author | Lynch, G | en_HK |
dc.date.accessioned | 2010-09-17T10:11:44Z | - |
dc.date.available | 2010-09-17T10:11:44Z | - |
dc.date.issued | 2002 | en_HK |
dc.identifier.citation | Brain Research, 2002, v. 955 n. 1-2, p. 164-173 | en_HK |
dc.identifier.issn | 0006-8993 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/91013 | - |
dc.description.abstract | Whole cell recording (EPSCs) and extracellular recording (field EPSPs) were compared in hippocampal field CA1 with regard to the effects of experimental treatments that increase AMPA receptor gated currents. Cyclothiazide, which maintains AMPA receptors in the sensitized state, caused a rapid and pronounced increase in EPSCs but only minor changes in field EPSPs. This difference was evident in recordings carried out at 22 and 32°C and with different solutions in the clamp pipette. The larger effect of cyclothiazide on EPSCs was unaffected by blockade of GABA and NMDA receptors. Two-dimensional current source density analyses derived from 64 recording sites were used to provide extracellular estimates of AMPA receptor mediated synaptic currents. With this method, cyclothiazide again had much smaller effects than were obtained with whole cell clamp. Differences between whole cell and extracellular recordings were present, although not as pronounced, for the ampakines, a class of drugs that slow both deactivation and desensitization of AMPA receptors. Additionally, increases in synaptic responses produced by frequency facilitation, a manipulation that enhances the number of bound receptors, were not qualitatively different between recording techniques. These results support the conclusion that the whole cell clamp technique may alter AMPA receptors in such a way as to increase the relative importance of desensitization. © 2002 Elsevier Science B.V. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres | en_HK |
dc.relation.ispartof | Brain Research | en_HK |
dc.subject | Chemicals And Cas Registry Numbers | en_HK |
dc.title | Interactions between recording technique and AMPA receptor modulators | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Lin, B:blin@hku.hk | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/S0006-8993(02)03429-7 | en_HK |
dc.identifier.pmid | 12419533 | - |
dc.identifier.scopus | eid_2-s2.0-0037111397 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0037111397&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 955 | en_HK |
dc.identifier.issue | 1-2 | en_HK |
dc.identifier.spage | 164 | en_HK |
dc.identifier.epage | 173 | en_HK |
dc.identifier.isi | WOS:000179424800019 | - |
dc.identifier.issnl | 0006-8993 | - |